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TwistAmp® nfo - Lateral flow DNA detection

The nfo kit is designed for users who want to detect the success of their DNA amplification reaction by means of end-point ‘sandwich-assays’, such as lateral-flow (LF) strips.

This kit includes extra ssDNA binding protein and dNTP to improve RPA performance. We highly recommend customers switch to this formulation as we expect improved performance for most uses.


  • End-point detection
  • Single-molecule detection
  • Lateral flow dipstick compatible
  • Multipleaxable
  • 1 x box of 96 reactions

Purchasing Information:

Price: £240     €340     $410

Product Code: TANFO02KIT

Final price will be confirmed during checkout

Old Formulation TwistAmp® nfo


  • Single-molecule detection
  • ​Lateral flow dipstick compatible
  • 1 x box of 96 reactions

Purchasing Information:

Price: £240     €340     $410

Product Code: TANFO01KIT



For magnetic mixing using the Twirla, bearing balls may be added to reactions at any time before the reaction is initiated with Mg.

Besides the basic amplification reagents it includes a nuclease (nfo) which can generate new polymerase extension substrates within suitable LF TwistAmp® Probes  – the amplified material can then be used in instrument-free detection formats. The TwistAmp® nfo kit can also be used for fluorescence monitoring using fluorescent exo TwistAmp® exo Probe  as an alternative to the TwistAmp® exo kit as nfo will process these probes (often with slightly slower kinetics) and will permit product to be analysed by gels at endpoint in addition.

There are 96 reactions in each kit.

NB Due to current production methods, RPA reactions are not suitable for amplification of standard laboratory strains of E. coli. If you are interested in amplifying E. coli DNA, please contact

Need LATERAL FLOW STRIPS? Visit this website to purchase

Download oligonucleotide order forms:

TwistAmp® nfo Probe Order Form BIOSEARCH

Biosearch Custom Oligo Order Form

Eurogentec Oligo Batch Order v7.0 TwistAmp®

TwistAmp® nfo Probe Order Form GENERIC


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