Yes, unless you are using the TwistAmp® exo kit or exo RT kit. The exonuclease present in the reaction mixture will digest most of the amplification product once amplification has ceased. For the analysis of amplicon, use the TwistAmp® Basic kit. It is also possible to analyse products on gels generated from the TwistAmp®nfo and TwistAmp® fpg kits. NB The TwistAmp®nfo kit can be used with TwistAmp® exo probes if you would like to run these on a gel, although the kinetics will be slower.