Publications

Every year more and more scientists are finding out that RPA really works. Over 200 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Bacillus anthracis (Anthrax)

    Sensitive and specific recombinase polymerase amplification set of assays for fast screening, detection and identification of Bacillus anthracis in a field setting.

    Author: Bentahir M, Ambroise J, Delcorps C, Pilo P, Gala JL.
    RPA tests displayed 100% specificity and sensitivity. The hands-off turnaround time at 42°C ranged from 5 to 6 min for 102 genomic copies. The analytical sensitivity of each RPA was ∼10 molecules per reaction. Besides, BA_5345 and adk RPA assays ...
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  • Tick on skin_183149369

    Simultaneous detection of Theileria annulata and Theileria orientalis infections using recombinase polymerase amplification

    Author: Hassan MA, Liu J, Sajid MS, Rashid M, Mahmood A, Abbas Q, Guan G, Yin H, Luo J.
    The multiplex RPA specifically amplified 282-bp and 229-bp fragments of the enolase gene from T. annulata and T. orientalis and had no cross-reaction with other piroplasm species. It was determined that 45 (23.9%) and 5 (2.6%) out of 188 blood ...
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  • detection of isothermally amplified HOTAIR long non-coding RNA

    Naked-eye and electrochemical detection of isothermally amplified HOTAIR long non-coding RNA

    Author: Islam MN, Moriam S, Umer M, Phan HP, Salomon C, Kline R, Nguyen NT, Shiddiky MJA.
    Herein, we report on the development of a new colorimetric and electrochemical assay platform for long non-coding HOX transcript antisense intergenic RNA (HOTAIR) detection. Isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) was performed to amplify HOTAIR sequences from a RNA pool ...
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  • Washing-Free Electrochemical Detection of Amplified Double-Stranded DNAs Using a Zinc Finger Protein

    Washing-Free Electrochemical Detection of Amplified Double-Stranded DNAs Using a Zinc Finger Protein.

    Author: Fang CS, Kim KS, Ha DT, Kim MS, Yang H.
    The whole detection is performed within 17 min (15 min for the RPA reaction and <2 >
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  • Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus

    Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus

    Author: Feng XY, Shen LB, Wang WZ, Wang JG, Cao ZY, Feng CL, Zhao TT, Zhang SZ.
    The RT-RPA assay showed the same results as those of RT-PCR assay, indicating that the former was highly reliable for SCYLV detection. Analysis of the temperature and time limits revealed a wide operating temperature range from 27 to 45 °C, ...
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  • Pigs

    Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification

    Author: Wang J, Wang J, Zhang R, Liu L, Shi R, Han Q, Yuan W.
    The assay performance was evaluated by testing 76 clinical samples by RT-RPA and a real-time RT-PCR. Fourteen samples were TGEV RNA positive in RT-RPA (18.4%, 14/76), which were also positive in the real-time RT-PCR. The diagnostic agreement between the two ...
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  • Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test

    Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test

    Author: Qi Y, Yin Q, Shao Y, Cao M, Li S, Chen H, Shen W, Rao J, Li J, Li X, Sun Y, Lin Y, Deng Y, Zeng W, Zheng S, Liu S, Li Y.
    The RPA–LF method could differentiate O. tsutsugamushi from other phylogenetically related bacteria. The sensitivity was 100% and specificity was over 90%, when evaluated using infected animal samples or simulative clinical samples. Furthermore, the method was completed in 20 min at ...
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  • Listeria-monocytogenes

    Recombinase Polymerase Amplification Combined with Lateral Flow Strip for Listeria monocytogenes Detection in Food.

    Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S.
    Experiments confirmed a detection limit as low as 300 fg of DNA and 1.5 × 101 CFU in pure cultures. Furthermore, RPA‐LF exhibited no cross‐reactions with pathogens. Evaluation of the method with food samples indicated that the detection limit was ...
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  •  An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus

    An isothermal based recombinase polymerase amplification assay for rapid, sensitive and robust indexing of citrus yellow mosaic virus.

    Author: Kumar PV, Sharma SK, Rishi N, Ghosh DK, Baranwal VK.
    The results from the present study indicated that RPA assay can be used easily in routine indexing of citrus planting material. To the best of our knowledge, this is the first report on development of a rapid and simplified isothermal ...
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  • Stapphlococus

    Rapid Detection of Staphylococcus aureus via Recombinase Polymerase Amplification Combined with Lateral Flow Strip

    Author: Du X-J, Zang Y-X, Liu H-B, Li P, Wang S.
    Under optimal conditions of 10 min at 40 °C for RPA, followed by 5 min at room temperature for LF, this method could detect 500 fg genomic DNA and 1.2 × 101 CFU/mL of S. aureus in pure culture. Specificity analysis showed ...
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