Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • 326_Koo

    A Sample-to-Targeted Gene Analysis Biochip for Nanofluidic Manipulation of Solid-Phase Circulating Tumor Nucleic Acid Amplification in Liquid Biopsies.

    Author: Koo KM, Dey S, Trau M.
    In this study, complex liquid biopsy sample-to-targeted genetic analysis was conducted on a biochip with a 50 copies-detection limit within 30 min. The biochip uniquely integrated electrical lysis and release of cellular targets with minimal processing; nanofluidic manipulation to accelerate ...
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  • African Cows

    Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1.

    Author: Wang H, Hou P, Zhao G, Yu L, Gao Y, He H.
    Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV) is one of the most highly infectious diseases in livestock, and leads to huge economic losses. In this study, a series of serotype-specific reverse transcription recombinase polymerase amplification assays combined with ...
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  • Rapid detection of salmonella using real-time RPA

    Development of a reverse transcription recombinase polymerase amplification combined with lateral-flow dipstick assay for avian influenza H9N2 HA gene detection.

    Author: Wang Z, Yang P, Zhang Y, Tian K, Bian C, Zhao J.
    Simple, rapid, sensitive and accurate methods suitable for field detection of H9N2 AIVs are crucial to efficiently control virus infection and spread in time. In this study, an isothermal reverse transcription recombinase polymerase amplification with lateral‐flow dipstick (RT‐RPA‐LFD) assay for ...
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  • 323_Zhao

    Reverse transcription-recombinase polymerase amplification combined with lateral flow strip for detection of rice black-streaked dwarf virus in plants

    Author: Zhao C, Sun F, Li X, Lan Y, Du L, Zhou T, Zhou Y.
    Rice black-streaked dwarf virus (RBSDV) infects rice plants, a major crop, and is transmitted via the small brown planthopper (SBPH: Laodelphax striatellus Fallén), causing significant economic loss in China. To rapidly diagnose RBSDV, a reverse transcription-recombinase polymerase amplification (RT-RPA) method ...
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  • Tomatoes_3881845

    Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.

    Author: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M.
    Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. ...
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  • 321_Sun

    Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.

    Author: Sun N, Wang W, Wang J, Yao X, Chen F, Li X, Yinglei Y, Chen B.
    Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. The sensitivity of RT-RPA-LFD assays is ...
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  • Cows1_small

    Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk.

    Author: Hou P, Zhao G, Wang H, He C, He H.
    The aim of this study was to develop a novel isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), for rapid detection of BVDV. The detection limit of this assay was 20 copies per reaction, and ...
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  • Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis

    Rapid detection of Listonella anguillarum by recombinase polymerase amplification technique.

    Author: JianHu P, LongLiang Q, HaiLong H, ChenYang D, Peng Z, JiLin X.
    In this study, several pairs of primers and an exo-probe targeting the zinc metalloprotease gene (empA) of Listonella anguillarum was designed and a rapid real-time detection method for Listonella anguillarum based on recombinase polymerase amplification (RPA) was established by optimizing ...
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  • Application of recombinase polymerase amplification in the detection of Pseudomonas aeruginosa

    Rapid detection of mcr-1 by recombinase polymerase amplification

    Author: Xu J, Wang X, Yang L, Kan B, Lu X.
    RPA detection based on the mcr-1 gene was successfully applied in our study. The plasmid-mediated mcr-1 gene conferring colistin drug resistance has a strong ability to spread, suggesting the need to further strengthen the detection of this resistance gene in ...
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  • 317_Li

    Rapid detection of cyanobacteria by recombinase polymerase amplification combined with lateral flow strips.

    Author: Li J, Wang C, Yu X, Lin H, Hui C, Shuai L, Zhang S.
    Cyanobacteria are one of the major groups of algae causing algal blooms. In this study a rapid method for detecting Cyanobacteria was developed using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. The method developed in ...
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