Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Detection of Entamoeba histolytica by RPA

    Author: Nair G, Rebolledo M, White AC Jr, Crannell Z, Richards-Kortum RR, Pinilla AE, Ramírez JD, López MC, Castellanos-Gonzalez A

    Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was […]

  • Application of Isothermal Amplification Techniques for the Identification of Madurella mycetomatis

    Author: Sarah A. Ahmed, Wendy W. J. van de Sande, Marie Desnos-Ollivier, Ahmed H. Fahal, Najwa A. Mhmoud and G. S. de Hoog

    Both isothermal amplification techniques show high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques is assessed in comparison to conventional PCR using biopsies from eumycetoma patients. RPA […]

  • Colorimetric detection of total genomic and loci-specific DNA methylation from limited DNA inputs

    Author: Eugene J. H. Wee, Thu Ha Ngo and Matt Trau

    Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine […]

  • Bridging flocculation assay for rapid detection of gene specific DNA methylation

    Author: Eugene J. H. Wee, Thu Ha Ngo and Matt Trau

    The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) […]

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