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Two Approaches to Enhance the Processivity and Salt Tolerance of Staphylococcus aureus DNA Polymerase.Author: Zhai B, Chow J, Cheng Q.
In this article, two engineering-strategies were carried out to enhance the processivity of the DNA polymerase used in recombinase polymerase amplification (RPA).
Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potatoAuthor: Babujee L, Witherell RA, Mikami K, Aiuchi D, Charkowski AO, Rakotondrafara AM.
Potato virus Y (PVY) is a global challenge for potato production and the leading cause of seed crop downgrading and rejection for certification. Accurate and timely diagnosis is key to effective control of PVY. In this study, an recombinase polymerase …
Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid.Author: Ma Q, Yao J, Yuan S, Liu H, Wei N, Zhang J, Shan W.
For definitive diagnosis of cryptococcal meningitis, Cryptococcus neoformans and/or C. gattii must be identified within cerebral spinal fluid from the patients. In this study, an assay combining recombinase polymerase amplification (RPA) with lateral flow (LF) was developed to detect C.neoformans …
Rapid molecular detection of macrolide resistanceAuthor: Nelson MM, Waldron CL, Bracht JR.
Emerging antimicrobial resistance is a significant threat to human health. In this study, recombinase polymerase amplification (RPA) is used to detect the antimicrobial resistance gene mef(A) from raw lysates without nucleic acid purification
Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis.Author: Zhao G, Hou P, Huan Y, He C, Wang H, He H.
Mycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. …
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification.Author: Kim NY, Oh J, Lee SH, Kim H, Moon JS, Jeong RD.
In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA …
Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka.Author: Gunaratna G, Manamperi A, Bohlken-Fascher S, Wickremasinge R, Gunawardena K, Yapa B, Pathirana N, Pathirana H, de Silva N, Sooriyaarachchi M, Deerasinghe T, Mondal D, Ranasinghe S, Abd El Wahed A.
In this study, a mobile suitcase laboratory applying novel extraction (SpeedXtract) and isothermal amplification and detection (recombinase polymerase amplification assay, RPA) methods were evaluated for the diagnosis of cutaneous leishmaniasis in Sri Lanka.
Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of “Candidatus Liberibacter asiaticus”.Author: Ghosh DK, Kokane SB, Kokane AD, Warghane AJ, Motghare MR, Bhose S, Sharma AK, Reddy MK.
Currently, polymerase chain reaction (PCR) and real time PCR have been the gold standard techniques used for detection of ‘Ca. L. asiaticus’. These diagnostic methods are expensive, require well equipped laboratories, not user-friendly and not suitable for on-site detection of …
Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.Author: Nybond S, Reu P, Rhedin S, Svedberg G, Alfven T, Gantelius J, Svahn HA.
Described in this study is the use of isothermal amplification (RPA) of viral DNA at 37 °C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles.
Real-time recombinase polymerase amplification assay for the rapid and sensitive detection of Campylobacter jejuni in food samples.Author: Geng Y, Liu G, Lui L, Deng Q, Zhao L, Sun XX, Wang J, Zhao B, Wang J.
In this study, a real-time RPA assay was developed so as to achieve the rapid and efficient detection of C. jejuni by targeting the hipO gene. The specificity and sensitivity of real-time RPA was validated and the practical applicability of …