Contact Us
TwistDx Logo

Publications

Over 40 peer publication written about RPA

Every year more and more scientists are finding out that RPA really works. Over 100 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.
  • A phaseguided passive batch microfluidic mixing chamber for isothermal amplification

    With a view to developing a rapid pathogen detection system utilizing isothermal nucleic acid amplification, the necessary micro-mixing step is innovatively implemented on a chip. Passive laminar flow mixing of two 6.5 μl batches differing in viscosity is performed within a microfluidic chamber. This is achieved with a novel chip space-saving phaseguide design which allows, for the first time, the complete integration of a passive mixing structure into a target chamber. Sequential filling of batches prior to mixing is demonstrated. Simulation predicts a reduction of diffusive mixing time from hours down to one minute. A simple and low-cost fabrication method is used which combines dry film resist technology and direct wafer bonding. Finally, an isothermal nucleic acid detection assay is successfully implemented where fluorescence results are measured directly from the chip after a one minute mixing sequence. In combination with our previous work, this opens up the way towards a fully integrated pathogen detection system in a lab-on-a-chip format.

    Read more . . .

  • A paper & plastic device to perform recombinase polymerase amplification of HIV DNA

    We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 min. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings.

    Read more . . .

  • Optimizing Illumina next-gen sequencing library preparation for at-biased genomes

    Oyola et al compared a number of different polymerases and PCR methods with RPA for generating libraries for sequencing AT-rich genomes. The TwistAmp Basic reactions were used without optimisation, simply following the instructions in the Quick Guide. Yields were relatively high compared to T7 libraries and there was a reduced bias towards GC rich templates compared to standard Illumina libraries. Unfortunately RPA libraries showed relatively high levels of chimeric and duplicate reads.

    Read more . . .

  • Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus

    Detection of nucleic acids of Rift Valley fever virus (RVFV) by one-step-RT-RPA. RVFV RNA was detected with a sensitivity of 19 molecules detected as determined by probit analysis of eight runs using a RVFV S-segment based quantitative RNA standard and detected 20 different RVFV strains. The assays showed no cross detection of the human genome and several agents of a typical biothreat panel. The presented combination of one-step-RT-RPA and portable fluorescence reading device could be a useful tool for field or point of care diagnostics.

    Read more . . .

  • Recombinase Polymerase amplification assay for rapid detection of Francisella tularensis

    We report the development of a novel qualitative real time isothermal recombinase polymerase amplification (RPA) on a small ESEQuant tubescanner device. The analytical sensitivity and specificity was tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real time PCR but reduced assay time to 10 minutes. The rapid RPA method has great application potential for field use or point of care diagnostics.

    Read more . . .

  • Digital isothermal quantification of nucleic acids . . .

    In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel.

    Read more . . .

  • Microfluidic lab-on-a-foil for nucleic acid analysis . . .

    For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA).

    Read more . . .


  • Page 12 of 12 pages ‹ First  < 10 11 12