出版物

每年有越来越多的科学家发现 RPA 确实有效。随着人们不断发现 RPA 的优势,目前已有超过 350 份同行评审出版物*。请从本页的出版物中寻找灵感,思考如何利用 RPA 开发出 PCR 不能实现的试验。
*TwistDx 不对这些出版物的内容或作者负责。

  • Recombinase Polymerase Amplification Assay for Field Detection of Tomato Bacterial Spot Pathogens.

    Author: Strayer-Scherer AL, Jones LB, Paret ML.
    Recombinase polymerase amplification (RPA) is ideal for in-field detection assays because it is an isothermal technique that is rapid and more tolerant to inhibitors compared to PCR. Hence, novel RPA probes and primers were designed to amplify regions of the ...
    了解更多
  • High-specificity Double-stranded DNA Detection with “Humanoid” Molecular Beacon and TALEs.

    Author: Wang Y, Bai LJ, Qu X, Gao Y, Wang J, Li S, Fan L, Wei H, Liu S, Peng Y, Gao Z, Zhu Y, Gao Z, Ning B.
    Owing to the limitation of DNA-specific recognition, improving the sensitivity of bacteria detection without additional culture poses a considerable challenge. Herein, a sensitive, novel, and high-specificity fluorescence detection method was developed using transcription activator-like effectors (TALEs) as the specifically recognized ...
    了解更多
  • Recombinase Polymerase Amplification Combined with Unmodified Gold Nanoparticles for Salmonella Detection in Milk.

    Author: Chen ZG, Zhong HX, Luo H, Zhang RY, Huang JR.
    In this study, isothermal recombinase polymerase amplification (RPA) was combined with unmodified gold nanoparticles (AuNPs) to detect Salmonella in milk. A rapid, effective, visualized, and low-cost RPA-AuNP assay was developed, with a detection limit of 50 CFU for milk samples ...
    了解更多
  • Isothermal DNA amplification combined with lateral flow dipsticks for detection of biothreat agents.

    Author: Zasada AA, Zacharczuk K, Forminska K, Wiatrzyk A, Ziolkowski R, Malinowska E.
    In the presented study three methods of isothermal DNA amplification loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA) and thermophilic helicase-dependent isothermal DNA amplification (tHDA), were compared for detection of highly dangerous pathogens, such as Bacillus anthracis, Francisella tularensis and ...
    了解更多
  • Visual and equipment-free reverse transcription recombinase polymerase amplification method for rapid detection of foot-and-mouth disease virus.

    Author: Liu L, Wang J, ZhangR, Lin M, Shi R, Han Q, Wang J, Yuan W.
    A visible and equipment-free reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip (LFS RT-RPA) was developed to detect the FMDV using primers and LF probe specific for the 3D gene.
    了解更多
  • A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids.

    Author: Yang H, Chen Z, Cao X, Li Z, Stavrakis S, Choo J, deMello AJ, Howes PD, He N.
    An integrated sample-in-digital-answer-out (SIDAO) diagnostic system incorporating DNA extraction and digital recombinase polymerase amplification, which enables rapid and quantitative nucleic acid analysis from bodily fluids within a disposable cartridge.
    了解更多
  • Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.

    Author: Chen J, Wang Y, Liu X, Chen G, Chen X, Chen J, Liu Z, Gong J, Yang G, Lan Q.
    To determine whether propidium monoazide (PMA) can eliminate the influence of DNA extracted from dead cells, a bacterial suspension was treated with PMA before DNA extraction. Findings of RPA assay showed that DNA extracted from dead cells had no fluorescence ...
    了解更多
  • Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus.

    Author: Ma L, Cong F, Zhu Y, Wu M, Xu F, Huang R, Moore RJ, Guo P.
    A reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. The sensitivity of the RT-RPA assay approached 8 copies per reaction, which is equivalent to the sensitivity of RT-qPCR reactions.
    了解更多
  • Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays.

    Author: Singpanomchai N, Akeda Y, Tomono K, Tamaru A, Santanirand P, Ratthawongjirakul P.
    Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, ...
    了解更多
  • A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.

    Author: Zhao G, Wang H, Hou P, Xia X, He H.
    In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA).
    了解更多