TwistAmp exo, real-time fluorescent DNA detection
The exo kit is recommended for users who want to combine the TwistDx RPA amplification technology with the use of TwistDx's proprietary fluorescent TwistAmp® exo Probe in a homogenous format.
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For magnetic mixing using the T16 device, bearing balls may be added to reactions at any time before the reaction is initiated with Mg.
In addition to the basic components it contains a powerful nuclease (Exonuclease III) which will process TwistAmp® exo Probe during the amplification reaction itself and generate a real-time read-out. The presence of Exonuclease III will reduce the final overall yield of amplified material at endpoint and so is not suitable for analysis on gels, however, it is the preferred system for generating strong fluorescence signal kinetics in the RPA system.
There are 96 reactions in each kit.
NB Due to current production methods, RPA reactions are not suitable for amplification of standard laboratory strains of E. coli. If you are interested in amplifying E. coli DNA, please contact email@example.com
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