Publications

Every year more and more scientists are finding out that RPA really works. Over 250 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick.

    Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick.

    Author: Wang H, Sun M, Xu D, Podok P, Xie J, Jiang Ys, Lu Lq.
    Herein, a rapid and convenient detection assay based on RPA with a lateral flow dipstick (LFD) was developed for detecting CyHV‐2. The highly conserved ORF72 of CyHV‐2 was targeted by specific and sensitive primers and probes. The optimized assay takes ...
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  • novel method to detect meat adulteration by recombinase polymerase amplification and SYBR green I

    A novel method to detect meat adulteration by recombinase polymerase amplification and SYBR green I

    Author: Cao Y, Zheng K, Jiang J, Wu J, Shi F, Song X, Jiang Y.
    At the isothermal temperature of 37 °C, RPA specifically identifies duck, chicken, cow, sheep and pig within 30 min of water bath. The RPA amplicons were successfully visualized by adding SG I. Furthermore, RPA can differentiate species of boiled, microwaved, high pressured ...
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  • Rapid Authentication of Ginkgo biloba Herbal Products Using the Recombinase Polymerase Amplification Assay

    Rapid Authentication of Ginkgo biloba Herbal Products Using the Recombinase Polymerase Amplification Assay

    Author: Liu Y, Wang XY, Wei XM, Gao ZT, Han JP.
    In this study, we developed a rapid identification method using the recombinase polymerase amplification (RPA) assay to detect two species, Ginkgo biloba and Sophora japonica(as adulteration), in Ginkgo biloba HPs.
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  • Field Detection of Citrus Huanglongbing Associated with Candidatus Liberibacter Asiaticus

    Field Detection of Citrus Huanglongbing Associated with 'Candidatus Liberibacter Asiaticus' by Recombinese Polymerase Amplification within 15 min.

    Author: Qian W, Lu Y, Meng Y, Ye Z, Wang L, Wang R, Zheng Q, Wu J.
    In this paper, an extremely rapid and simple method for field detection of Las from leaf samples, based on recombinase polymerase amplification (RPA), is described. Three RPA primer pairs were designed and evaluated. RPA amplification was optimized so that it ...
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  • Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71

    Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71

    Author: Yin D, Zhu Y, Wang K, Wang J, Zhang X, Han M, He Y, Chen Q, Hu G.
    A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%.
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  • Trypanosoma cruzi Infection in Dogs

    Efficacy of Recombinase Polymerase Amplification to Diagnose Trypanosoma cruzi Infection in Dogs with Cardiac Alterations from an Endemic Area of Mexico

    Author: Jimenez-Coello M, Shelite T, Castellanos-Gonzalez A, Saldarriaga O, Rivero R, Ortega-Pacheco A, Garg N, Acevedo-Arcique C, Amaya-Guardia K, Melby P, Travi BL.
    The analytical sensitivity indicated that RPA-LF amplified T. cruzi DNA in samples containing almost equal to one to two parasites per reaction. Serial twofold dilutions of T. cruzi epimastigotes showed that the test had 95% (19/20) repeatability at concentrations of ...
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  • Semi-quantitative nucleic acid test with simultaneous isotachophoretic extraction and amplification.

    Semi-quantitative nucleic acid test with simultaneous isotachophoretic extraction and amplification.

    Author: Bender AT, Borysiak MD, Levenson AM, Lillis L, Boyle DS, Posner JD.
    We demonstrate simultaneous ITP and RPA can consistently detect 5 copies per reaction in buffer and 10 000 copies per milliliter of human serum with no intermediate user steps. We also show preliminary extraction and amplification of DNA from whole blood ...
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  • Stapphlococus

    Rapid Detection of Staphylococcus aureus in Food Using a Recombinase Polymerase Amplification-Based Assay

    Author: Geng Y, Liu S, Wang J, Nan H, Lui L, Sun X, Li D, Liu M, Wang J, Tan K.
    In the present study, RPA and real-time RPA assays were developed and validated to detect S. aureus with high sensitivity and specificity by targeting the nuc gene for the first time. The analytical sensitivity of real-time RPA was 102 copies/reaction, ...
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  • Canine parovirus

    Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

    Author: Liu L, Wang J, Geng Y, Wang J, Li R, Shi R, Yuan W.
    The assay performance was further evaluated by testing 60 canine fecal samples, and CPV-2 DNA was detected in 46 samples (76.7%, 46/60) by LFS RPA, which was the same result as that of the real-time PCR assay and higher than ...
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  • Field-deployable viral diagnostics using CRISPR-Cas13

    Field-deployable viral diagnostics using CRISPR-Cas13

    Author: Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner MJ,
    In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating ...
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