At what temperature should the TwistAmp® kit be used?

The standard TwistAmp® kits are configured to operate in the temperature range of 37°C – 42°C. At higher temperatures the system will be compromised as the enzymes progressively lose full activity. The RPA process itself can, under appropriate conditions, be performed at much lower temperatures, but the supplied formulations of the TwistAmp® kits are optimised for high kinetic rate and not generally compatible with protocols using temperatures below the recommended range. For RT kits, we recommend that users run reactions at 40°C as the reverse transcriptase performs better at slightly higher temperatures.

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