Every year more and more scientists are finding out that RPA really works. Over 200 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Rapid DNA detection of Mycobacterium tuberculosis

    Establishment of a rapid and sensitive method based on recombinase polymerase amplification to detect mts90, a new molecular target of Mycobacterium tuberculosis

    Author: Mo Y, Cui F, Li X, Zhang X, Qiu Y, Yin Y, Zhang X, Xu W.
    The assay was specific for detecting MTB, as it did not identify the genomic DNA from other mycobacteria and pathogens.
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  • Grapevine Red Blotch Virus

    Development of a fast AmplifyRP Acceler8 Diagnostic Assay For Grapevine Red Blotch Virus.

    Author: Li R, Fuchs MF, Perry KL, Mekuria T, Zhang S.
    The sensitivity of AmplifyRP Acceler8 for GRBV detection is approximately 100 times higher than that of PCR.
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  • Tick on skin_183149369

    A recombinase polymerase amplification assay for rapid detection of Crimean-Congo Haemorrhagic fever Virus infection

    Author: Bonney LC, Watson RJ, Afrough B, Mullojonova M, Dzhuraeva, Tishkova F, Hewson R.
    The assay is a rapid, isothermal, simple-to-perform molecular diagnostic, which can be performed on a light, portable real-time detection device. It is ideally placed therefore for use as a field-diagnostic or in-low resource laboratories, for monitoring of CCHF outbreaks at ...
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  • Coxiella burnetii Q Fever

    A rapid bio-optical sensor for diagnosing Q fever in clinical specimens

    Author: Koo B, Jin CE, Park SY, Lee TY, Nam J, Jang YR, Kim SM, Kim JY, Kim SH, Shin Y.
    We confirmed the clinical sensitivity (> 90%) of the bio-optical sensor by detecting C. burnetii in 11 formalin-fixed, paraffin-embedded liver biopsy samples from acute Q-fever hepatitis patients.
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  • 1.	Establishment of a Real-time Recombinase Polymerase Amplification Assay for Rapid Detection of African Swine Fever Virus

    A recombinase polymerase amplification-based assay for rapid detection of African swine fever virus.

    Author: Wang J, Wang J, Geng Y, Yuan W.
    Tests of recombinant plasmid-spiked serum samples revealed that RPA and real-time PCR had the same diagnostic rate. The RPA assay, which is simple, cost-effective, and fast, is a promising alternative to real-time PCR for ASFV detection.
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  • Pigs

    Recombinase polymerase amplification assay for rapid detection of porcine circovirus 3

    Author: Wang J, Zhang Y, Zhang R, Han Q, Wang J, Liu L, Li R, Yuan W.
    •A real-time RPA assay based on exo probe was developed for the rapid detection of PCV3. •The assay showed high analytical sensitivity and specificity for PCV3 detection. •Clinical samples collected from 2014 to July 2017 were used for further assay optimization. •Real-time RPA ...
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  • Rabies

    Development of molecular confirmation tools for swift and easy rabies diagnostics

    Author: Schlottau K, Freuling CM, Muller T, Beer M, Hoffmann B.
    Here, novel assays, i.e. HighSpeed RT-qPCR and isothermal recombinase polymerase amplification (RPA) were designed and tested. Furthermore, three magnetic bead-based rapid extraction methods for manual or automated extraction were validated and combined with the new downstream assays.
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  • 1.	Rapid isothermal point mutation detection – towards a first pass screening strategy for multidrug resistant tuberculosis

    Rapid isothermal point mutation detection – towards a first pass screening strategy for multidrug resistant tuberculosis

    Author: Ng BYC, Wee EJH, Woods K, Anderson W, Antaw F, Tsang HZH, West NP, Trau M.
    Herein, we describe a novel strategy that enabled exquisite point mutation discrimination with isothermal DNA amplification, using mismatched primers in conjunction with a two-round enrichment process. As a proof of concept, the method was applied to the rapid and specific ...
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  • Abalone

    Real-time isothermal detection of Abalone herpes-like virus and red-spotted grouper nervous necrosis virus using recombinase polymerase amplification

    Author: Gao F, Jiang J-Z, Wang J-Y, Wei H-Y
    • This is the first study to use RPA to detect AbHV and RGNNV. • Reaction can be finished at 37 °C in 20 min; time can be further reduced to 5 min for high viral load sample. • The detection limits ...
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  • Solid-Phase Isothermal DNA Amplification and Detection on Quartz Crystal Microbalance Using Liposomes and Dissipation Monitoring

    Author: Grammoustianou A, Papadakis G, Gizeli E.
    The presented methodology possesses several advantages over existing methods, i.e., it is fast, achieving the detection of DNA produced after only 5 min of amplification, and simple, since it does not require any post-amplification DNA extraction step.
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