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Publications

Every year more and more scientists are finding out that RPA really works. Over 80 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Development and Evaluation of a Rapid and Sensitive EBOV-RPA Test for Rapid Diagnosis of Ebola Virus

    Mingjuan Yang, Yuehua Ke, Xuesong Wang et al.

    From abstract
    A rapid nucleic acid test based on recombinase polymerase amplification (EBOV-RPA) was developed to specifically detect the 2014 outbreak strains. The EBOV-RPA assay was evaluated by testing samples from suspected EVD patients in parallel with RT-PCR.

    Product used: TwistAmp® exo

    Read more...

  • Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via RPA

    Kevin M. Koo, Eugene J.H. Wee, Matt Trau

    From abstract

    Herein, we report a novel rapid, cost-efficient and minimal-equipment assay named “FusBLU” for detecting TMPRSS2-ERG gene fusions from urine. TMPRSS2-ERG mRNA was amplified by isothermal reverse transcription-recombinase polymerase amplification (RT-RPA), magnetically-isolated, and detected through horseradish peroxidase (HRP)-catalyzed colorimetric reaction. FusBLU was specific for TMPRSS2-ERG mRNA with a low visual detection limit of 105 copies.

    Product used: TwistAmp® Basic

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  • Centrifugal direct RPA microdevice for multiplex real-time identification of food poisoning bacteria

    Goro Choi,  Jae Hwan Jung, Byung Hyun Park, Seung Jun Oh, Ji Hyun Seo, Jong Seob Choi, Do Hyun Kim and Tae Seok Seo

    From abstract

    In this study, we developed a centrifugal direct recombinase polymerase amplification (direct-RPA) micro device for multiplex and real-time identification of food poisoning bacteria contaminated milk samples. The microdevice was designed to contain identical triplicate functional units and each unit has four reaction chambers, thereby making it possible to perform twelve direct-RPA reactions simultaneously.

    Product used: TwistAmp® exo

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  • RPA combined with lateral flow strip for equipment-free detection of Cryptosporidium spp. oocysts

    Yao-Dong Wu, Dong-Hui Zhou, Long-Xian Zhang, Wen-Bin Zheng, Jian-gang Ma, Meng Wang, Xing-Quan Zhu, Min-Jun Xu

    From abstract

    An RPA test that detects 0.5 oocyst per reaction, with no cross reactivity to other common protozoan species in the intestine of dairy cattle. A simple, rapid, and cost-effective test, with the potential for further development as a diagnostic kit for the diagnosis of cryptosporidiosis of dairy cattle.

    Product used: TwistAmp® nfo

    Read more...

  • Mobile suitcase laboratory for rapid detection of Leishmania donovani using RPA assay

    Dinesh Mondal, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain, Susanne Böhlken-Fascher, Greg Matlashewski, Axel Kroeger, Piero Olliaro and Ahmed Abd El Wahed

    From abstract

    An RPA test for Leishmania donovani was successfully tested using a suitcase laboratory in a hospital in Bangladesh. Forty-eight positive and 48 negative samples showed 100% correlation with PCR results.

    Product used: TwistAmp® exo

    Read more...

  • A Novel Technique to Detect EGFR Mutations in Lung Cancer

    Yuanbin Liu, Ting Lei, Zhiyu Liu, Yanbin Kuang, Jianxin Lyu and Qi Wang

    From abstract

    This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as the AS-RPA-PNA-SYBR (ARPS) system.

    Product used: TwistAmp® Basic

    Read more...

  • Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle

    Benjamin M. C. Swift,  Thomas W. Convery & Catherine E. D. Rees

    From abstract

    A new isothermal DNA amplification protocol using Recombinase Polymerase Amplification (RPA) was developed and was found to be able to detect M. bovis BCG within 48h, with a limit of detection of approximately 10 cells per ml of blood for artificially inoculated blood samples.

    Product used: TwistAmp® Basic

    Read more...

  • Electrochemical detection of Piscirickettsia salmonis genomic DNA from salmon samples

    Jonathan Sabaté del Río, Marketa Svobodova, Paulina Bustos, Pablo Conejeros, Ciara K. O’Sullivan 

    From abstract

    Electrochemical detection of solid-phase RPA of the salmon pathogen Piscirickettsia salmonis in salmon genomic DNA. The system was applied to the analysis of eight real salmon samples, and the method was also compared to qPCR analysis, observing an excellent degree of correlation.

    Product used: TwistAmp® Basic

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  • Rapid Point-of-Use DNA Test for Screening of Genuity® Roundup Ready 2 Yield® Soyabean in Seed Sample

    Dilip Chandu, Sudakshina Paul, Mathew Parker,  Yelena Dudin,  Jennifer King-Sitzes, Tim Perez, Don W. Mittanck, Manali Shah, Kevin C. Glenn,  and Olaf Piepenburg

    From abstract

    A highly sensitive and specific RPA-based detection system for Genuity Roundup Ready 2 Yield (RR2Y) material in soybean (Glycine max) seed samples applicable in both laboratory and field-type settings.

    Product used: Custom Exo

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  • Modular development of a prototype POC molecular diagnostic platform for STIs

    Manoharanehru Branavan, Ruth E Mackay, Pascal Craw, Angel Naveenathayalan, Jeremy C. Ahern, Tulasi Sivanesan, Chris Hudson, Thomas Stead, Jessica Kremer, Neha Garg, Mark Baker, Syed T Sadiq and Wamadeva Balachandran

    Highlights

    •A low cost, optical, POC molecular diagnostic platform.

    •Sample preparation using a paper membrane.

    •Isothermal amplification using HDA and RPA.

    Product used: TwistAmp® fpg

    Read more...

  • FA mathematical model of recombinase polymerase amplification under continuously stirred conditio

    Clint Moody, Heather Newell, Hendrik Viljoen

    From abstract

    Recombinase Polymerase Amplification (RPA), combines this advantage of isothermal PCR with simplicity and rapid amplification. A mathematical model is presented of Recombinase Polymerase Amplification (RPA) and compared to experimental data. This model identifies the rate limiting steps in the chemical process, the effects of stirring, and insights in to using RPA for quantitative measurement of initial DNA concentration. 

    Read more...


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