出版物

每年有越来越多的科学家发现 RPA 确实有效。随着人们不断发现 RPA 的优势,目前已有超过 350 份同行评审出版物*。请从本页的出版物中寻找灵感,思考如何利用 RPA 开发出 PCR 不能实现的试验。
*TwistDx 不对这些出版物的内容或作者负责。

  • A Sample-to-Targeted Gene Analysis Biochip for Nanofluidic Manipulation of Solid-Phase Circulating Tumor Nucleic Acid Amplification in Liquid Biopsies.

    Author: Koo KM, Dey S, Trau M.
    In this study, complex liquid biopsy sample-to-targeted genetic analysis was conducted on a biochip with a 50 copies-detection limit within 30 min. The biochip uniquely integrated electrical lysis and release of cellular targets with minimal processing; nanofluidic manipulation to accelerate ...
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  • Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1.

    Author: Wang H, Hou P, Zhao G, Yu L, Gao Y, He H.
    Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV) is one of the most highly infectious diseases in livestock, and leads to huge economic losses. In this study, a series of serotype-specific reverse transcription recombinase polymerase amplification assays combined with ...
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  • Development of a reverse transcription recombinase polymerase amplification combined with lateral-flow dipstick assay for avian influenza H9N2 HA gene detection.

    Author: Wang Z, Yang P, Zhang Y, Tian K, Bian C, Zhao J.
    Simple, rapid, sensitive and accurate methods suitable for field detection of H9N2 AIVs are crucial to efficiently control virus infection and spread in time. In this study, an isothermal reverse transcription recombinase polymerase amplification with lateral‐flow dipstick (RT‐RPA‐LFD) assay for ...
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  • Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plants

    Author: Zhao C, Sun F, Li X, Lan Y, Du L, Zhou T, Zhou Y.
    Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallén), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method ...
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  • Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.

    Author: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M.
    Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. ...
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  • Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.

    Author: Sun N, Wang W, Wang J, Yao X, Chen F, Li X, Yinglei Y, Chen B.
    Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. The sensitivity of RT-RPA-LFD assays is ...
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  • Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk.

    Author: Hou P, Zhao G, Wang H, He C, He H.
    The aim of this study was to develop a novel isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), for rapid detection of BVDV. The detection limit of this assay was 20 copies per reaction, and ...
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  • Rapid detection of Listonella anguillarum by recombinase polymerase amplification technique.

    Author: JianHu P, LongLiang Q, HaiLong H, ChenYang D, Peng Z, JiLin X.
    In this study, several pairs of primers and an exo-probe targeting the zinc metalloprotease gene (empA) of Listonella anguillarum was designed and a rapid real-time detection method for Listonella anguillarum based on recombinase polymerase amplification (RPA) was established by optimizing ...
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  • Field-deployable viral diagnostics using CRISPR-Cas13 - ZH

    Rapid detection of mcr-1 by recombinase polymerase amplification

    Author: Xu J, Wang X, Yang L, Kan B, Lu X.
    RPA detection based on the mcr-1 gene was successfully applied in our study. The plasmid-mediated mcr-1 gene conferring colistin drug resistance has a strong ability to spread, suggesting the need to further strengthen the detection of this resistance gene in ...
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  • Rapid detection of cyanobacteria by recombinase polymerase amplification combined with lateral flow strips.

    Author: Li J, Wang C, Yu X, Lin H, Hui C, Shuai L, Zhang S.
    Cyanobacteria are one of the major groups of algae causing algal blooms. In this study a rapid method for detecting Cyanobacteria was developed using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. The method developed in ...
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