Say goodbye, PCR! New liquid isothermal designed to entirely replace PCR reagents.

28 June 2017

TwistDx has launched a new range of liquid format, isothermal Recombinase Polymerase Amplification (RAP) kits that are ideal for high throughput laboratory use where DNA/RNA amplification is needed within 15 minutes without a thermocycler, and for researchers who want the flexibility to experiment with their RPA assay configurations.

The TwistAmp® Liquid kits provide RPA assay reagents in a glycerol-stabilized, liquid format that will be familiar to anyone who carries out PCR and other enzyme-based reactions in the laboratory. The reagents are provided in separate tubes, and users can pipette out as much or as little of the enzyme mix as they want to make up RPA reaction volumes of their choosing, create a bulk master mix as the foundation for multiple reactions, or experiment with ratios of RPA components to optimize their assays.

New TwistAmp® Liquid kits offer an alternative format to TwistDx’s flagship ambient temperature-stable lyophilized TwistAmp® RPA kits, which are ideal for the development of field and site-based assays. The liquid format will make it easy for laboratories to use RPA routinely as a faster and more user-friendly replacement to PCR, sometimes even using the same PCR primers, and to develop customized assays without a thermocycler.

The new TwistAmp® Liquid range includes TwistAmp® Liquid Basic, TwistAmp® Liquid Basic RT, TwistAmp® Liquid exo, and TwistAmp® Liquid exo RT.

The liquid format will make it easy for laboratories to use RPA routinely as a faster and more user-friendly replacement to PCR, sometimes even using the same PCR primers, and to develop customized assays without a thermocycler.