Will PCR primers work in RPA?

Yes. We have recently confirmed that primers of lengths typical for use in PCR (e.g. 18-23 nucleotides) work just fine in RPA in many cases. Note though that kinetics can be a little slower, so if you wish to take full advantage of the potential of RPA for rapid amplification within 10-15 minutes we recommend using slightly longer primers (as well as shorter amplicons). Also, note that at this time there is no formally established correlation between the elements which dictate performance of an oligonucleotide as a RPA primer on the one hand and as a PCR primer on the other.

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