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Publications

Every year more and more scientists are finding out that RPA really works. Over 80 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion

    Kevin M. Koo, Eugene J. H. Wee, Paul N. Mainwaring & Matt Trau

    From abstract
    We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment.

    Product used: TwistAmp® Basic

    Read more..

  • Rapid detection of Porcine circovirus-2 by recombinase polymerase amplification

    Jianchang Wang, Jinfeng Wang, Libing Liu, Ruiwen Li, Wanzhe Yuan

    From abstract
    Porcine circovirus–associated disease, caused primarily by Porcine circovirus-2 (PCV-2), has become endemic in many pig-producing countries and has resulted in significant economic losses to the swine industry worldwide. Tests for PCV-2 infection include PCR, nested PCR, competitive PCR, and real-time PCR (rtPCR). Recombinase polymerase amplification (RPA) has emerged as an isothermal gene amplification technology for the molecular detection of infectious disease agents.

    Read more...

  • A Novel Isothermal Assay of Borrelia burgdorferi by Recombinase Polymerase Amplification with LF

    Wei Liu, Hui-Xin Liu, Lin Zhang, Xue-Xia Hou, Kang-Lin Wan and Qin Hao

    From abstract
    A novel isothermal detection for recombinase polymerase amplification with lateral flow (LF-RPA) was established for Borrelia burgdorferi detection in this study. This assay with high sensitivity and specificity can get a visible result without any additional equipment in 30 min.

    Product used: TwistAmp® Basic + nfo

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  • Field Demonstration of a Multiplexed Point-of-Care Diagnostic Platform for Plant Pathogens

    Han Yih Lau, Yuling Wang, Eugene J. H. Wee, Jose R. Botella, and Matt Trau

    From abstract
    a rapid, highly specific and sensitive point-of-care method for multiplex detection of plant pathogens was developed by taking advantage of surface-enhanced Raman scattering (SERS) labeled nanotags and recombinase polymerase amplification (RPA), which is a rapid isothermal amplification method with high specificity.

    Product used: TwistAmp® Basic

    Read more...

  • Development and Evaluation of a Rapid and Sensitive EBOV-RPA Test for Rapid Diagnosis of Ebola Virus

    Mingjuan Yang, Yuehua Ke, Xuesong Wang et al.

    From abstract
    A rapid nucleic acid test based on recombinase polymerase amplification (EBOV-RPA) was developed to specifically detect the 2014 outbreak strains. The EBOV-RPA assay was evaluated by testing samples from suspected EVD patients in parallel with RT-PCR.

    Product used: TwistAmp® exo

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  • Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via RPA

    Kevin M. Koo, Eugene J.H. Wee, Matt Trau

    From abstract

    Herein, we report a novel rapid, cost-efficient and minimal-equipment assay named “FusBLU” for detecting TMPRSS2-ERG gene fusions from urine. TMPRSS2-ERG mRNA was amplified by isothermal reverse transcription-recombinase polymerase amplification (RT-RPA), magnetically-isolated, and detected through horseradish peroxidase (HRP)-catalyzed colorimetric reaction. FusBLU was specific for TMPRSS2-ERG mRNA with a low visual detection limit of 105 copies.

    Product used: TwistAmp® Basic

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  • Centrifugal direct RPA microdevice for multiplex real-time identification of food poisoning bacteria

    Goro Choi,  Jae Hwan Jung, Byung Hyun Park, Seung Jun Oh, Ji Hyun Seo, Jong Seob Choi, Do Hyun Kim and Tae Seok Seo

    From abstract

    In this study, we developed a centrifugal direct recombinase polymerase amplification (direct-RPA) micro device for multiplex and real-time identification of food poisoning bacteria contaminated milk samples. The microdevice was designed to contain identical triplicate functional units and each unit has four reaction chambers, thereby making it possible to perform twelve direct-RPA reactions simultaneously.

    Product used: TwistAmp® exo

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  • RPA combined with lateral flow strip for equipment-free detection of Cryptosporidium spp. oocysts

    Yao-Dong Wu, Dong-Hui Zhou, Long-Xian Zhang, Wen-Bin Zheng, Jian-gang Ma, Meng Wang, Xing-Quan Zhu, Min-Jun Xu

    From abstract

    An RPA test that detects 0.5 oocyst per reaction, with no cross reactivity to other common protozoan species in the intestine of dairy cattle. A simple, rapid, and cost-effective test, with the potential for further development as a diagnostic kit for the diagnosis of cryptosporidiosis of dairy cattle.

    Product used: TwistAmp® nfo

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  • Mobile suitcase laboratory for rapid detection of Leishmania donovani using RPA assay

    Dinesh Mondal, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain, Susanne Böhlken-Fascher, Greg Matlashewski, Axel Kroeger, Piero Olliaro and Ahmed Abd El Wahed

    From abstract

    An RPA test for Leishmania donovani was successfully tested using a suitcase laboratory in a hospital in Bangladesh. Forty-eight positive and 48 negative samples showed 100% correlation with PCR results.

    Product used: TwistAmp® exo

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  • A Novel Technique to Detect EGFR Mutations in Lung Cancer

    Yuanbin Liu, Ting Lei, Zhiyu Liu, Yanbin Kuang, Jianxin Lyu and Qi Wang

    From abstract

    This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as the AS-RPA-PNA-SYBR (ARPS) system.

    Product used: TwistAmp® Basic

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  • Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle

    Benjamin M. C. Swift,  Thomas W. Convery & Catherine E. D. Rees

    From abstract

    A new isothermal DNA amplification protocol using Recombinase Polymerase Amplification (RPA) was developed and was found to be able to detect M. bovis BCG within 48h, with a limit of detection of approximately 10 cells per ml of blood for artificially inoculated blood samples.

    Product used: TwistAmp® Basic

    Read more...


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