How do I select a probe?

If using one of the TwistAmp® kits suitable for probe use, follow the guidelines described in the assay design manual for the design of detection probes. Note that there are some sequence restrictions in choosing the position of probes within a target using the preferred TwistAmp® exo Probe system. If this presents unreasonable limitations TwistDx can help you design alternative detection strategies. The alternative TwistAmp® fpg Probe system is far more amenable to flexible design, but does not always yield as strong fluorescence signal as TwistAmp® exo Probe.

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