FAQs

FAQsPost Type

The 20x Core Reaction Mix contains precipitate/does not seem to be homogenous. What should I do?

The recombinase and ancillary protein present in the 20x Core Reaction Mix are known to sometimes reversibly co-precipitate in 50% glycerol when stored at low temperatures. Warming the 20x Core Reaction Mix to room temperature and mixing will return them into solution, with no loss of function.

The 20x Core Reaction Mix contains precipitate/does not seem to be homogenous. What should I do? Read More

I’ve tested some primers with one type of TwistAmp® kit; will they work with another type of TwistAmp® kit?

Maybe. If you have developed primers using a TwistAmp® Basic kit, you will also need to include a probe if you are going to use a TwistAmp® exo or nfo kit. This and other factors mean that an optimal primer combination for a gel-based approach vs a fluorescence or lateral flow approach may not be the same. In general we

I’ve tested some primers with one type of TwistAmp® kit; will they work with another type of TwistAmp® kit? Read More

I want to run multiple experiments using TwistAmp® Liquid reactions; can I make a master mix and store it?

Oligos, 2x Reaction Buffer, water, dNTPs and 10x E-mix can be combined and stored on ice for a minimum of hours, and over-night in many cases (any effects on performance will be assay dependent and should be assessed independently by users). Once the 20x Core Reaction Mix is added short term storage on ice (assay dependent again) should also be

I want to run multiple experiments using TwistAmp® Liquid reactions; can I make a master mix and store it? Read More

I sometimes see a sharp increase in fluorescence towards the end of my TwistAmp® exo (RT) reaction; is this normal?

Yes. The polymerase and exonuclease III in TwistAmp® exo reactions are in competition. As the reaction runs out of energy, the exonuclease III starts to dominate. This leads to faster probe cleavage and a jump in fluorescence signal.

I sometimes see a sharp increase in fluorescence towards the end of my TwistAmp® exo (RT) reaction; is this normal? Read More

I keep getting false positive results, what is the likely cause of this?

If positive results are seen in no template controls it is commonly caused by contamination, particularly, if the positive control oligonucleotides provided in the kit display false positives when no template is added. Contamination can occur if you are pipetting high copies (10^6 /µl or above) of template in the same area as carrying out RPA testing. We strongly recommend

I keep getting false positive results, what is the likely cause of this? Read More

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