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DNA Amplification and Detection Made Simple (Relatively)
Twenty-three years ago, a man musing about work while driving down a California highway revolutionized molecular biology when he envisioned a technique to make large numbers of copies of a piece of DNA rapidly and accurately. Known as the polymerase chain reaction, or PCR, Kary Mullis’s technique involves separating . . .
DNA Detection Using Recombination Proteins
DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited. Our novel approach, recombinase polymerase amplification (RPA), couples isothermal recombinase-driven primer targeting of template material with strand-displacement DNA synthesis. It achieves exponential amplification with no need for pretreatment of sample DNA. Reactions are sensitive, specific, and rapid and . . .
Biochemical solutions for portable nucleic acid testing
For the analysis of biological materials, nucleic acid tests (NATs) offer key advantages over alternative approaches (e.g. protein detection). NATs are capable of exquisite sensitivity (single molecule) and can be engineered to be extremely specific. Current laboratory-based applications include the . . .