Review papers

  • Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification

    Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification

    Author: Castellanos-Gonzalez A, White A.C, Melby P, Travi B.
    Among isothermal methods, recombinase polymerase amplification (RPA) is sensitive and potentially applicable at POC. We and others have developed RPA ...
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  • Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

    Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

    Author: Mauk MG, Song J, Liu C, Bau HH.
    Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, ...
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  • Infectious Pathogens Meet Point-of-Care Diagnostics

    Infectious Pathogens Meet Point-of-Care Diagnostics

    Author: Zarei M.
    This review covers the developments on POC diagnosis of Zika, Ebola, Dengue, Malaria, HIV, Hepatitis, and Influenza between 2015 and ...
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  • DNA Amplification and Detection Made Simple (Relatively)

    DNA Amplification and Detection Made Simple (Relatively).

    Author: Hoff M
    The researchers tested the sensitivity, specificity, and speed of RPA by using it to amplify three kinds of human DNA, ...
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  • Kim J Figure

    Isothermal DNA amplification in bioanalysis: strategies and applications.

    Author: Kim J, Easley CJ
    Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been ...
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  • Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

    Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

    Author: Craw P, Balachandran W.
    Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of ...
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  • Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics

    Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics.

    Author: de Paz HD, Brotons P, Muñoz-Almagro C.
    Progressive advances in isothermal amplification chemistries, microfluidics and detectors miniaturisation are paving the way for the introduction and use of ...
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  • Library preparation methods for next-generation sequencing:

    Library preparation methods for next-generation sequencing: Tone down the bias

    Author: Van Dijk EL, Jaszczyszyn Y, Thermes C.
    Next-generation sequencing (NGS) has caused a revolution in biology. NGS requires the preparation of libraries in which (fragments of) DNA ...
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  • Isothermal amplified detection of DNA and RNA

    Isothermal amplified detection of DNA and RNA

    Author: Yan L, Zhou J, Zheng Y, Gamson AS, Roembke BT, Nakayama S, Sintim HO.
    This review highlights various methods that can be used for a sensitive detection of nucleic acids without using thermal cycling ...
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  • Bioanalytical applications of isothermal nucleic acid amplification techniques

    Bioanalytical applications of isothermal nucleic acid amplification techniques

    Author: Deng H, Gao Z.
    This review focuses on the isothermal nucleic acid amplification techniques and their applications in bioanalytical chemistry. Starting off from their ...
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