Yes. It is possible to perform more than one amplification reaction simultaneously in the same tube. However, not all primer pair combinations will work equally well with each other in multiplexing and this format therefore requires careful primer design. Note that the total amount (nmols) of oligonucleotide in the reaction should not significantly exceed that stated in the protocol; if more than two amplification primers are used in a single reaction, then the maximum amount of primer has to be divided between all the oligonucleotides present. Monitoring multiple amplification events at the same time might also require different probes, and limitations on both the detection equipment and the availability of compatible fluorophores, have to be borne in mind.