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A Revolution in DNA Detection

Publications

We have enclosed some publications below you may find of interest.

  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Event-specific Real-time RPA Detection of Transgenic Rice Kefeng 6

    GM crop detection using RPA – takes 10-20 minutes and can be performed in the field

    Read more . . .

  • An RPA assay for analysis of Mycobacterium tuberculosis using a silicon bio-photonic sensor complex.

    RPA is paired with solid-phase silicon biophotonics-based detection sensors to make a “fast, highly sensitive, accurate and cost-effective assay” to detect tuberculosis.

    Read more . . .

  • Bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings

    RPA (Recombinase Polymerase Amplification) combined with a novel bridging flocculation assay for qualitative evaluation of isothermally amplified plant and other pathogen DNA and RNA by naked eye.

    Read more . . .

  • Early Detection of the Dengue Virus Using RT-RPA

    The RPA assay detected as few as 10 copies of DENV RNA in <20 minutes. The designed RT-RPA primers and exo-probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without cross-reacting with other arboviruses. The sensitivity and specificity of the RT-RPA assay were comparable to those of the RT-LAMP and qRT-PCR methods. The RT-RPA assay is rapid and simple to perform without the need for costly equipment or a high level of skill.

    Read more . . .

  • Inhibition of RPA by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA.

    Target DNA enrichment using capture oligonucleotides on a lateral-flow strip removes inhibitors that decrease RPA assay sensitivity.

    Read more . . .

  • Recombinase Polymerase Amplification-Based Assay to Diagnose Giardia in Stool Samples

    An RPA assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. Testing with the assay in the highlands of Peru on 104 stool samples collected from the surrounding communities suggested that further improvements in clinical sensitivity will be needed however.

    Read more . . .

  • Influence of sequence mismatches on the specificity of RPA technology

    A brief investigation of the impact of location and number of template/oligonucleotide mismatches on RPA based on assays developed for tuf gene sequence of Pseudomonas aeruginosa and/or Mycobacterium tuberculosis and for the cfb gene sequence of Streptococcus agalactiae .

    Read more . . . 

  • Isothermal DNA amplification strategies for duplex microorganism detection.

    Detection of Salmonella spp. and Cronobacter spp. in milk by RPA on a biosensor based on DVD technology.

    Read more . . . 

  • Equipment-free incubation of recombinase polymerase amplification reactions using body heat.

    A demonstration of how RPA can be used to amplify and detect as few as 10 copies of  HIV-1 DNA using only the temperature generated by somebody’s armpit.

    Read more . . . 

  • Real-time microfluidic RPA for the toxin B gene of Clostridium difficile on a SlipChip platform

    A miniaturised platform for pathogen detection using the SlipChip concept is demonstrated with an RPA assay for C. difficile. The platform has potential for use in resource-limited environments or at the point-of-care because of its ease of use and low cost, particularly if combined with preserved reagents.

    Read more . . . 

  • RPA of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops

    RPA is used to detect the regulatory sequence of the cauliflower mosaic virus 35S (CaMV-35S) promoter and the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator, which are widely incorporated in genetically modified (GM) crops. The assay could reliably detect as few as 100 copies of the target molecule in a sample within 15–25 min. Furthermore, the real-time RPA detection method was successfully used to amplify and detect DNA from samples of four major GM crops (maize, rice, cotton, and soybean). With this novel amplification method, the test time was significantly shortened and the reaction process was simplified; thus, this method represents an effective approach to the rapid detection of GM crops.

    Read more . . .


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