Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • 340_Dey

    An integrated multi-molecular sensor for simultaneous BRAFV600E protein and DNA single point mutation detection in circulating tumour cells.

    Author: Dey S, Koo K, Wang Z, Sina AAI, Wuethrich A, Trau M.
    This study presents an integrated multi-molecular sensor (IMMS) for an entire sample-to-answer workflow from melanoma cell capture to simultaneous quantification of both intracellular BRAFV600E DNA and protein levels on a single platform.
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  • Cows1_small

    Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis.

    Author: Zhao G, Hou P, Huan Y, He C, Wang H, He H.
    Mycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. ...
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  • Rapid salmonella detection using RT-RPA

    Real-time recombinase polymerase amplification assay for the rapid and sensitive detection of Campylobacter jejuni in food samples.

    Author: Geng Y, Liu G, Lui L, Deng Q, Zhao L, Sun XX, Wang J, Zhao B, Wang J.
    In this study, a real-time RPA assay was developed so as to achieve the rapid and efficient detection of C. jejuni by targeting the hipO gene. The specificity and sensitivity of real-time RPA was validated and the practical applicability of ...
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  • 299_Ma

    Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus.

    Author: Ma L, Zeng F, Cong F, Huang B, Zhu Y, Wu M, Xu F, Yuan W, Huang R, Guo P.
    Murine norovirus (MNV) is recognized as the most prevalent viral pathogen in captive mouse colonies. The rapid detection assay for MNV would be a useful tool for monitoring and preventing MNV infection. A recombinase polymerase amplification (RPA) assay was established ...
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  • Leaves_271571255

    Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of "Candidatus Liberibacter asiaticus".

    Author: Ghosh DK, Kokane SB, Kokane AD, Warghane AJ, Motghare MR, Bhose S, Sharma AK, Reddy MK.
    Currently, polymerase chain reaction (PCR) and real time PCR have been the gold standard techniques used for detection of ‘Ca. L. asiaticus’. These diagnostic methods are expensive, require well equipped laboratories, not user-friendly and not suitable for on-site detection of ...
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  • Goat 2_282513164

    Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.

    Author: Li Y, Li L, Fan X, Zou Y, Zhang Y, Wang Q, Sun C, Pan S, Wu X, Wang Z.
    Current detection of PPRV in clinical samples mainly relies on real-time RT-PCR. Particularly, samples collected from rural area require highly equipped laboratories for screening. A rapid, real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA), employing primers and exo probe, was thus ...
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  • 339_Kim

    Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification.

    Author: Kim NY, Oh J, Lee SH, Kim H, Moon JS, Jeong RD.
    In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA ...
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  • Salmonella

    Capture, Concentration and Detection of Salmonella in Foods Using Magnetic Ionic Liquids and Recombinase Polymerase Amplification.

    Author: Hice SA, Clark KD, Anderson JL, Brehm-Stecher BF.
    This study combines magnetic ionic liquid (MIL)-based sample preparation with isothermal amplification and detection of Salmonella-specific DNA using Recombinase Polymerase Amplification (RPA).
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  • 328_Nybond

    Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.

    Author: Nybond S, Reu P, Rhedin S, Svedberg G, Alfven T, Gantelius J, Svahn HA.
    Described in this study is the use of isothermal amplification (RPA) of viral DNA at 37 °C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles.
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  • Rickettsia bacteria

    Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test.

    Author: Qi Y, Shao Y, Rao J, Shen W, Yin Q, Li X, Chen H, Li J, Zeng W, Zheng S, Liu S, Li Y.
    Rickettsia rickettsii is the causative agent of Rocky Mountain spotted fever, which is the most severe spotted fever group (SFG) rickettsiosis. In this study a rapid, visual, sensitive and specific method for the detection of R. rickettsii based on RPA ...
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