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Publications

Every year more and more scientists are finding out that RPA really works. Over 80 publications have been written in the last 4 years and the rate is going up as people discover the benefits of RPA. See the publications on this page to give you an idea of how you could use RPA for experiements that just aren't possible with PCR.


  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Naked-Eye Colorimetric and Electrochemical Detection of Mycobacterium tuberculosis

    From abstract:

    Our assays are inexpensive (US$3), rapid (75 min), sensitive (approaching single cell), and highly specific to M. tuberculosis. We believe that our assay could potentially enable public health officials to quickly make important decisions related to the appropriate channeling of precious healthcare resources and form part of the solution to reduce lengthy time-to-diagnosis that is commonly associated with conventional approaches in these settings.

    Product used: TwistAmp Basic

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  • Paper & plastic device for combined isothermal amplification & lateral flow detection of Plasmodium

    From abstract:

    Novel RPA primers were developed that bind to sequences present in the four species of Plasmodium which infect humans. The paper and plastic devices were found to be capable of detecting as few as 5 copies/µL of synthetic Plasmodium DNA (50 copies total), comparable to the same reaction run on the bench top. The devices produce visual results in an hour, cost approximately $1, and are self-contained once the device is sealed

    Product used: TwistAmp nfo

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  • On-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis

    From abstract:

    On-chip recombinase polymerase amplification (RPA) on defined spots of a DNA microarray was used to spatially separate the amplification reaction of DNA from two viruses (Human adenovirus 41, Phi X 174) and the bacterium Enterococcus faecalis, which are relevant for water hygiene. Within 48 min the microbes could be identified by the spot position on the microarray while the generated chemiluminescence signal correlated with the amount of applied microbe DNA. 

    Product used: TwistAmp Basic

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  • Development of a fluorescent probe-based RPA assay for rapid detection of Orf virus

    From abstract:

    Orf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. A TwistAmp exo assay was capable of as low as 100 copies of ORFV DNA /reaction and was highly specific, with no cross-reaction with closely related viruses.

    Product used: TwistAmp exo

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  • A multiplexed recombinase polymerase amplification assay to detect intestinal protozoa

    From abstract:

    This paper describes a proof-of-concept multiplex recombinase polymerase amplification (RPA) assay with lateral flow readout that is capable of simultaneously detecting and differentiating DNA from any of the diarrhea-causing protozoa Giardia, Cryptosporidium, and Entamoeba. 

    Product used: TwistAmp nfo

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  • Isothermal solid-phase amplification system for detection of Yersinia pestis.

    Mayboroda O, Benito AG, Del Rio JS, Svobodova M, Julich S, Tomaso H, O'Sullivan CK, Katakis I

    From abstract:

    In this work, RPA was used for the optical detection of solid-phase amplification of the potential bio warfare agent Yersinia pestis. Thiolated forward primers were immobilized on the surface of maleimide-activated microtitre plates for the quantitative detection of synthetic and genomic DNA, with elongation occurring only in the presence of the specific template DNA and solution phase reverse primers.

    Product used: TwistAmp Basic

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  • Development & deployment of rapid RPA Ebola virus detection assay in Guinea in 2015

    O Faye, O Faye, B Soropogui, P Patel, AA El Wahed, C Loucoubar , G Fall , D Kiory, N Magassouba, S Keita, MK Kondé, AA Diallo, L Koivogui , H Karlberg, A Mirazimi, O Nentwich, O Piepenburg, M Niedrig, M Weidmann, AA Sall

    From abstract

    In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on sera and swabs in combination with an improved diagnostic reverse transcription recombinase polymerase amplification assay for the detection of Ebola virus (EBOV-RT-RPA).

    Product used: Custom kit

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  • Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in POC diagnosis

    Benjamin Y.C. Ng, Eugene J.H. Wee, Nicholas P. West, Matt Trau

    From abstract:

    Although there have been many recent advances in Tuberculosis (TB) detection technologies, there still remains a major need to develop simpler point-of-care techniques. In an effort towards such a diagnostic test for resource-poor settings, we have designed a bioassay based on detecting amplified DNA via bridging flocculation. The assay is cheap, with a sensitivity approaching a single cell of Mycobacterium tuberculosis and the potential for translation into broader applications.

    Product used: TwistAmp Basic

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  • Bridging flocculation assay for rapid detection of gene specific DNA methylation

    Eugene J. H. Wee, Thu Ha Ngo and Matt Trau

    From abstract:

    The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples.

    Product used: TwistAmp Basic

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  • Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Gold Nanoparticles

    Benjamin Y. C. Ng, Wei Xiao, Nicholas P. West, Eugene J. H. Wee, Yuling Wang and Matt Trau

    From abstract

    The assay is capable of detecting a positive differential pulse voltammetry (DPV) response from as low as 1 CFU of Mtb bacilli DNA input material, having shown its exquisite sensitivity over a conventional gel based readout. The translation of our assay onto a portable potentiostat was also demonstrated, with promising results. We believe that our assay has significant potential for translation into broader bioassay applications or development as a POC diagnostic tool.

    Product used: TwistAmp Basic

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  • Colorimetric detection of total genomic and loci-specific DNA methylation from limited DNA inputs

    Eugene J. H. Wee, Thu Ha Ngo and Matt Trau

    From abstract

    Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine diagnostics and research.

    Product used: TwistAmp Basic

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