Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Salmonella

    Sensitive and rapid visual detection of Salmonella Typhimurium in milk based on recombinase polymerase amplification with lateral flow dipsticks.

    Author: Hu J, Huang R, Sun Y, Wei X, Wang Y, Jiang C, Geng Y, Sun X, Jing J, Gao H, Wang Z, Dong C.
    Salmonella Typhimurium (S. Typhimurium) can cause serious foodborne diseases. In this study, an assay combining recombinase polymerase amplification (RPA) with lateral flow dipsticks (LFD) was developed to detect S. Typhimurium in milk.
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  • geese

    Rapid and sensitive detection of goose parvovirus and duck-origin novel goose parvovirus by recombinase polymerase amplification combined with a vertical flow visualization strip.

    Author: Liu W, Yang Y, Du S, Yi H, Xu D, Cao N, Jiang D, Huang Y, Tian Y.
    Goose parvovirus (GPV) is one of the most serious viral pathogens in goslings. Recently, a new pathogen to the Chinese mainland—duck-origin novel goose parvovirus (N-GPV)—was found to be 90.8–94.6% identical to the nucleotide sequence of GPV, and typically causes growth disorders and high ...
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  • horse

    Development of a rapid and sensitive recombinase polymerase amplification‐lateral flow assay for detection of Burkholderia mallei.

    Author: Saxena A, Pal V, Tripathi NK, Goel AK.
    Early diagnosis of glanders is critical for timely treatment in humans and disease containment in animals. In this study a recombinase polymerase amplification-lateral flow assay has been developed for early and accurate detection of B. mallei.
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  • saffron

    A novel onsite and visual molecular technique to authenticate saffron (Crocus sativus) and its adulterants based on recombinase polymerase amplification.

    Author: Zhao M, Wang B, Xiang L, Xiong C, Shi Y, Wu L, Meng X, Dong G, Xie Y, Sun W.
    Saffron (Crocus sativus), one of the most expensive herbs in China, has been subject to various types of adulteration as a consequence of its high price and limited production. This study describes a sensitive, efficient and simple technique for on-site ...
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  • Mastadenovirus

    Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay.

    Author: Wu T, Wu H, Zhao K, Hu C, Ge Y, Zhu X, Zhang X, Zhou M, Zhu F, Cui L.
    As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, an RPA assay was developed for rapidly detecting HAdVs species B ...
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  • Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids

    A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi.

    Author: Pang J, Wang Q, Fei Y, Zhu P, Qiao L, Huang H, Dang C, Gao W.
    Vibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast ...
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  • Schistosoma_31917985_200px

    Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay.

    Author: Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L, Jia WZ, Fu BQ.
    Accurate diagnosis of Trichinella spp. infection in domestic animals is crucial for the effective prevention and control of human trichinellosis. In the present study, a simple, rapid and accurate diagnostic assay was developed combining recombinase polymerase amplification and a lateral ...
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  • 337_Villamor

    Multilocus characterization, gene expression analysis of putative immunodominant protein coding regions, and development of recombinase polymerase amplification assay for detection of ‘Candidatus Phytoplasma pruni’ in Prunus avium.

    Author: Villamor DEV, Eastwell K.
    Recent escalation of WX disease in Washington State prompted the development of a rapid detection assay based on recombinase-polymerase amplification (RPA) to facilitate timely removal and replacement of diseased trees. This study reports a reliable RPA assay targeting putative immunodominant ...
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  • 336_Zeng

    Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification.

    Author: Zeng R, Luo J, Gao S, Xu L, Song Z, Dai F.
    Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated ...
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  • Recombinase polymerase amplification project for Ebola detection

    Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka.

    Author: Gunaratna G, Manamperi A, Bohlken-Fascher S, Wickremasinge R, Gunawardena K, Yapa B, Pathirana N, Pathirana H, de Silva N, Sooriyaarachchi M, Deerasinghe T, Mondal D, Ranasinghe S, Abd El Wahed A.
    In this study, a mobile suitcase laboratory applying novel extraction (SpeedXtract) and isothermal amplification and detection (recombinase polymerase amplification assay, RPA) methods were evaluated for the diagnosis of cutaneous leishmaniasis in Sri Lanka.
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