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Revolutionary DNA Detection

Publications

Every year more and more scientists are finding out that RPA really works. Nearly 50 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection

    Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4.

    Read more...

  • Real-time & label-free ring-resonator monitoring of solid-phase recombinase polymerase amplification

    Highlights

    • Development of a robust and automated solid-phase amplification platform for optical detection of dsDNA targets.

    • Real-time and label-free monitoring of isothermal solid-phase amplification was achieved using ring resonators in combination with recombinase polymerase amplification.

    • The amplification of a 144-bp target was successfully demonstrated with a limit of detection of 7.8-10−13 M (6-105 copies in 50 -L).

    Read more...

  • Development of portable and rapid assay for the detection of emerging avian influenza A (H7N9) virus

    Highlights

    • The mobile suitcase laboratory is easy to use in low resource settings.

    • The Diagnostics-in-a-Suitcase depends on using cold chain independent reagents.

    • Electricity is supplied from a motor vehicle battery or solar panel battery.

    • The H7N9 RT-RPA assays provided results within 10 minutes.

    Read more...

  • Centrifugal step emulsification for absolute quantification of nucleic acids by digital droplet RPA

    The centrifugal microfluidic droplet generation was used to perform the first digital droplet recombinase polymerase amplification (ddRPA). It was used for absolute quantification of Listerias monocytogenes DNA concentration standards with a total analysis time below 30 min – about a quarter of the time it would take for ddPCR.

    Product used: TwistAmp exo+ Listeria

    Read more . . .

  • Development of a Quantitative RPA Assay with an Internal Positive Control

    A video protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

    Read more . . .

  • Solid-phase RPA on microfluidic DVDs

    Standard un-modified DVD discs and commercial drives are used for low-cost DNA detection. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-negatives and false-positives. The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation. The final disc with reaction products was inserted into a DVD player and microarray images were captured and automatically processed.

    Read more . . .

  • Integrated Quantum Dot Barcode Smartphone Optical Device for Wireless Multiplexed Diagnostics

    RPA is combined with quantum dot barcodes to allow multiplex detection of pathogens with a smartphone. RPA boosted the sensitivity of pathogen detection in a situation where PCR was not possible.

    Product used: TwistAmp® Basic

    Read more . . .

  • Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time, RPA.

    Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is responsible for significant economic losses in the shrimp aquaculture industry. Probit analysis of eight independent experimental replicates showed sufficient sensitivity to detect as few as 4 copies of the IHHNV genome within 7 min at 39 °C with 95 % reliability. Therefore, this rapid RPA method has great potential for applications, either in field use or as a point of care diagnostic technique.

    Product used: TwistAmp® exo

    Read more . . .

  • Event-specific Real-time RPA Detection of Transgenic Rice Kefeng 6

    GM crop detection using RPA – takes 10-20 minutes and can be performed in the field

    Read more . . .

  • An RPA assay for analysis of Mycobacterium tuberculosis using a silicon bio-photonic sensor complex.

    RPA is paired with solid-phase silicon biophotonics-based detection sensors to make a “fast, highly sensitive, accurate and cost-effective assay” to detect tuberculosis.

    Read more . . .

  • Bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings

    RPA (Recombinase Polymerase Amplification) combined with a novel bridging flocculation assay for qualitative evaluation of isothermally amplified plant and other pathogen DNA and RNA by naked eye.

    Read more . . .


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