Can I use TwistAmp® reactions for the quantification of template?

Yes. The onset time of detectable amplification for a given assay will depend on the amount of starting template material – the more template copies there are to start with, the earlier the detection time will be. However, exploiting this ‘time-based’ quantification demands a careful experimental setup ensuring simultaneous initiation of compared reactions (e.g. through ‘Magnesium start’). The resolution of quantification is aided by a relatively ‘slow’ amplification reaction. Strategies to slow the rate of amplification (including the design of suitable primers) are discussed in the assay design manual .

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