How can reproducibility issues be avoided when utilising small TwistAmp® Liquid reaction volumes?

Reproducibility can obviously be a problem when pipetting small volumes of liquid sub-components as variable component ratios could arise. It is advisable to make a master mix to include the Core Reaction Mix, E-mix etc. which should make things more reproducible. If it’s not feasible to make a master mix, then reverse pipetting can also improve reproducibility.

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