When using TwistAmp® Liquid reactions how might I improve reproducibility/amplification of my RNA target?

If you are combining your RNA template with MgOAc before adding it to your reactions, you may be stabilising RNA tertiary structures that inhibit the reverse transcriptase. Adding the RNA to your reaction mixture either before you add the MgOAc, or spatially separating it (i.e. adding RNA and MgOAc to opposite sides of your reaction vessel or lid) and adding it simultaneously may improve performance.

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