Publications

Every year more and more scientists are finding out that RPA really works. Over 170 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • 1.	Rapid isothermal point mutation detection – towards a first pass screening strategy for multidrug resistant tuberculosis

    Rapid isothermal point mutation detection – towards a first pass screening strategy for multidrug resistant tuberculosis

    Author: Ng BYC, Wee EJH, Woods K, Anderson W, Antaw F, Tsang HZH, West NP, Trau M.
    Herein, we describe a novel strategy that enabled exquisite point mutation discrimination with isothermal DNA amplification, using mismatched primers in conjunction with a two-round enrichment process. As a proof of concept, the method was applied to the rapid and specific ...
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  • Crown Gall Agrobacterium

    Isothermal Amplification and Lateral-Flow Assay for Detecting Crown-Gall-Causing Agrobacterium spp.

    Author: Fuller SL, Savory EA, Weisberg AJ, Buser JZ, Gordon MI, Putnam ML, Chang JH.
    We designed oligonucleotide primers and probes to target virD2 for use in a molecular diagnostic tool that relies on isothermal amplification and lateral-flow-based detection.
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  • Pork meat test

    Determination of porcine ingredient in pork with recombinase polymerase mediated isothermal amplification.

    Author: Guo YH, Chen S, Wang DL, Nie YY, Wu H, Yang J, Zeng GQ, Chen JL, Guo XD.
    This method is rapid and efficient, and has good specificity and sensitivity, which is suitable for the rapid identification of porcine ingredient in meat from the market.
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  • Tuberculosis

    Rapid and visual detection of Mycobacterium tuberculosis complex using recombinase polymerase amplification combined with lateral flow strips

    Author: Ma Q, Liu H, Xiang G, Shan W, Xing W.
    In this study, we introduced a rapid and visual detection method of MTBC using recombinase polymerase amplification (RPA) combined with lateral flow (LF) strips. The LF-RPA assay, read results with naked eyes, could detect as few as 5 genome copies ...
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  • flow-based chemiluminescence microarray

    Quantification of viable and non-viable Legionella spp. by heterogeneous asymmetric recombinase polymerase amplification (haRPA) on a flow-based chemiluminescence microarray

    Author: Kober C, Niessner R, Seidel M.
    In this work, it was shown for the first time that the combination of a propidium monoazide (PMA) treatment with haRPA, the so-called viability haRPA, is able to identify viable Legionella on DNA microarrays.
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  • Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification

    Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification

    Author: Wang J, Wang J, Li R, Liu L, Yuan W.
    The RT-RPA assay was performed successfully at 40 °C, and the results were obtained within 3 min–12 min. The assay could detect CDV, but did not show cross-detection of canine parvovirus-2 (CPV-2), canine coronavirus (CCoV), canine parainfluenza virus (CPIV), pseudorabies virus (PRV) or ...
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  • Long-tailed macaque

    Rapid Detection of Plasmodium knowlesi by Isothermal Recombinase Polymerase Amplification Assay

    Author: Lai MY, Ooi CH, Lau YL.
    The RPA assay exhibited high sensitivity with limits of detection down to one copy of the plasmid. From the specificity experiments, it was demonstrated that all P. knowlesi samples (N = 45) were positive while other Plasmodium spp. (N = 42) and negative samples (N = ...
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  • 1.	Establishment of a Real-time Recombinase Polymerase Amplification Assay for Rapid Detection of African Swine Fever Virus

    Establishment of a Real-time Recombinase Polymerase Amplification Assay for Rapid Detection of African Swine Fever Virus

    Author: Li L, Liu F, Fan X, Li J, Zou Y, Liu S, Bao J, Wu X, Wang Z.
    In this studythe specific primers and exo probes were designed and synthesised by analysing the conserved region of B646L gene of African swine fever virus(ASFV). A real-time fluorescence RPA method for detection of ASFV was established.
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  • Pigs

    Establishment of Real-time Fluorescent Recombinase Polymerase Amplification Technology (RPA) for the detection of Seneca Valley Virus

    Author: Xiaoxu F, Hardenchu ​​C, Wang Y, Wang J, Liu C, Yutian Y, Zhao Y, Zhang Z, Wu X, Wang Z.
    A real-time RPA method was established in this study, based on recombinase polymerase amplification technology (RPA). Assemblies of primers and probes targeting SVV 3D gene were developed and screened, while reaction conditions were optimized before sensitivity, specificity and repeatability of ...
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  • Orf virus is a member of the parapoxvirus genus

    Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus.

    Author: Yang Y, Qin X, Zhang X, Zhao Z, Zhang W, Zhu X, Cong G, Li Y, Zhang Z.
    Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively.
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