One step real-time fluorescent RNA amplification in a flexible liquid format. Recommended for users who want to combine TwistDx’s RPA technology to detect RNA targets with the use of TwistDx's proprietary fluorescent TwistAmp® exo probe. It contains the reagent components of a TwistAmp® Liquid exo kit as well as a compatible reverse transcriptase, which converts the initially present RNA template into DNA and thus into amplification substrates. The user need only supply primers, probe, dNTPs, template and RNase Inhibitor. See manual for more information. Click to order oligonucleotides.
Perfect for: Real-time fluorescent RNA detection, facilitating use of different RPA reaction volumes, or variation of component ratios
NB: Due to current production methods, RPA reactions are not suitable for amplification of standard laboratory strains of E. coli. If you are interested in amplifying E. coli DNA, please contact firstname.lastname@example.org