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Publications

Over 40 peer publication written about RPA

Every year more and more scientists are finding out that RPA really works. Over 100 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.
  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Reverse transcription RPA assay for the rapid detection of type 2 porcine reproductive and respir…

    Jian-chang Wang, Wan-zhe Yuan, Qing-an Han, Jin-feng Wang, Li-bing Liu

    Highlights

    • An exo RT-RPA method was developed for the detection of classical and highly pathogenic PRRSV.
    • RT-RPA showed high sensitivity and specificity in PRRSV detection.
    • The method was further validated using clinical samples.
    • RT-RPA was shown to be a simple, rapid and reliable method for PRRSV detection.

    Read more...

  • Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection

    Han Yih Lau, Haoqi Wu, Eugene J. H. Wee, Matt Trau, Yuling Wang, Jose R. Botella

    From abstract:
    Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathogen DNA sequences by recombinase polymerase amplification (RPA) followed by gold nanoparticle-based electrochemical assessment with differential pulse voltammetry (DPV). 

    Product used: TwistAmp® Basic

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  • Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection

    Jinzhao Song, Changchun Liu, Michael G. Mauk, Shelley C. Rankin, James B. Lok, Robert M. Greenberg, Haim H. Bau

    From abstract:
    To address the need for point-of-care (POC) highly multiplexed tests, we propose the 2-stage, nested-like, rapid (<40 min) isothermal amplification assay, dubbed rapid amplification (RAMP). In experiments on a benchtop and in a microfluidic format, RAMP demonstrated high level of multiplexing (≥16); high sensitivity (i.e., 1 plaque-forming unit of Zika virus) and specificity (no false positives or negatives); speed (<40 min); ease of use; and ability to cope with minimally processed samples.

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  • Development of a RPA Assay for Detection of Epidemic Human Noroviruses

    Matthew D. Moore, Lee-Ann Jaykus

    From abstract:
    The assay detected norovirus in some samples in as little as 6 min, and the entire detection process can be performed in less than 30 min. The reported RT-RPA method shows promise for sensitive point-of-care detection of epidemic human norovirus, and is the fastest human norovirus amplification method to date.

    Product used: TwistAmp® exo RT

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  • Development of a RPA Assay for Detection of Epidemic Human Noroviruses

    Matthew D. Moore, Lee-Ann Jaykus

    From abstract:
    The assay detected norovirus in some samples in as little as 6 min, and the entire detection process can be performed in less than 30 min. The reported RT-RPA method shows promise for sensitive point-of-care detection of epidemic human norovirus, and is the fastest human norovirus amplification method to date.

    Product used: TwistAmp® exo RT

    Read more...

  • Simple, Fast and Cost-Effective Alternative to Real Time PCR for Specific Detection of¬†Feline Herpes

    Jianchang Wang, Libing Liu, Jinfeng Wang, Xiaoxia Sun, Wanzhe Yuan

    From abstract:
    In this study, an exo-RPA assay for FHV-1 detection was developed and validated. Primers targeting specifically the thymidine kinase (TK) gene of FHV-1 were designed. The RPA reaction was performed successfully at 39°C and the results were obtained within 20 min. 

    Product used: TwistAmp® exo

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  • Isothermal, label-free, rapid one-step RNA amplification/detection assay for diagnosis of respirator

    Bonhan Koo, Choong Eun Jin, Tae Yoon Lee, Jeong Hoon Lee et al

    Highlights

    •Employed isothermal amplification with bio-photonic sensor for detection of viral RNAs.
    •Rapid RNA amplification and detection system simultaneously within 20 min.
    •Validated the utility of the system in human respiratory viral samples.
    •Versatile technology can be readily applied to other RNA-based studies and diseases.

    Product used: TwistAmp® Basic

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  • Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus

    Mohamed A. Shalaby, Ayman El-Deeb, Mohamed El-Tholoth Ahmed Abd El Wahed et al

    Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed.

    Product used: TwistAmp® exo

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  • Development of a RPA Assay for Rapid Detection of the Mycobacterium avium¬†subsp.¬†paratuberculosis

    Sören Hansen, Jenny Schäfer, Kim Fechner, Claus-Peter Czerny, Ahmed Abd El Wahed

    From abstract:
    In this study, we have developed a rapid assay for the detection of MAP based on the recombinase polymerase amplification (RPA) assay targeting a MAP specific region, the IS900 gene. The detection limit was 16 DNA molecules in 15 minutes as determined by the probit analysis on eight runs of the plasmid standard. All results were compared with reads of a highly sensitive real-time PCR assay.

    Product used: TwistAmp® exo

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  • Detection of ESKAPE bacterial pathogens at the point-of-care using isothermal DNA- 2 based assays

    Lars D. Renner, Jindong Zan, Linda I. Hu, Douglas B. Weibel et al

    This manuscript addresses this important diagnostic technology gap by describing a low cost, portable, rapid, and easy-to-use microfluidic cartridge-based system for detecting the ESKAPE bacterial pathogens that are most commonly associated with antibiotic resistance. The isothermal RPA assays detect the targeted ESKAPE pathogens with high sensitivity (e.g., a limit of detection of ~10 nucleic acid molecules) that is comparable to current PCR-based assays

    Product used: TwistAmp® exo

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  • Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus

    Kamfai Chan, Scott C. Weaver, Pui-Yan Wong, Season Wong et al

    From abstract:
    The preliminary results of a portable and low-cost molecular diagnostics system for ZIKV infection are reported here. In less than 15 minutes, this low-cost platform can automatically perform high quality RNA extraction from up to 12 ZIKV-spiked urine samples simultaneously.

    Product used: TwistAmp® exo RT

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