Review papers

  • DNA Amplification and Detection Made Simple (Relatively)

    DNA Amplification and Detection Made Simple (Relatively).

    Author: Hoff M
    The researchers tested the sensitivity, specificity, and speed of RPA by using it to amplify three kinds of human DNA, ...
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  • Kim J Figure

    Isothermal DNA amplification in bioanalysis: strategies and applications.

    Author: Kim J, Easley CJ
    Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been ...
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  • Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

    Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

    Author: Craw P, Balachandran W.
    Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of ...
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  • Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics

    Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics.

    Author: de Paz HD, Brotons P, Muñoz-Almagro C.
    Progressive advances in isothermal amplification chemistries, microfluidics and detectors miniaturisation are paving the way for the introduction and use of ...
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  • Library preparation methods for next-generation sequencing:

    Library preparation methods for next-generation sequencing: Tone down the bias

    Author: Van Dijk EL, Jaszczyszyn Y, Thermes C.
    Next-generation sequencing (NGS) has caused a revolution in biology. NGS requires the preparation of libraries in which (fragments of) DNA ...
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  • Isothermal amplified detection of DNA and RNA

    Isothermal amplified detection of DNA and RNA

    Author: Yan L, Zhou J, Zheng Y, Gamson AS, Roembke BT, Nakayama S, Sintim HO.
    This review highlights various methods that can be used for a sensitive detection of nucleic acids without using thermal cycling ...
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  • Bioanalytical applications of isothermal nucleic acid amplification techniques

    Bioanalytical applications of isothermal nucleic acid amplification techniques

    Author: Deng H, Gao Z.
    This review focuses on the isothermal nucleic acid amplification techniques and their applications in bioanalytical chemistry. Starting off from their ...
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  • Molecular-based isothermal tests for field diagnosis of malaria

    Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination

    Author: Oriero EC, Jacobs J, Van Geertruyden JP, Nwakanma D, D'Alessandro U.
    The present review describes LAMP and several other isothermal nucleic acid amplification methods, such as thermophilic helicase-dependent amplification, strand displacement ...
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  • Detection and Characterization of Viral Species

    Detection and Characterization of Viral Species/Subspecies Using Isothermal Recombinase Polymerase Amplification (RPA) Assays

    Author: Glais L, Jacquot E
    In this paper, the complete procedures to set up an RPA assay, applied to detection of RNA (Potato virus Y, ...
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  • Recombinase polymerase amplification - James A

    Recombinase polymerase amplification: Emergence as a critical molecular technology for rapid, low-resource diagnostics

    Author: James A, Macdonald J
    Implementation of RPA technology in developing countries is critically required to assess limitations and potentials of the diagnosis of infectious ...
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