Every year more and more scientists are finding out that RPA really works. Over 170 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Toxoplasma

    Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for detection of Toxoplasma gondii in the environment

    Author: Wu YD, Xu MJ, Wang QQ, Zhou CX, Wang M, Zhu XQ, Zhou DH.
    The amplification product was visualized by the lateral flow (LF) strip within 5 min using the specific probe added to the RPA reaction system. The sensitivity of the established assay was 10 times higher than that of nested PCR with a ...
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  • Napier grass

    Development of field-applicable tests for rapid and sensitive detection of Candidatus Phytoplasma oryzae

    Author: Wambua L, Schneider B, Okwaro A, Wanga JO, Imali O, Wambua PN, Agutu L, Olds C, Jones CS, Masiga D, Midega C, Khan Z, Jores J, Fischer A.
    The assay is based on the amplification of an imp gene fragment, highly specific for this pathogen. Moreover, we demonstrated that this assay can be used on phloem sap directly squeezed from infected plant material and that the pathogen, can be detected ...
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  • RoseRosette_143072020

    A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay.

    Author: Babu B, Washburn BK, Ertekc TS, Miller SH, Riddlea CB, Knox GW, Ochoa-Corona FM, Olsond J, Katırcıoğlue YZ.
    RT-exo RPA analysis of the infected plants using the primer/probe indicated that the virus could be detected from leaves, stems, petals, pollen, primary roots and secondary roots.
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  • Screen Shot 2017-06-09 at 12.04.36

    Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Common Bacterial Urinary Tract Infection Pathogens

    Author: Raja B, Goux HJ, Marapadaga A, Rajagopalan S, Kourentzi K, Willson RC.
    The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterococcus faecalis. All five RPAs required reaction times of under 12 minutes to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with ...
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  • Nanoribbons

    Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors

    Author: Hu C, Kalsi S, Zeimpekis I, Sun K, Ashburn P, Turner C, Sutton JM, Morgan H.
    A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method.
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  • Bananas

    Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars

    Author: Kapoor R, Srivastava N, Kumar S, Saritha RK, Sharma SK, Jain RK, Baranwal VK.
    In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both ...
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  • Potato blight Phytophthora infestans _611753135

    Development of Real-Time Isothermal Amplification Assays for On-Site Detection of Phytophthora infestans in Potato Leaves

    Author: Ammour MS, Bilodeau GJ, Tremblay DM, Van der Heyden H, Yaseen T, Varvaro L, Carisse O.
    The assay’s specificity was tested using several species of Phytophthora and other potato fungal and oomycete pathogens. Both LAMP and RPA assays showed specificity to P. infestans but also to the closely related species P. andina, P. mirabilis, P. phaseoli, and P. ipomoeae, although the latter are not ...
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    Nucleic acid detection with CRISPR-Cas13a/C2c2

    Author: Gootenberg JS, Abudayyeh OO, Wook Lee J, Essletzbichler P, Dy AJ, Joung J, Verdine V, Zhang F, et al
    We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumour DNA.
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  • Porcine Circovirus 2

    Development of a real-time recombinase polymerase amplification assay for rapid and sensitive detection of porcine circovirus 2

    Author: Wang J, Wang J, Liu L, Yuan W.
    The two assays demonstrated a 100% diagnostic agreement, and PCV-2 DNA was detected in 26 samples. The R2 value of real-time RPA and real-time PCR was 0.954 by linear regression analysis. The real-time RPA assay provides an alternative tool for rapid, ...
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  • Ebola_virus

    Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics

    Author: Magro L, Jacquelin B, Escadafal C, Garneret P, Kwasiborski A, Manuguerra C, Monti F, Sakuntabhai A, Vanhomwegen J, Lafaye P, Tabeling P.
    RT-RPA results were available in few minutes and demonstrate a sensitivity of 90.0% compared to the gold-standard RT-PCR on a set of 43 patient samples.
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