Every year more and more scientists are finding out that RPA really works. Over 250 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • 290_Toldra

    Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay.

    Author: Toldra A, Jauset-Rubio M, Andree KB, Fernandez-Tejedor M, Diogene J, Katakis I, O'Sullivan CK, Campas M.
    An assay for the detection of two Karlodinium species has been developed. The assay is based on isothermal DNA amplification and a sandwich hybridisation. The assay discriminates between Karlodinium veneficum and Karlodinium armiger. Seawater samples spiked with Karlodinium cells have ...
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  • 291_Wang

    Rapid detection of foot-and-mouth disease virus using reverse transcription recombinase polymerase amplification combined with a lateral flow dipstick.

    Author: Wang H, Zhao G, Hou P, Yu L, He C, He H.
    The RPA-LFD assay was highly sensitive, detecting down to 10 copies of plasmid DNA. There was no cross-reactivity with other pathogens causing vesicular lesions. In addition, 143 clinical samples were used to compare RPA-LFD with real-time PCR, with 98.6% concordance ...
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  • 289_Hu2

    Rapid and visual detection of Group B streptococcus using recombinase polymerase amplification combined with lateral flow strips.

    Author: Hu S, Zhong H, Huang W, Zhan W, Yang X, Tang B, Chen K, Wang J, Hu T, Zhang C, Zhou Z, Luo M.
    A rapid molecular method for GBS detection was developed and evaluated. This assay has high specificity and sensitivity for diagnosing GBS, which is suitable for clinical application. The assay can be completed within about 30 minutes without the need of ...
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  • 288_Koo

    Design and Clinical Verification of Surface Enhanced Raman Spectroscopy Diagnostic Technology for Individual Cancer Risk Prediction.

    Author: Koo KM, Wang J, Richards RS, Farrell A, Yaxley JW, Samaratunga H, Teloken PE, Roberts MJ, Coughlin GD, Lavin MF, Mainwaring PN, Wang Y, Gardiner RA, Trau M.
    Describes a comprehensive clinical verification of a prospective label-free surface-enhanced Raman scattering (SERS) nanodiagnostic assay for prostate cancer (PCa) risk stratification.
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  • Canine parovirus

    Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus.

    Author: Wang J, Wang J, Li R, Shi R, Liu L, Yuan W.
    Visual, rapid molecular assay for detection of CDV was developed. Assay is based on RT-RPA and use of a lateral flow strip to visualize product. Assay was performed in closed fists using body heat for 15 min. Analytical sensitivity and specificity ...
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  • 283_Goux

    Akkermansia muciniphila as a model case for the development of an improved quantitative RPA microbiome assay.

    Author: Goux H, Chavan D, Crum M, Kourentzi K, Willson RC.
    Removing DNA with anti-DNA antibodies enabled the development of a quantitative RPA microbiome assay capable of determining the relative abundance of the physiologically-important bacterium Akkermansia muciniphila in human feces.
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  • 284_Chen

    Sensitive and rapid detection of pathogenic bacteria from urine samples using multiplex recombinase polymerase amplification.

    Author: Chen J, Xu Y, Yan H, Zhu Y, Wang L, Zhang Y, Lu Y, Xing W
    The chip-based bacteria detection proposed in this study is a promising tool for sensitive, accurate, and multiplex identification of bacteria in clinical urine samples of UTIs and bacteriuria.
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  • 285_Karakkat

    Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification.

    Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.
    Take-all patch, necrotic ring spot, and summer patch are destructive diseases of amenity turfgrasses in temperate climates. LAMP and RPA assays were created for each of the causal agents of the above diseases. Both assays were highly specific to each ...
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  • 286_Nakano

    Fast and sensitive isothermal DNA assay using micro-bead dielectrophoresis for detection of anti-microbial resistance genes.

    Author: Nakano M, Kalsi S, Morgan H.
    Fast and sensitive detection of anti-microbial resistance gene. Combination of isothermal DNA amplification and microbeads-based dielectrophoretic impedance measurement. Amplicon of target gene was attached on microbeads, then, the amplicon-labeled microbeads were detected. Simultaneous amplification and labeling method was proposed. Detection ...
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  • RPA_Cycle

    Editorial for Analytical Biochemistry special issue on RPA

    Author: Tortajada-Genaro LA, O'Sullivan CK, Piepenburg O, Katakis I.
    This Special Issue of Analytical Biochemistry entitled “Recombinase Polymerase Amplification: Applications and Advances” presents an overview of the application of the recombinase-polymerase amplification technology (RPA) in a variety of different contexts. We believe that this Special Issue should be of ...
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