Yes. The measurement of change of fluorescence can also be used as an endpoint read-out. In this case the fluorescence at the start and at the end of the reaction is compared: an increase of fluorescence signifies a successful amplification event.
Yes. The measurement of change of fluorescence can also be used as an endpoint read-out. In this case the fluorescence at the start and at the end of the reaction is compared: an increase of fluorescence signifies a successful amplification event.