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Publications

Over 40 peer publication written about RPA

Every year more and more scientists are finding out that RPA really works. Over 100 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.
  • A rapid assay for detection of Rose rosette virus using RT-RPA using multiple gene targets

    Binoy Babu, Brian K. Washburn, Steven H. Miller, Mathews L. Paret et al

    Highlights

    •Designed multiple RT-RPA primer sets for Rose rosette virus (RRV).
    •RT-RPA assay for detection of RRV was developed.
    •The RPA primer sets were highly specific to RRV.
    •Primer sets were highly sensitive, detecting up to 1 fg of virus.
    •Developed assays are rapid, sensitive and reliable.
    •Developed assays successfully detected RRV from leaves, stems and flower petals.

    Product used: TwistAmp® Basic RT

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  • Assay for Listeria monocytogenes cells in whole blood using isotachophoresis and RPA

    Charbel Eid, Juan G. Santiago

    From abstract:

    We present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA). Lysis, mixing, dispensing, and on-chip ITP purification are completed in a total of less than 50 min.

    Product used: TwistAmp® exo + ListeriaM

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  • Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

    Miriam Jauset-Rubio, Markéta Svobodová, Teresa Mairal, Ciara K. O´Sullivan et al

    From abstract:

    Here we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a duplex flanked by two single stranded DNA tails. 

    Product used: TwistAmp® Basic

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  • High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine

    Kevin M. Koo, Eugene J.H. Wee, Matt Trau

    Highlights

    •Non-invasive detection of multiple gene fusion biomarkers in patient urine samples.
    •60 min assay which is at least five times faster than traditional multiplexed ligase-based assays.
    •Requires only 30 ng of starting total RNA sample and has 2 amol detection sensitivity.
    •Accuracy validated with quantitative real-time PCR.
    •Good reproducibility at CV=8.9%.

    Product used: TwistAmp® Basic

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  • RPA Compared to Real-Time PCR Test for the Detection of Fasciola hepatica in Human Stool

    Miguel M. Cabada, Jose L. Malaga, Alejandro Castellanos-Gonzalez, A. Clinton White Jr. et al

    From abstract:
    We report on the characterization of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites. In addition, RPA and PCR were able to detect 47% and 26% of infections not detected by microscopy, respectively.

    Product used: TwistAmp® Basic and nfo

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  • Rapid molecular detection of Zika virus in urine using recombinase polymerase amplification assay

    Ahmed Abd El Wahed, Sabri S. Sanabani, Oumar Faye, Rodrigo Pessoa, Joao Veras Patriota, Ricardo Rodrigues-Giorgi, Pranav Patel, Susanne Boehlken-Fascher, Olfert Landt, Matthias Niedrig, Paolo M. de A. Zanotto, Claus-Peter Czerny, Amadou A. Sall, Manfred Weidmann

    From abstract:

    In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92% while the specificity was 100%. 

    Product used: TwistAmp® exoRT

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  • Development and evaluation of a rapid RPA assay for detection of coxsackievirus A6

    Wang K, Wu Y, Yin D, Tang S, Hu G,He Y.

    From abstract:

    The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit.

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  • Development of qRT-RPA Method for Quantitative Detection against Pathogenic Virus in Honeybee

    Min S-H, Wang J-H, Lim S-J, Lee C-W, Yoon B-S.

    From abstract:
    The existence of kSBV specific gene could be detected using total cDNA within 2 min 46 sec using this method. Based on this method, we proposed the quantitative real-time recombinase polymerase amplification which was able to quantify the target gene and to be applied universally.

    Product used: TwistAmp® Basic

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  • Ultrasensitive and rapid detection of β-conglutin combining aptamers and isothermal RPA

    Jauset-Rubio M, Sabaté del Río J, Mairal T, Svobodová M, El-Shahawi MS, Bashammakh AS, Alyoubi AO, O’Sullivan CK.

    From abstract
    An innovative method termed aptamer-recombinase polymerase amplification (Apta-RPA) exploiting the affinity and specificity of a DNA aptamer selected against the anaphylactic β-conglutin allergen termed β-conglutin binding aptamer II (β-CBA II), facilitating ultrasensitive detection via isothermal amplification. Combining magnetic beads as the solid phase with Apta-RPA detection, the total assay time was reduced from 210 min to just 25 min, with a limit of detection of 3.5 × 10−11 M, demonstrating a rapid and ultrasensitive generic methodology that can be used with any aptamer.

    Product used: TwistAmp® Basic

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  • Development of a multiplex RT-RPA assay for rapid quantitative detection of Campylobacter coli…

    Kim JY, Lee JL.

    Highlights
    •Development of a multiplex real-time RPA assay to detect Campylobacter coli & jejuni.

    •Quantitative detection within a 20 min runtime using the multiplex Rti-RPA assay.

    •Equivalent or better detection sensitivity than other PCR/LAMP assays.

    •Fast procedure, fairly simple, and specific machinery not required.

    •Applicable in the surveillance of Campylobacter contamination in poultry products.

    Product used: TwistAmp® exo

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  • Aptamer LF Assays for Ultrasensitive Detection of β-Conglutin Combining RPA & tailed primers

    Jauset-Rubio M, Svobodová M, Mairal T, McNeil C, Keegan N, El-Shahawi MS, Bashammakh AS, Alyoubi AO, and O’Sullivan CK.

    From abstract
    In this work, different methodologies were evaluated in search of robust, simple, rapid, ultrasensitive, and user-friendly lateral flow aptamer assays. In one approach, we developed a competitive based lateral flow aptamer assay, in which β-conglutin immobilized on the test line of a nitrocellulose membrane and β-conglutin in the test sample compete for binding to AuNP labeled aptamer.

    Product used: TwistAmp® Basic

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  • Toward Precision Medicine: Cancer Molecular Subtyping Nano-Strategy for RNA Biomarkers in Tumor & Ur

    Kevin M. Koo, Eugene J. H. Wee, Paul N. Mainwaring, Yuling Wang, Matt Trau

    From abstract:

    Herein a rapid (80 min) multiplexed platform strategy for subtyping prostate cancer tumor and urine samples based on their RNA biomarker profiles is presented. This is achieved by combining rapid multiplexed isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) of target RNA biomarkers with surface-enhanced Raman spectroscopy (SERS) nanotags for “one-pot” readout…With excellent sensitivity of 200 zmol (100 copies) and specificity, we believed that this platform methodology could be a useful tool for rapid multiplexed subtyping of cancers.

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