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Revolutionary DNA Detection


Every year more and more scientists are finding out that RPA really works. Nearly 50 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.

Quantity of publications produced about RPA


    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( ).

    Read more . . .

  • Development of a Quantitative RPA Assay with an Internal Positive Control

    A video protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

    Read more . . .

  • Solid-phase RPA on micruofluidic DVDs

    Standard un-modified DVD discs and commercial drives are used for low-cost DNA detection. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-negatives and false-positives. The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation. The final disc with reaction products was inserted into a DVD player and microarray images were captured and automatically processed.

    Read more . . .

  • Integrated Quantum Dot Barcode Smartphone Optical Device for Wireless Multiplexed Diagnostics

    RPA is combined with quantum dot barcodes to allow multiplex detection of pathogens with a smartphone. RPA boosted the sensitivity of pathogen detection in a situation where PCR was not possible.

    Product used: TwistAmp® Basic

    Read more . . .

  • Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time, RPA.

    Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is responsible for significant economic losses in the shrimp aquaculture industry. Probit analysis of eight independent experimental replicates showed sufficient sensitivity to detect as few as 4 copies of the IHHNV genome within 7 min at 39 °C with 95 % reliability. Therefore, this rapid RPA method has great potential for applications, either in field use or as a point of care diagnostic technique.

    Product used: TwistAmp® exo

    Read more . . .

  • Event-specific Real-time RPA Detection of Transgenic Rice Kefeng 6

    GM crop detection using RPA – takes 10-20 minutes and can be performed in the field

    Read more . . .

  • An RPA assay for analysis of Mycobacterium tuberculosis using a silicon bio-photonic sensor complex.

    RPA is paired with solid-phase silicon biophotonics-based detection sensors to make a “fast, highly sensitive, accurate and cost-effective assay” to detect tuberculosis.

    Read more . . .

  • Bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings

    RPA (Recombinase Polymerase Amplification) combined with a novel bridging flocculation assay for qualitative evaluation of isothermally amplified plant and other pathogen DNA and RNA by naked eye.

    Read more . . .

  • Early Detection of the Dengue Virus Using RT-RPA

    The RPA assay detected as few as 10 copies of DENV RNA in <20 minutes. The designed RT-RPA primers and exo-probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without cross-reacting with other arboviruses. The sensitivity and specificity of the RT-RPA assay were comparable to those of the RT-LAMP and qRT-PCR methods. The RT-RPA assay is rapid and simple to perform without the need for costly equipment or a high level of skill.

    Read more . . .

  • Inhibition of RPA by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA.

    Target DNA enrichment using capture oligonucleotides on a lateral-flow strip removes inhibitors that decrease RPA assay sensitivity.

    Read more . . .

  • Recombinase Polymerase Amplification-Based Assay to Diagnose Giardia in Stool Samples

    An RPA assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. Testing with the assay in the highlands of Peru on 104 stool samples collected from the surrounding communities suggested that further improvements in clinical sensitivity will be needed however.

    Read more . . .

  • Influence of sequence mismatches on the specificity of RPA technology

    A brief investigation of the impact of location and number of template/oligonucleotide mismatches on RPA based on assays developed for tuf gene sequence of Pseudomonas aeruginosa and/or Mycobacterium tuberculosis and for the cfb gene sequence of Streptococcus agalactiae .

    Read more . . . 

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