出版物

每年有越来越多的科学家发现 RPA 确实有效。随着人们不断发现 RPA 的优势,目前已有超过 350 份同行评审出版物*。请从本页的出版物中寻找灵感,思考如何利用 RPA 开发出 PCR 不能实现的试验。
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  • Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus

    Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus

    Author: Feng XY, Shen LB, Wang WZ, Wang JG, Cao ZY, Feng CL, Zhao TT, Zhang SZ.
    The RT-RPA assay showed the same results as those of RT-PCR assay, indicating that the former was highly reliable for SCYLV detection. Analysis of the temperature and time limits revealed a wide operating temperature range from 27 to 45 °C, ...
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  • Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification

    Author: Wang J, Wang J, Zhang R, Liu L, Shi R, Han Q, Yuan W.
    The assay performance was evaluated by testing 76 clinical samples by RT-RPA and a real-time RT-PCR. Fourteen samples were TGEV RNA positive in RT-RPA (18.4%, 14/76), which were also positive in the real-time RT-PCR. The diagnostic agreement between the two ...
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  • Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test

    Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test

    Author: Qi Y, Yin Q, Shao Y, Cao M, Li S, Chen H, Shen W, Rao J, Li J, Li X, Sun Y, Lin Y, Deng Y, Zeng W, Zheng S, Liu S, Li Y.
    The RPA–LF method could differentiate O. tsutsugamushi from other phylogenetically related bacteria. The sensitivity was 100% and specificity was over 90%, when evaluated using infected animal samples or simulative clinical samples. Furthermore, the method was completed in 20 min at ...
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  • Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Listeria monocytogenes Detection in Food.

    Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S.
    Experiments confirmed a detection limit as low as 300 fg of DNA and 1.5 × 101 CFU in pure cultures. Furthermore, RPA‐LF exhibited no cross‐reactions with pathogens. Evaluation of the method with food samples indicated that the detection limit was ...
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  • Detection of Entamoeba Species A Comparative Analysis of Nested Multiplex PCR and Recombinase Polymerase Amplification

    Detection of Entamoeba Species: A Comparative Analysis of Nested Multiplex PCR and Recombinase Polymerase Amplification

    Author: Philips SA, Manochitra K, Padukone S, Chandra S. Parija.
    The aim of the present study was to compare the diagnostic ability of nested-multiplex PCR and Recombinase polymerase amplification for differential detection of E. histolytica and E. dispar. The results of this study showed good agreement between the two tests. ...
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  •  An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus

    An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus.

    Author: Kumar PV, Sharma SK, Rishi N, Ghosh DK, Baranwal VK.
    The results from the present study indicated that RPA assay can be used easily in routine indexing of citrus planting material. To the best of our knowledge, this is the first report on development of a rapid and simplified isothermal ...
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  • Rapid Detection of Staphylococcus aureus via Recombinase Polymerase Amplification Combined with Lateral Flow Strip

    Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S.
    Under optimal conditions of 10 min at 40 °C for RPA, followed by 5 min at room temperature for LF, this method could detect 500 fg genomic DNA and 1.2 × 101 CFU/mL of S. aureus in pure culture. Specificity analysis showed ...
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  • Development of an isothermal amplification-based assay for the rapid detection of Cronobacter spp.

    Development of an isothermal amplification-based assay for the rapid detection of Cronobacter spp.

    Author: Liu S, Geng Y, Liu L, Sun X, Shao J, Han B, Wang J, Tan K
    he RPA and real-time RPA assays reduced the analysis time to less than 15 min and the results were as reliable as those of real-time PCR. Taken together, the RPA and real-time RPA assays served as fast, reliable, and sensitive ...
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  • Rapid and sensitive detection of mink circovirus by recombinase polymerase amplification

    Rapid and sensitive detection of mink circovirus by recombinase polymerase amplification

    Author: Ge J, Shi Y, Cui X, Gu S, Zhao L, Chen H.
    In this study, we developed a nested PCR and established a novel recombinase polymerase amplification (RPA) assay for MiCV detection. Sensitivity analysis showed that the detection limit of nested PCR and RPA assay was 101 copies/reaction, and these methods were ...
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  • Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection

    Author: Kim JY, Koo B, Jin CE, Kim MC, Chong YP, Lee S-O, Choi S-H, Kim YS, Woo JH, Shin Y, Kim S-H.
    The clinical sensitivity of iNAD (100%; 95% CI, 83-100) for SFTS was significantly higher than that of real-time PCR (75%; 95% CI, 51-91; P = 0.047), while its clinical specificity (86%; 95% CI, 65-97) was similar to that of real-time ...
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