出版物

每年有越来越多的科学家发现 RPA 确实有效。随着人们不断发现 RPA 的优势,目前已有超过 350 份同行评审出版物*。请从本页的出版物中寻找灵感,思考如何利用 RPA 开发出 PCR 不能实现的试验。
*TwistDx 不对这些出版物的内容或作者负责。

  • Akkermansia muciniphila as a model case for the development of an improved quantitative RPA microbiome assay.

    Author: Goux H, Chavan D, Crum M, Kourentzi K, Willson RC.
    Removing DNA with anti-DNA antibodies enabled the development of a quantitative RPA microbiome assay capable of determining the relative abundance of the physiologically-important bacterium Akkermansia muciniphila in human feces.
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  • Sensitive and rapid detection of pathogenic bacteria from urine samples using multiplex recombinase polymerase amplification.

    Author: Chen J, Xu Y, Yan H, Zhu Y, Wang L, Zhang Y, Lu Y, Xing W
    The chip-based bacteria detection proposed in this study is a promising tool for sensitive, accurate, and multiplex identification of bacteria in clinical urine samples of UTIs and bacteriuria.
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  • Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification.

    Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.
    Take-all patch, necrotic ring spot, and summer patch are destructive diseases of amenity turfgrasses in temperate climates. LAMP and RPA assays were created for each of the causal agents of the above diseases. Both assays were highly specific to each ...
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  • Fast and sensitive isothermal DNA assay using micro-bead dielectrophoresis for detection of anti-microbial resistance genes.

    Author: Nakano M, Kalsi S, Morgan H.
    Fast and sensitive detection of anti-microbial resistance gene. Combination of isothermal DNA amplification and microbeads-based dielectrophoretic impedance measurement. Amplicon of target gene was attached on microbeads, then, the amplicon-labeled microbeads were detected. Simultaneous amplification and labeling method was proposed. Detection ...
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  • Isothermal amplification using modified primers for rapid electrochemical analysis of coeliac disease associated DQB1*02 HLA allele.

    Author: Al-Madhagi S, Joda H, Jauset-Rubio M, Ortiz M, Katakis I, O'Sullivan CK.
    The genosensor was applied to genomic DNA and real patient and control blood samples for detection of the coeliac disease associated DQB1*02 HLA allele, as a model system, demonstrating the possibility to carry out molecular diagnostics, combining amplification and detection ...
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  • Development of mobile laboratory for viral haemorrhagic fever detection in Africa.

    Author: Weidmann M, Faye O, Faye O, Abd El Wahed A, Patel P, Batejat C, Manugerra JC, Adjami A, Niedrig M, Hufert FT, Sall AA.
    A mobile laboratory transportable on commercial flights was developed to enable local response to viral hemorrhagic fever outbreaks. The developed mobile laboratory successfully enabled local teams to perform rapid diagnostic testing for viral hemorrhagic fever.
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  • Diagnostic accuracy of a prototype rapid chlamydia and gonorrhoea recombinase polymerase amplification assay: a multi-centre cross-sectional pre-clinical evaluation.

    Author: Harding EM, Fuller SS, Chow SLC, Nori AV, Harrison MA, Parker M, Piepenburg O, Forrest MS, Brooks DG, Patel R, Hay P, Fearnley N, Pond MJ, Dunbar JK, Butcher PD, Planche T, Lowndes CM, Sadiq ST.
    This prototype test has excellent performance characteristics, comparable to currently used NAATs, and fulfils several World Health Organization ASSURED criteria. Its rapidity without loss of performance suggests that once further developed and commercialized, this test could positively affect clinical practice ...
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  • microcephaly_1

    Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method.

    Author: Wand NIV, Bonney LC, Watson RJ, Graham V, Hewson R.
    A novel ZIKV real-time reverse transcriptase RPA (RT-RPA) assay capable of detecting a range of different ZIKV strains from a variety of geographical locations. The ZIKV RT-RPA was shown to be highly sensitive, being capable of detecting as few as ...
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  • A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses

    Author: Tian AL, Elsheikha HM, Zhou DH, Wu YD, Chen MX, Wang M, Chen D, Zhang XC, Zhu XQ.
    A recombinase polymerase amplification (RPA) assay combined with lateral flow (LF) strips was developed for the detection of N. caninum. The LF-RPA assay distinguished N. caninum from nine closely related protozoan species. The amplification reaction was completed in about 10 min ...
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  • Development of Molecular Methods to Detect Macrophomina phaseolina from Strawberry Plants and Soil.

    Author: Burkhardt A, Ramon ML, Smith B, Koike ST, Martin FN.
    RPA has been validated on over 200 infected strawberry plants with a diagnostic sensitivity of 93% and a diagnostic specificity of 99%, respectively. Together, this work demonstrates the value of using new approaches to identify loci for detection and provides ...
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