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Publications

Over 40 peer publication written about RPA

Every year more and more scientists are finding out that RPA really works. Over 100 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.
  • A Field-Deployable Reverse Transcription RPA Assay for Rapid Detection of Chikungunya Virus

    Pranav Patel , Ahmed Abd El Wahed , Oumar Faye, Pauline Prüger, Marco Kaiser, Sasikanya Thaloengsok, Sukathida Ubol, Anavaj Sakuntabhai, Isabelle Leparc-Goffart, Frank T. Hufert, Amadou A. Sall, Manfred Weidmann, Matthias Niedrig

    From abstract
    In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes.

    Product used: TwistAmp® exo RT

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  • Development and performance evaluation of RPA assay for the rapid detection of group B streptococcus

    Christina Clarke, Louise O’Connor,  Heather Carré-Skinner, Olaf Piepenburg and Terry J. Smith

    From abstract
    In this study, a Recombinase Polymerase Amplification (RPA) assay was developed and performance evaluated for the detection of group B Streptococcus in vaginal swabs. The assay uses the cAMP factor (cfb) gene of GBS as the target gene. The analytical performance of the assay was evaluated by testing a panel of GBS reference strains and clinical isolates, and non-GBS organisms. The limit of detection was determined and the clinical performance was evaluated by testing 124 vaginal swabs from women with both GBS positive and negative status.

    Product used: TwistAmp® Exo (custom)

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  • Rapid detection of Mycobacterium tuberculosis using recombinase polymerase amplification

    CHEN Bin, YANG Yin-mei, ZHONG Zhi-min, XU Bang-lao

    From abstract

    The detection time of RPA method for Mycobacterium tuberculosis was only 15 min. The analytical sensitivity of this method was 0.043 ng / μl genomic DNA of Mycobacterium tuberculosis strain.

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  • RPA combined with a lateral flow dipstick for rapid and visual detection of Schistosoma japonicum

    Kui Sun, Weiwei Xing, Xinling Yu, Wenliang Fu, Yuanyuan Wang, Minji Zou, Zhihong Luo and Donggang Xu

    From abstract
    With the continuous decline in prevalence and intensity of Schistosoma japonicum infection in China, more accurate and sensitive methods suitable for field detection become much needed for schistosomiasis control. The LFD-RPA assay showed 92.68 % sensitivity, 100 % specificity and excellent diagnostic agreement with the gold standard Kato-Katz test (k = 0.947, Z = 6.36, P < 0.001).

    Product used: TwistAmp® exo

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  • Rapid detection of porcine reproductive and respiratory syndrome virus by a fluorescent probe RPA

    Yang Yang, Xiaodong Qin, Yingjun Sun, Ting Chen, Zhidong Zhang

    From abstract
    A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction.

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  • Rapid Detection of Cyprinid Herpesvirus 3 in Latently Infected Koi by RPA

    Meagan A. Prescott, Aimee N. Reed, Ling Jin & Manoj K. Pastey

    From abstract
    Since the emergence of cyprinid herpesvirus 3 (CyHV-3), outbreaks have been devastating to Common Carp Cyprinus carpio and koi (a variant of CommonCarp), leading to high economic losses.. Here we describe the detection of CyHV-3 by recombinase polymerase amplification (RPA). The RPA assay can detect as low as 10 copies of the CyHV-3 genome by an isothermal reaction and yields results in approximately 20 min.

    Product used: TwistAmp® Basic

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  • 역전사 실시간 Recombinase Polymerase Amplification (RT/RT RPA)에 의한 꿀벌 Black Queen Cell Virus의 신속 검출

    (Rapid Detection of Black Queen Cell Virus from Honeybee using Reverse Transcription Real-Time Recombinase Polymerase Amplification (RT/RT RPA))

    임수진, Giang Thi Huong Luong, 민상현, 왕지희, 윤병수

    From abstract

    BQCV-specific DNA amplification could be detected from 3 min 26 sec after RPA reaction with specific DNA templates by RT-RPA, while 41 min 42 sec was required by qRTPCR with same quantities of initial templates.

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  • Simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion

    Kevin M. Koo, Eugene J. H. Wee, Paul N. Mainwaring & Matt Trau

    From abstract
    We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment.

    Product used: TwistAmp® Basic

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  • Rapid detection of Porcine circovirus-2 by recombinase polymerase amplification

    Jianchang Wang, Jinfeng Wang, Libing Liu, Ruiwen Li, Wanzhe Yuan

    From abstract
    Porcine circovirus–associated disease, caused primarily by Porcine circovirus-2 (PCV-2), has become endemic in many pig-producing countries and has resulted in significant economic losses to the swine industry worldwide. Tests for PCV-2 infection include PCR, nested PCR, competitive PCR, and real-time PCR (rtPCR). Recombinase polymerase amplification (RPA) has emerged as an isothermal gene amplification technology for the molecular detection of infectious disease agents.

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  • A Novel Isothermal Assay of Borrelia burgdorferi by Recombinase Polymerase Amplification with LF

    Wei Liu, Hui-Xin Liu, Lin Zhang, Xue-Xia Hou, Kang-Lin Wan and Qin Hao

    From abstract
    A novel isothermal detection for recombinase polymerase amplification with lateral flow (LF-RPA) was established for Borrelia burgdorferi detection in this study. This assay with high sensitivity and specificity can get a visible result without any additional equipment in 30 min.

    Product used: TwistAmp® Basic + nfo

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  • Field Demonstration of a Multiplexed Point-of-Care Diagnostic Platform for Plant Pathogens

    Han Yih Lau, Yuling Wang, Eugene J. H. Wee, Jose R. Botella, and Matt Trau

    From abstract
    a rapid, highly specific and sensitive point-of-care method for multiplex detection of plant pathogens was developed by taking advantage of surface-enhanced Raman scattering (SERS) labeled nanotags and recombinase polymerase amplification (RPA), which is a rapid isothermal amplification method with high specificity.

    Product used: TwistAmp® Basic

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  • Development and Evaluation of a Rapid and Sensitive EBOV-RPA Test for Rapid Diagnosis of Ebola Virus

    Mingjuan Yang, Yuehua Ke, Xuesong Wang et al.

    From abstract
    A rapid nucleic acid test based on recombinase polymerase amplification (EBOV-RPA) was developed to specifically detect the 2014 outbreak strains. The EBOV-RPA assay was evaluated by testing samples from suspected EVD patients in parallel with RT-PCR.

    Product used: TwistAmp® exo

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