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Read latest blog post...
Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification
Castellanos-Gonzalez A, White A.C, Melby P, Travi B.
Among isothermal methods, recombinase polymerase amplification (RPA) is sensitive and potentially applicable at POC. We and others have developed RPA ...
Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests
Mauk MG, Song J, Liu C, Bau HH.
Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, ...
Infectious Pathogens Meet Point-of-Care Diagnostics
This review covers the developments on POC diagnosis of Zika, Ebola, Dengue, Malaria, HIV, Hepatitis, and Influenza between 2015 and ...
DNA Amplification and Detection Made Simple (Relatively).
The researchers tested the sensitivity, specificity, and speed of RPA by using it to amplify three kinds of human DNA, ...
Isothermal DNA amplification in bioanalysis: strategies and applications.
Kim J, Easley CJ
Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been ...
Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review
Craw P, Balachandran W.
Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of ...
Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics.
de Paz HD, Brotons P, Muñoz-Almagro C.
Progressive advances in isothermal amplification chemistries, microfluidics and detectors miniaturisation are paving the way for the introduction and use of ...
Library preparation methods for next-generation sequencing: Tone down the bias
Van Dijk EL, Jaszczyszyn Y, Thermes C.
Next-generation sequencing (NGS) has caused a revolution in biology. NGS requires the preparation of libraries in which (fragments of) DNA ...
Isothermal amplified detection of DNA and RNA
Yan L, Zhou J, Zheng Y, Gamson AS, Roembke BT, Nakayama S, Sintim HO.
This review highlights various methods that can be used for a sensitive detection of nucleic acids without using thermal cycling ...
Bioanalytical applications of isothermal nucleic acid amplification techniques
Deng H, Gao Z.
This review focuses on the isothermal nucleic acid amplification techniques and their applications in bioanalytical chemistry. Starting off from their ...
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