How do I reconstitute a lyophilised probe?

Please follow the oligonucleotide manufacturer’s instructions for the reconstitution and storage of the probes. Typically the tube containing the lyophilised oligonucleotide will be spun briefly to collect the DNA at the bottom of the tube and an appropriate volume of T0.1E buffer (10mM Tris-HCI pH 8, 0.1mM EDTA) will be added to prepare a stock solution of 100μM. Allow the solution to stand for 10 minutes at room temperature and mix (vortex) for 10 seconds. Reconstituted oligonucleotide probes are typically stored at -20°C for the long term.

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