Archives: FAQs

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Will PCR primers work in RPA?

Yes. We have recently confirmed that primers of lengths typical for use in PCR (e.g. 18-23 nucleotides) work just fine in RPA in many cases. Note though that kinetics can be a little slower, so if you wish to take full advantage of the potential of RPA for rapid amplification within 10-15 minutes we recommend using slightly longer primers (as …

Will PCR primers work in RPA? Read More

When using TwistAmp® Liquid reactions how might I improve reproducibility/amplification of my RNA target?

If you are combining your RNA template with MgOAc before adding it to your reactions, you may be stabilising RNA tertiary structures that inhibit the reverse transcriptase. Adding the RNA to your reaction mixture either before you add the MgOAc, or spatially separating it (i.e. adding RNA and MgOAc to opposite sides of your reaction vessel or lid) and adding …

When using TwistAmp® Liquid reactions how might I improve reproducibility/amplification of my RNA target? Read More

What will happen when I place an order?

Delivery should be within 3 weeks of placing an order or payment. The Buyer will receive a notification of an expected delivery date upon placement of order. Upon shipping the product to the buyer, the Buyer will receive a notification from the courier company with your tracking number. The Company uses FedEx for international shipping.

What melting temperature should RPA primers have?

As RPA reactions are performed at a constant temperature and under conditions under which the melting behaviour of DNA is drastically altered by DNA melting proteins, conventionally calculated melting points are not directly applicable to the system.

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