Yes. The crowding agent and proteins in an RPA reaction interfere with normal agarose gel electrophoresis, so you will probably get a smear, rather than a clean band if you do not clean up your reaction.
Yes. The crowding agent and proteins in an RPA reaction interfere with normal agarose gel electrophoresis, so you will probably get a smear, rather than a clean band if you do not clean up your reaction.