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Publications

Every year more and more scientists are finding out that RPA really works. Over 80 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • RPA combined with a lateral flow dipstick for rapid and visual detection of Schistosoma japonicum

    Kui Sun, Weiwei Xing, Xinling Yu, Wenliang Fu, Yuanyuan Wang, Minji Zou, Zhihong Luo and Donggang Xu

    From abstract
    With the continuous decline in prevalence and intensity of Schistosoma japonicum infection in China, more accurate and sensitive methods suitable for field detection become much needed for schistosomiasis control. The LFD-RPA assay showed 92.68 % sensitivity, 100 % specificity and excellent diagnostic agreement with the gold standard Kato-Katz test (k = 0.947, Z = 6.36, P < 0.001).

    Product used: TwistAmp® exo

    Read more...

  • Rapid detection of porcine reproductive and respiratory syndrome virus by a fluorescent probe RPA

    Yang Yang, Xiaodong Qin, Yingjun Sun, Ting Chen, Zhidong Zhang

    From abstract
    A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction.

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  • Rapid Detection of Cyprinid Herpesvirus 3 in Latently Infected Koi by RPA

    Meagan A. Prescott, Aimee N. Reed, Ling Jin & Manoj K. Pastey

    From abstract
    Since the emergence of cyprinid herpesvirus 3 (CyHV-3), outbreaks have been devastating to Common Carp Cyprinus carpio and koi (a variant of CommonCarp), leading to high economic losses.. Here we describe the detection of CyHV-3 by recombinase polymerase amplification (RPA). The RPA assay can detect as low as 10 copies of the CyHV-3 genome by an isothermal reaction and yields results in approximately 20 min.

    Product used: TwistAmp® Basic

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  • 역전사 실시간 Recombinase Polymerase Amplification (RT/RT RPA)에 의한 꿀벌 Black Queen Cell Virus의 신속 검출

    (Rapid Detection of Black Queen Cell Virus from Honeybee using Reverse Transcription Real-Time Recombinase Polymerase Amplification (RT/RT RPA))

    임수진, Giang Thi Huong Luong, 민상현, 왕지희, 윤병수

    From abstract

    BQCV-specific DNA amplification could be detected from 3 min 26 sec after RPA reaction with specific DNA templates by RT-RPA, while 41 min 42 sec was required by qRTPCR with same quantities of initial templates.

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  • Simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion

    Kevin M. Koo, Eugene J. H. Wee, Paul N. Mainwaring & Matt Trau

    From abstract
    We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment.

    Product used: TwistAmp® Basic

    Read more..

  • Rapid detection of Porcine circovirus-2 by recombinase polymerase amplification

    Jianchang Wang, Jinfeng Wang, Libing Liu, Ruiwen Li, Wanzhe Yuan

    From abstract
    Porcine circovirus–associated disease, caused primarily by Porcine circovirus-2 (PCV-2), has become endemic in many pig-producing countries and has resulted in significant economic losses to the swine industry worldwide. Tests for PCV-2 infection include PCR, nested PCR, competitive PCR, and real-time PCR (rtPCR). Recombinase polymerase amplification (RPA) has emerged as an isothermal gene amplification technology for the molecular detection of infectious disease agents.

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  • A Novel Isothermal Assay of Borrelia burgdorferi by Recombinase Polymerase Amplification with LF

    Wei Liu, Hui-Xin Liu, Lin Zhang, Xue-Xia Hou, Kang-Lin Wan and Qin Hao

    From abstract
    A novel isothermal detection for recombinase polymerase amplification with lateral flow (LF-RPA) was established for Borrelia burgdorferi detection in this study. This assay with high sensitivity and specificity can get a visible result without any additional equipment in 30 min.

    Product used: TwistAmp® Basic + nfo

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  • Field Demonstration of a Multiplexed Point-of-Care Diagnostic Platform for Plant Pathogens

    Han Yih Lau, Yuling Wang, Eugene J. H. Wee, Jose R. Botella, and Matt Trau

    From abstract
    a rapid, highly specific and sensitive point-of-care method for multiplex detection of plant pathogens was developed by taking advantage of surface-enhanced Raman scattering (SERS) labeled nanotags and recombinase polymerase amplification (RPA), which is a rapid isothermal amplification method with high specificity.

    Product used: TwistAmp® Basic

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  • Development and Evaluation of a Rapid and Sensitive EBOV-RPA Test for Rapid Diagnosis of Ebola Virus

    Mingjuan Yang, Yuehua Ke, Xuesong Wang et al.

    From abstract
    A rapid nucleic acid test based on recombinase polymerase amplification (EBOV-RPA) was developed to specifically detect the 2014 outbreak strains. The EBOV-RPA assay was evaluated by testing samples from suspected EVD patients in parallel with RT-PCR.

    Product used: TwistAmp® exo

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  • Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via RPA

    Kevin M. Koo, Eugene J.H. Wee, Matt Trau

    From abstract

    Herein, we report a novel rapid, cost-efficient and minimal-equipment assay named “FusBLU” for detecting TMPRSS2-ERG gene fusions from urine. TMPRSS2-ERG mRNA was amplified by isothermal reverse transcription-recombinase polymerase amplification (RT-RPA), magnetically-isolated, and detected through horseradish peroxidase (HRP)-catalyzed colorimetric reaction. FusBLU was specific for TMPRSS2-ERG mRNA with a low visual detection limit of 105 copies.

    Product used: TwistAmp® Basic

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  • Centrifugal direct RPA microdevice for multiplex real-time identification of food poisoning bacteria

    Goro Choi,  Jae Hwan Jung, Byung Hyun Park, Seung Jun Oh, Ji Hyun Seo, Jong Seob Choi, Do Hyun Kim and Tae Seok Seo

    From abstract

    In this study, we developed a centrifugal direct recombinase polymerase amplification (direct-RPA) micro device for multiplex and real-time identification of food poisoning bacteria contaminated milk samples. The microdevice was designed to contain identical triplicate functional units and each unit has four reaction chambers, thereby making it possible to perform twelve direct-RPA reactions simultaneously.

    Product used: TwistAmp® exo

    Read more...


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