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A Revolution in DNA Detection

Publications

We have enclosed some publications below you may find of interest.

  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA Publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • One-pot isothermal DNA amplification – Hybridisation and detection by a disc-based method

    A triplex RPA reaction performed on an integrated sensor comprising isothermal DNA amplification and in situ detection. The method principle combines RPA with detection in the microarray format by compact disc technology as a high-throughput sensing platform.

    Read more . . .

  • Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal RPA Assay

    Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

    Shrimp White Spot Syndrome Virus causes large economic losses to the shrimp aquaculture industry. A newly developed RPA test is more sensitive than previously published qPCR and LAMP tests and delivers results in as little as 7 minutes and has great application potential for field use or point of care diagnostics.

    Read more . . . 

  • Rapid Detection of Mycobacterium tuberculosis by Recombinase Polymerase Amplification

    RPA assays for two Mycobacterium tuberculosis complex specific targets, IS6110 and IS1081, were developed. The tests showed excellent analytical sensitivity and demonstrated superior accuracy to indirect fluorescence microscopy when testing a convenience sample of pulmonary specimens from suspected TB patients.

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  • Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using RPA

    RPA test used to detect plum pox virus with greater sensitivity than ELISA and using crude sample preparation method.

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  • Recombinations in Staphylococcal Cassette Chromosome mec Elements Compromise the Molecular Detection

    Recombinations in Staphylococcal Cassette Chromosome mec Elements Compromise the Molecular Detection of Methicillin Resistance in Staphylococcus aureus.

    A multiplex RPA assay was used to screen 726 MRSA isolates for six common orfX-SCCmec junctions. Next generation sequencing of 24 of the non-detected isolates revealed novel insertions that would require the development of further primers. This result suggests that clinical laboratories cannot rely solely upon molecular assays to reliably detect all methicillin-resistance.

    Read more . . . 

  • Identification of Fungal Pathogens by Visible Microarray System in Combination with Isothermal Gene

    Identification of Fungal Pathogens by Visible Microarray System in Combination with Isothermal Gene Amplification
    Successful identification with PCR-amplified as well as isothermally amplified target genes demonstrated that our microarray system is an efficient and robust method for identifying 42 species from 24 genera of clinically important fungal pathogens in a sample.

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  • Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification

    This paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.

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  • Development of a RPA Assay for the Detection of Pathogenic Leptospira

    Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic Leptospira

    Leptospirosis is a difficult to disease to diagnose. Researchers from KIT Biomedical Research have developed an RPA test that can detect <2 genome copies. The authors believe that “ RPA presents a powerful tool for the early diagnosis of leptospirosis in humans and in animals.”

    Read more . . .

  • Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription- RPA

    Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription - Recombinase Polymerase Amplification (RPA)

    RT-RPA and lateral flow used to detect the Little cherry virus 2 coat protein gene. RT-RPA was simple, fast, and specific. The test has the sensitivity of RT-PCR and is more cost effective, being capable of detecting the virus from crude extracts from field samples.

    Read more . . . 

  • Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using RPA

    Real-Time Isothermal Detection of Shiga Toxin-Producing Escherichia coli Using Recombinase PolymeraseAmplification.
    RPA assays developed to detect Shiga toxin-producing Escherichia coli (STEC), a major family of foodborne pathogens of public health, zoonotic, and economic significance in the United States and worldwide.

    Read more . . . 

  • One-step digital plasma separation for molecular diagnostics

    First demonstration of the coupling of digital plasma separation with RPA nucleic acid detection directly from blood samples.

    Read more . . . 


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