Can I make a master-mix?

Yes. If you wish to set up multiple reactions, you can make a master mix. If you are screening different DNAs, the resuspension buffer, primers and probe if used can all be mixed together and added to freeze dried reactions to resuspend them. Different DNAs can then be added to reactions before they are started with MgAc as usual. If you are performing a primer screen, it is possible to make a master mix with the resuspension buffer, template DNA, probe (if used) and one of the primers. This should be aliquotted into 1.5 ml tubes and the variable primers added. Only once both primers are present should the freeze dried reactions be resuspended or the formation of recombination filaments will be biased towards the first oligonucleotide added.

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