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A Revolution in DNA Detection

Publications

We have enclosed some publications below you may find of interest.

  • DNA DETECTION USING RECOMBINATION PROTEINS

    The original RPA Publication...

    DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited.

    Users interested in using RPA to amplify MRSA DNA should use the oligonucleotides described in our more recent publication ( http://www.ncbi.nlm.nih.gov/pubmed/24972080 ).

    Read more . . .

  • Real-time microfluidic RPA for the toxin B gene of Clostridium difficile on a SlipChip platform

    A miniaturised platform for pathogen detection using the SlipChip concept is demonstrated with an RPA assay for C. difficile. The platform has potential for use in resource-limited environments or at the point-of-care because of its ease of use and low cost, particularly if combined with preserved reagents.

    Read more . . . 

  • RPA of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops

    RPA is used to detect the regulatory sequence of the cauliflower mosaic virus 35S (CaMV-35S) promoter and the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator, which are widely incorporated in genetically modified (GM) crops. The assay could reliably detect as few as 100 copies of the target molecule in a sample within 15–25 min. Furthermore, the real-time RPA detection method was successfully used to amplify and detect DNA from samples of four major GM crops (maize, rice, cotton, and soybean). With this novel amplification method, the test time was significantly shortened and the reaction process was simplified; thus, this method represents an effective approach to the rapid detection of GM crops.

    Read more . . .

  • Non-Instrumented Incubation of a RPA Assay for the Rapid & Sensitive Detection of Proviral HIV-1 DNA

    Development of a prototype cheap, simple, re-usable chemical heater to enable RPA detection of proviral HIV-1 DNA at ambient temperatures of 10-30°C. At ambient temperatures of 31-43°C no heater was required. In both instances 10 copies of proviral HIV-1 DNA could be detected, showing that RPA could be a highly sensitive tool to diagnose infant HIV-1 in Low Resource Settings even without dedicated equipment.

    Read more . . .

  • RPA combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris

    Recombinase Polymerase Amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome.

    A lateral flow-based RPA test has been developed for an important shrimp pathogen, PstDV. The protocol reduced false positive results arising from viral inserts to ∼5% compared to 76%-78% by the IQ2000™ nested PCR kit and the 309F/R PCR protocol currently recommended by World Organization for Animal Health (OIE) for PstDV detection. Together with simplicity and portability, the protocol serves as an alternative tool to PCR for primarily screening PstDV, which is suitable for both laboratory and field application.

    Read more (using TwistAmp® nfo kit) . . .

    Read more (using TwistAmp® Basic kit) . . .

  • One-pot isothermal DNA amplification – Hybridisation and detection by a disc-based method

    A triplex RPA reaction performed on an integrated sensor comprising isothermal DNA amplification and in situ detection. The method principle combines RPA with detection in the microarray format by compact disc technology as a high-throughput sensing platform.

    Read more . . .

  • Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal RPA Assay

    Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

    Shrimp White Spot Syndrome Virus causes large economic losses to the shrimp aquaculture industry. A newly developed RPA test is more sensitive than previously published qPCR and LAMP tests and delivers results in as little as 7 minutes and has great application potential for field use or point of care diagnostics.

    Read more . . . 

  • Rapid Detection of Mycobacterium tuberculosis by Recombinase Polymerase Amplification

    RPA assays for two Mycobacterium tuberculosis complex specific targets, IS6110 and IS1081, were developed. The tests showed excellent analytical sensitivity and demonstrated superior accuracy to indirect fluorescence microscopy when testing a convenience sample of pulmonary specimens from suspected TB patients.

    Read more . . .

  • Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using RPA

    RPA test used to detect plum pox virus with greater sensitivity than ELISA and using crude sample preparation method.

    Read more . . .

  • Recombinations in Staphylococcal Cassette Chromosome mec Elements Compromise the Molecular Detection

    Recombinations in Staphylococcal Cassette Chromosome mec Elements Compromise the Molecular Detection of Methicillin Resistance in Staphylococcus aureus.

    A multiplex RPA assay was used to screen 726 MRSA isolates for six common orfX-SCCmec junctions. Next generation sequencing of 24 of the non-detected isolates revealed novel insertions that would require the development of further primers. This result suggests that clinical laboratories cannot rely solely upon molecular assays to reliably detect all methicillin-resistance.

    Read more . . . 

  • Identification of Fungal Pathogens by Visible Microarray System in Combination with Isothermal Gene

    Identification of Fungal Pathogens by Visible Microarray System in Combination with Isothermal Gene Amplification
    Successful identification with PCR-amplified as well as isothermally amplified target genes demonstrated that our microarray system is an efficient and robust method for identifying 42 species from 24 genera of clinically important fungal pathogens in a sample.

    Read more . . . 

  • Quantification of HIV-1 DNA using Real-Time Recombinase Polymerase Amplification

    This paper describes the use of an internal control and an algorithm to quantify HIV-1 DNA using TwistAmp exo reactions.

    Read more . . . 


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