Every year more and more scientists are finding out that RPA really works. Over 150 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • 216_2017_269_Figa_HTML

    Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

    Author: Jauset-Rubio M, Lobato IM, Mayboroda O, Katakis I, O’Sullivan CK.
    Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio (1:0, 1:1, 1:10 and 1:100) and length ...
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  • 604_2017_2144_Figa_HTML

    Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection

    Author: Yamanaka ES, Tortajada-Genaro LA, Maquieira Á.
    An amplification chip platform containing 100 wells was manufactured with a 3D printer and using thermoplastic polylactic acid. The platform reduces reagent consumption and allows parallelization.
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  • African Cows

    Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions

    Author: Fangyuan Yin, Junlong Liu, Aihong Liu, Youquan Li, Jianxun Luo, Guiquan Guan
    Herein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples.
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  • Schistosoma_31917985_200px

    Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China

    Author: Weiwei Xing, Xinling Yu, Donggang Xu et al
    The S.japonicum RPA assay was developed to target highly repetitive retrotransposon SjR2 gene of S japonicum, and its sensitivity and specificity were assessed by serial dilution of S. japonicum genomic DNA and other related worm genomic DNA respectively.
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  • GA

    A nanoplasmonic label-free surface-enhanced Raman scattering strategy for non-invasive cancer genetic subtyping in patient samples

    Author: Jing Wang, Kevin M. Koo,  Eugene J. H. Wee, Yuling Wang, Matt Trau
    We herein present a novel strategy based on multiplex reverse transcription-recombinase polymerase amplification (RT-RPA) to enrich multiple RNA biomarkers, followed by label-free SERS with multivariate statistical analysis to directly detect, identify and distinguish between these long amplicons (∼200 bp).
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  • Orf virus is a member of the parapoxvirus genus

    Development of real-time and lateral flow strip reverse transcription recombinase polymerase amplification assays for rapid detection of peste des petits ruminants virus

    Author: Yang Yang, Xiaodong Qi, Zhidong Zhang et al
    In this study, reverse transcription recombinase polymerase amplification assays using real-time fluorescent detection (real-time RT-RPA assay) and lateral flow strip detection (LFS RT-RPA assay) were developed targeting the N gene of PPRV.
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  • HPV

    Isothermal Method of a Recombinase Polymerase Amplification Assay for the Detection of Most Common High-Risk Human Papillomavirus Type 16 and Type 18 DNA

    Author: Biao Ma, Jiehong Fang, Mingzhou Zhang et al
    In this study, we evaluate the efficacy of the RPA assay, incubating clinical specimens of HPV16 and HPV18 using plasmids standard. It operates at constant low temperature without the thermal instrumentation for incubation.
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  • 144_Song

    Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection

    Author: Jinzhao Song, Changchun Liu, Michael G. Mauk, Shelley C. Rankin, James B. Lok, Robert M. Greenberg, Haim H. Bau
    To address the need for point-of-care (POC) highly multiplexed tests, we propose the 2-stage, nested-like, rapid (<40 >
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  • Goat 2_282513164

    Development of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection

    Author: Tu PA, Shiu JS, Lee SH, Pang VF, Wang DC, Wang PH.
    Under the optimal incubation conditions, specifically, 30 min at 37 °C for RPA followed by 5 min at room temperature for LFD, the assay was found to be sensitive to a lower limit of 80 pg of total DNA and ...
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  • Microcephaly_1

    Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay

    Author: Abd El Wahed A, Sanabani SS, Faye O, Pessoa R, Patriota, Weidmann M et al
    In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes).
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