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Publications

Every year more and more scientists are finding out that RPA really works. Over 150 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • SHERLOCK CRISPR

    Nucleic acid detection with CRISPR-Cas13a/C2c2

    Author: Gootenberg JS, Abudayyeh OO, Wook Lee J, Essletzbichler P, Dy AJ, Joung J, Verdine V, Zhang F, et al
    We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumour DNA.
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  • Porcine Circovirus 2

    Development of a real-time recombinase polymerase amplification assay for rapid and sensitive detection of porcine circovirus 2

    Author: Wang J, Wang J, Liu L, Yuan W.
    The two assays demonstrated a 100% diagnostic agreement, and PCV-2 DNA was detected in 26 samples. The R2 value of real-time RPA and real-time PCR was 0.954 by linear regression analysis. The real-time RPA assay provides an alternative tool for rapid, ...
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  • Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification

    Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification

    Author: Wang R, Zhang F, Wang L, Qian W, Qian C, Wu J, Ying Y.
    In this paper, a simple, visual and instrument-free method for instant on-site detection of GTS 40-3-2 soybean has been developed. It is based on body-heat recombinase polymerase amplification (RPA) and followed with naked-eye detection via fluorescent DNA dye.
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  • Porcine circovirus type 2

    Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

    Author: Yang Y, Qin X, Sun Y, Cong G, Li Y, Zhang Z.
    Both assays were highly specific for PCV2, with no cross-reactions with porcine circovirus virus type 1, foot-and-mouth disease virus, pseudorabies virus, porcine parvovirus, porcine reproductive and respiratory syndrome virus, and classical swine fever virus.
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  • Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip

    Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip

    Author: Yeh EC, Fu C-C, Hu L, Thakur R, Feng J, Lee LP.
    We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps.
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  • Breed-specific detection of mangalica meat in food products

    Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus

    Author: Yang Y, Qin X, Zhang W, Li Z, Zhang S, Li Y, Zhang Z.
    Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV).
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  • Aboriginal mitogenomes reveal 50,000 years of regionalism in Australia

    Aboriginal mitogenomes reveal 50,000 years of regionalism in Australia

    Author: Tobler R, Rohrlach A, Soubrier J, Bover P, Llamas B, Tuke J, Bean N, Cooper A et al
    Here we report 111 mitochondrial genomes (mitogenomes) from historical Aboriginal Australian hair samples, whose origins enable us to reconstruct Australian phylogeographic history before European settlement. 
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  • 216_2017_269_Figa_HTML

    Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

    Author: Jauset-Rubio M, Lobato IM, Mayboroda O, Katakis I, O’Sullivan CK.
    Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio (1:0, 1:1, 1:10 and 1:100) and length ...
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  • 604_2017_2144_Figa_HTML

    Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection

    Author: Yamanaka ES, Tortajada-Genaro LA, Maquieira Á.
    An amplification chip platform containing 100 wells was manufactured with a 3D printer and using thermoplastic polylactic acid. The platform reduces reagent consumption and allows parallelization.
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  • African Cows

    Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions

    Author: Fangyuan Yin, Junlong Liu, Aihong Liu, Youquan Li, Jianxun Luo, Guiquan Guan
    Herein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples.
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