The recommended concentration of primers in the TwistAmp® Basic reaction is 480nM each. In the TwistAmp® exo, TwistAmp® fpg, and Twist Amp® nfo kits the recommended concentration of primer is 420nM each. However, the performance of some primer pairs can be improved by slightly varying their amounts in the reaction and a titration strategy (from 200nM to 600nM each) can be employed to identify the optimal concentration conditions for a given primer pair.
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