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Publications

Every year more and more scientists are finding out that RPA really works. Over 80 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus

    Yang Yang,  Xiaodong Qin,  Guangxiang Wang,  Jiaxin Jin,  Youjun Shang and  Zhidong Zhang

    From abstract

    A novel “point of care” molecular amplification assay for rapid visual detection of ORFV was developed based on isothermoal recombinase polymerase amplification (RPA) technology in combination with a simpler lateral flow immunoassay strip (ORFV RPA- LFD assay).

    Product used: TwistAmp® nfo

    Read more...

  • Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care

    Lillis L, Siverson J, Lee A, Cantera J, Parker M, Piepenburg O, Lehman DA, Boyle DS

    From abstract

    Recombinase Polymerase Amplification (RPA) can be used to detect pathogen-specific DNA or RNA in under 20 min without the need for complex instrumentation. These properties enable its potential use in resource limited settings. However, there are concerns that deviations from the manufacturer's protocol and/or storage conditions could influence its performance in low resource settings.

    Product used: TwistAmp® nfo and TwistAmp® exo

    Read more...

  • Evaluation of recombinase polymerase amplification for detection of begomoviruses

    Maria A. Londoño, Carrie L. Harmon and Jane E. Polston

    From abstract

    Recombinase polymerase amplification (RPA) assays for the detection of Bean golden yellow mosaic virus,Tomato mottle virus and Tomato yellow leaf curl virus (TYLCV) were specific, only amplifying the target viruses in three different host species. RPA was able to detect the target virus when the template was in a crude extract generated using a simple inexpensive extraction method, while PCR was not.

    Product used: TwistAmp® Basic

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  • Simple & rapid sample prep system for molecular detection of antibiotic resistant pathogens in urine

    Valiadi M,  Kalsi S, Jones IG, Turner C, Sutton JM, Morgan H

    A new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 min using a custom, valve-less microfluidic chip. Eight samples were processed in parallel, and DNA was released using heat lysis from an integrated resistive heater.

    Product used: TwistAmp® exo

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  • End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

    Gregory Linshiz, Erik Jensen, Nina Stawski, Changhao Bi, Nick Elsbree, Hong Jiao, Jungkyu Kim, Richard Mathies, Jay D. Keasling and Nathan J. Hillson

    From abstract

    RPA is used as part of a programmable, multipurpose microfluidic platform that performs the major steps of the synthetic biology research cycle: design, construction, testing, and analysis. The platform enables the automated control of cellular growth, gene expression induction, and proteogenic and metabolic output analysis.

    Product used: TwistAmp® Basic

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  • Cross-subtype Detection of HIV-1 Using Reverse Transcription and RPA

    Lillis L, Lehman DA, Siverson JB, Weis J, Cantera J, Parker M, Piepenburg O, Overbaugh J, Boyle DS

    From abstract

    In this study we added reverse transcription (RT) to RPA to allow detection of both HIV-1 RNA and DNA. We show that this RT-RPA HIV-1 assay has a limit of detection of 10 to 30 copies of an exact sequence matched DNA or RNA, respectively.
     

    Product used: TwistAmp® exo RT, custom nfo RT

    Read more...

  • Development of a Rapid RPA Assay for Detection of Brucella in Blood Samples

    Hang Ren, Mingjuan Yang,  Guoxia Zhang, Shiwei Liu, Xinhui Wang, Yuehua Ke, Xinying Du, Zhoujia Wang, Liuyu Huang, Chao Liu, Zeliang Chen

    Highlights

    • A rapid and sensitive Bruce-RPA assay was developed for detection of Brucella.

    • The assay could detect as few as 3 copies of Brucella per reaction within 20 min.

    • It represents a candidate point-of-care diagnosis assay for human brucellosis.

    Read more...

  • Rapid Detection of Salmonella Enterica Serovar Enteritidis from Eggs & Chicken Meat by Real-Time RPA

    Ji Yeun Kim and Jung-Lim Lee

    From abstract

    A rapid, sensitive and inexpensive method for the detection of S. Enteritidis from eggs and chicken meat using the real-time recombinase polymerase amplification (Real-time RPA) and compared this assay with the two-step real-time polymerase chain reaction (PCR) (Real-time PCR). The detection sensitivity of the RPA and PCR assays was identical in pure culture, however, the reaction time was as short as 10 min compared with 40 min for the PCR assay. 

    Product used: TwistAmp® exo

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  • Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

    Wang J, Liu L, Li R, Wang J, Fu Q, Yuan W

    From abstract

    A novel RPA-based method for detection of canine parvovirus type 2 (CPV-2) was developed. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings. 

    Product used: TwistAmp® Basic

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  • Naked-Eye Colorimetric and Electrochemical Detection of Mycobacterium tuberculosis

    From abstract:

    Our assays are inexpensive (US$3), rapid (75 min), sensitive (approaching single cell), and highly specific to M. tuberculosis. We believe that our assay could potentially enable public health officials to quickly make important decisions related to the appropriate channeling of precious healthcare resources and form part of the solution to reduce lengthy time-to-diagnosis that is commonly associated with conventional approaches in these settings.

    Product used: TwistAmp Basic

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  • Paper & plastic device for combined isothermal amplification & lateral flow detection of Plasmodium

    From abstract:

    Novel RPA primers were developed that bind to sequences present in the four species of Plasmodium which infect humans. The paper and plastic devices were found to be capable of detecting as few as 5 copies/µL of synthetic Plasmodium DNA (50 copies total), comparable to the same reaction run on the bench top. The devices produce visual results in an hour, cost approximately $1, and are self-contained once the device is sealed

    Product used: TwistAmp nfo

    Read more...


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