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Publications

Every year more and more scientists are finding out that RPA really works. Over 80 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • FA mathematical model of recombinase polymerase amplification under continuously stirred conditio

    Clint Moody, Heather Newell, Hendrik Viljoen

    From abstract

    Recombinase Polymerase Amplification (RPA), combines this advantage of isothermal PCR with simplicity and rapid amplification. A mathematical model is presented of Recombinase Polymerase Amplification (RPA) and compared to experimental data. This model identifies the rate limiting steps in the chemical process, the effects of stirring, and insights in to using RPA for quantitative measurement of initial DNA concentration. 

    Read more...

  • An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp.

    Omar A. Saldarriaga,Alejandro Castellanos-Gonzalez,Renato Porrozzi,Gerald C. Baldeviano,Andrés G. Lescano,Maxy B. de Los Santos,Olga L. Fernandez,Nancy G. Saravia,Erika Costa,Peter C. Melby,Bruno L. Travi 

    A novel molecular method (RPA-LF) that could be applied in the field because it does not require sophisticated equipment. It is also very sensitive and specific to detect the principal Leishmania species that produce cutaneous leishmaniasis in Latin America. Future field implementation of RPA-LF could have a positive impact on disease management and control.

    Product used: TwistAmp® nfo

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  • Picoliter Well Array Chip-Based Digital RPA for Absolute Quantification of Nucleic Acids

    Zhao Li, Yong Liu,Qingquan Wei, Yuanjie Liu,Wenwen Liu,Xuelian Zhang,Yude Yu

    From abstract

    dRPA-on-chip processing required less than 30 min, which was a 4-fold decrease compared to dPCR, requiring approximately 2 h. dRPA on the PWA chip provides a simple and highly sensitive method to quantify nucleic acids without thermal cycling or precise micropump/microvalve control. It has applications in fast field analysis and critical clinical diagnostics under resource-limited settings.

    Product used: TwistAmp® exo

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  • Two-stage sample-to-answer system based on nucleic acid amplification approach for malaria detection

    Qing Liu, Jeonghun Nam, Sangho Kim,Chwee Teck Lim, Mi Kyoung Park, Yong Shin

     

    Highlights

    •A two-stage sample-to-answer system capable of detecting malaria parasite.

    •Rapid, highly sensitive, accurate, and cost-effective system.

    •First combination system of a disposable extraction chip and a real-time sensor.

    •The system would be of great potential for better diagnosis of malaria in real-setting.

    Product used: TwistAmp® Basic

    Read more...

  • Clinical Validation of Quantum Dot Barcode Diagnostic Technology

    Jisung Kim, Mia J. Biondi, Jordan J. Feld and Warren C. W. Chan

    From abstract

    A full detailed clinical validation of Quantum Dot (QD) barcode technology for diagnosing patients infected with Hepatitis B Virus (HBV). We further demonstrate that the detection of multiple regions of the viral genome using multiplexed QD barcodes improved clinical sensitivity from 54.9-66.7% to 80.4-90.5%, and describe how to use QD barcodes for optimal clinical diagnosis of patients.

    Product used: TwistAmp® Basic

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  • Simple Isothermal Strategy for Multiplexed, Rapid, Sensitive, and Accurate miRNA Detection

    Eugene J. H. Wee and Matt Trau

    From abstract

    Herein, we describe miRPA: a novel combination of recombinase polymerase amplification (RPA) with PBCV-1 DNA ligase for simple, specific, rapid, and multiplexed isothermal miRNA detection. This is the first application of RPA for rapid isothermal miRNA detection, and it could have wide applications as a miRNA sensor in both research and in the clinic.

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  • Development of an isothermal amplification-based assay for rapid visual detection of an Orf virus

    Yang Yang,  Xiaodong Qin,  Guangxiang Wang,  Jiaxin Jin,  Youjun Shang and  Zhidong Zhang

    From abstract

    A novel “point of care” molecular amplification assay for rapid visual detection of ORFV was developed based on isothermal recombinase polymerase amplification (RPA) technology in combination with a simpler lateral flow immunoassay strip (ORFV RPA- LFD assay).

    Product used: TwistAmp® nfo

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  • Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care

    Lillis L, Siverson J, Lee A, Cantera J, Parker M, Piepenburg O, Lehman DA, Boyle DS

    From abstract

    Recombinase Polymerase Amplification (RPA) can be used to detect pathogen-specific DNA or RNA in under 20 min without the need for complex instrumentation. These properties enable its potential use in resource limited settings. However, there are concerns that deviations from the manufacturer's protocol and/or storage conditions could influence its performance in low resource settings.

    Product used: TwistAmp® nfo and TwistAmp® exo

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  • Evaluation of recombinase polymerase amplification for detection of begomoviruses

    Maria A. Londoño, Carrie L. Harmon and Jane E. Polston

    From abstract

    Recombinase polymerase amplification (RPA) assays for the detection of Bean golden yellow mosaic virus,Tomato mottle virus and Tomato yellow leaf curl virus (TYLCV) were specific, only amplifying the target viruses in three different host species. RPA was able to detect the target virus when the template was in a crude extract generated using a simple inexpensive extraction method, while PCR was not.

    Product used: TwistAmp® Basic

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  • Simple & rapid sample prep system for molecular detection of antibiotic resistant pathogens in urine

    Valiadi M,  Kalsi S, Jones IG, Turner C, Sutton JM, Morgan H

    A new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 min using a custom, valve-less microfluidic chip. Eight samples were processed in parallel, and DNA was released using heat lysis from an integrated resistive heater.

    Product used: TwistAmp® exo

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  • End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

    Gregory Linshiz, Erik Jensen, Nina Stawski, Changhao Bi, Nick Elsbree, Hong Jiao, Jungkyu Kim, Richard Mathies, Jay D. Keasling and Nathan J. Hillson

    From abstract

    RPA is used as part of a programmable, multipurpose microfluidic platform that performs the major steps of the synthetic biology research cycle: design, construction, testing, and analysis. The platform enables the automated control of cellular growth, gene expression induction, and proteogenic and metabolic output analysis.

    Product used: TwistAmp® Basic

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