FAQs

No. Adding more or less rehydration buffer than is recommended can have a detrimental impact on how an RPA reaction works. The buffer contains ingredients that are necessary for RPA reactions, so adding more or less buffer will change their final concentration in a resuspended pellet.

The standard TwistAmp® kits are configured to operate in the temperature range of 37°C – 42°C. At higher temperatures the system will be compromised as the enzymes progressively lose full activity. The RPA process itself can, under appropriate conditions, be performed at much lower temperatures, but the supplied formulations of the TwistAmp® kits are optimised for high kinetic rate and