Publications

Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Coxiella burnetii Q Fever

    Rapid and visual detection of Coxiella burnetii using recombinase polymerase amplification combined with lateral flow strips

    Author: Qi Y, Yin Q, Shao Y, Li S, Chen H, Shen W, Rao J, Li J, Li X, Sun Y, Lin Y, Deng Y, Zeng W, Zheng S, Liu S, Li Y.
    The detection system has a good specificity for detection of C. burnetii xinqiao 18 strain without cross-reaction with R.rickettsii, R.heilongjiangensis, R.sibirica, O.19 tsutsugamushi, S.aureus, orS.suis. It can detect recombinant 23SrRNA-pUC1920 plasmid and genomic DNA at levels of 10 copies/reaction and ...
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  • First report of Pythium root rot caused by Pythium mastophorum on parsley in the United States

    First report of Pythium root rot caused by Pythium mastophorum on parsley in the United States

    Author: Tsuchida C, Mauzey S, Hatlen R, Miles TD, Koike ST.
    We believe this is the first report of P. mastophorum infecting parsley in California. P. mastophorum has been reported on parsley only in southeastern Australia (Petkowski et al., 2013). In California, this species has been reported on celery (Vazquez et ...
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  • A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitides

    A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitides

    Author: Rivas L, Reutersward P, Rasti R, Herrmann B, Martensson A, Alfven T, Gantelius J, H Andersson-Svahn.
    In this article, we describe a colorimetric vertical-flow DNA microarray (DNA-VFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA).
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  • Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

    Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

    Author: Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F.
    Through characterization of CRISPR enzymology and application development, we report here four advances integrated into SHERLOCKv2: 1) 4-channel single reaction multiplexing using orthogonal CRISPR enzymes; 2) quantitative measurement of input down to 2 aM; 3) 3.5-fold increase in signal sensitivity ...
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  • CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

    CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

    Author: Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA.
    By combining Cas12a ssDNase activation with isothermal amplification, we create a method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR), which achieves attomolar sensitivity for DNA detection.
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  • Rapid Detection of Vibrio parahaemolyticus in Shellfish by Real-Time Recombinase Polymerase Amplification

    Rapid Detection of Vibrio parahaemolyticus in Shellfish by Real-Time Recombinase Polymerase Amplification

    Author: Zhu P, Gao W, Huang H, Jiang J, Chen X, Fan J, Yan X.
    The detection limit of this assay was 0.4 pg/μL of DNA, which is comparable to assays that use the bacterial culture as template, 4 × 103 cfu/mL.
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  • Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis

    Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis.

    Author: Shahin K, Ramirez-Paredes JG, Harold G, Lopez-Jimena B, Adams A, Weidmann M.
    The RPA gave quicker results in approximately 6 min in contrast to the qPCR that needed about 90 min to reach the same detection limit, taking only 2.7-3 min to determine Fno in clinical samples. Moreover, RPA was more tolerant ...
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  • RPA using a multiplexed cartridge for low cost point of care diagnostics in the field

    RPA using a multiplexed cartridge for low cost point of care diagnostics in the field

    Author: Ereku LT, Mackay RE, Craw P, Naveenathayalan A, Stead T, Branavan M, Balachandran W.
    This paper shows the results from two separate experiments: the first using the RPA control nucleic acid, the second showing successful amplification from Chlamydia Trachomatis.
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  • Heterogeneous asymmetric recombinase polymerase amplification for rapid hygiene control of large-volume water samples

    Heterogeneous asymmetric recombinase polymerase amplification (haRPA) for rapid hygiene control of large-volume water samples

    Author: Elsasser D, Ho J, Niessner R, Tiehm A, Seidel M.
    Field measurements were conducted to test the developed system for hygiene online monitoring under realistic conditions. We could show that this system allows the detection of artificial contaminations of bacteriophage PhiX174 in drinking water pipelines.
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  • Real-time quantitative isothermal detection of ostreid herpesvirus-1 DNA in Scapharca subcrenata using recombinase polymerase amplification

    Real-time quantitative isothermal detection of ostreid herpesvirus-1 DNA in Scapharca subcrenata using recombinase polymerase amplification

    Author: Gao F, Jiang J-Z, Wang J-Y, Wei H-Y.
    The detection limit for qRPA assays was shown to be 5 copies DNA/reaction for the primer set ORF95, which was lower than the 100 copies required for the qPCR test. The optimal reaction temperature and time were 37 °C for ...
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