Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Development of a Reverse Transcription-Recombinase Polymerase Amplification Assay for Detection of Sugarcane Yellow Leaf Virus

    Reverse Transcription–Recombinase Polymerase Amplification Assay for the Detection of Sugarcane Streak Mosaic Virus in Sugarcane.

    Author: Feng XY, Shen LB, Wang WZ, Wang JG, Cao ZY, Zhang SZ.
    In this study, a novel isothermal assay was established on the basis of reverse transcription–recombinase polymerase amplification (RT-RPA) and evaluated in terms of its ability to detect SCSMV. Overall, the RT-RPA assay was specific, sensitive, reliable, and rapid for SCSMV ...
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  • 277_Burkhardt2

    Detection of Fusarium oxysporum f. sp. fragariae from infected strawberry plants.

    Author: Burkhardt A, Henry PM, Koike ST,Gordon T, Martin FN.
    In this study, next-generation sequencing and comparative genomics were used to identify a unique genetic locus that can detect all the somatic compatibility groups of F. oxysporum f. sp. fragariae identified in California. This locus was used to develop a ...
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  • 316_Subbotin

    Recombinase polymerase amplification assay for rapid detection of the root-knot nematode Meloidogyne enterolobii.

    Author: Subbotin SA.
    RPA assays were developed targeting the IGS rRNA gene of the pacara earpod tree root-knot nematode, Meloidogyne enterolobii. The RPA assays using TwistAmp® Basic and TwistAmp® exo kits allowed detection of M. enterolobii from gall tissues and crude nematode extracts ...
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  • cat

    Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1.

    Author: Liu MZ, Han XH, Yao LQ, Zhang WK, Liu BS, Chen ZL.
    A recombinase polymerase amplification (RPA) assay (RPA-LFD-FHV) combined with a lateral flow dipstrip (LFD) was developed that uses human body heat for incubation. The RPA-FLD-FHV assay is a simple, rapid, and reliable method for point-of-care diagnosis of FHV-1 infection.
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  • Yersenia Pestis Plague

    Duplex lateral flow assay for the simultaneous detection of Yersinia pestis and Francisella tularensis.

    Author: Jauset-Rubio M, Tomaso H, Soror El-Shahawi M, Saleh Omar Bashammakh A, Obaid Al-Youbi A, O' Sullivan CK.
    The multiplexed amplification detection of genomic DNA from Francisella tularensis and Yersinia pestis is reported here. Amplification was achieved using isothermal recombinase polymerase amplification (RPA), exploiting tailed primers, followed by detection using a nucleic-acid lateral flow assay.
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  • Canine parovirus

    Detection of Babesia gibsoni in dogs by combining recombinase polymerase amplification (RPA) with lateral flow (LF) dipstick.

    Author: Cui J, Zhao Y, Sun Y, Yu L, Liu Q, Zhan X, Li M, He L, Zhao J.
    The novel LF-RPA assay is effective for the detection of B. gobsini and has considerable advantages over the conventional PCR in sensitivity, specificity, simplicity in operation, less time consumption, and visual detection. The LF-RPA method may facilitate the surveillance and ...
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  • Sanchez_Salcedo

    On‐gold recombinase polymerase primer elongation for the electrochemical detection of bacterial genome: mechanism insights and influencing factors.

    Author: Sánchez-Salcedo R, Miranda-Castro R, de-los-Santos-Álvarez N, Jesus Lobo Castañón M.
    Here, an on‐chip isothermal recombinase polymerase amplification (RPA) with chronoamperometric detection, which could be run with simple equipment was optimised. A short thiol‐modified oligonucleotide, complementary to a fragment of bipA gene of Salmonella spp., is chemisorbed onto the gold chip ...
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  • Wang

    “Mix-to-Go” Silver Colloidal Strategy for Prostate Cancer Molecular Profiling and Risk Prediction.

    Author: Wang J, Koo KM, Wang Y, Trau M.
    Herein, is proposed a “mix-to-go” colloidal strategy which utilizes the electrostatic attraction between negatively charged target sequences and positively charged silver nanoparticles (AgNPs) to induce aggregation of AgNPs, to profile a panel of clinically-validated urinary prostate cancer (PCa) RNA biomarkers ...
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  • Rapid salmonella detection using RT-RPA

    Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification.

    Author: Ma S, Li X, Peng B, Wu W, Wang X, Liu H, Yuan L, Fang S, Lu J.
    In this study, a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9 was developed and evaluated. The H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic ...
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  • Norovirus_121829743

    Low-Cost Detection of Norovirus Using Paper-Based Cell-Free Systems and Synbody-Based Viral Enrichment.

    Author: Ma D, Shen L, Wu K, Diehnelt CW, Green AA.
    Herein is demonstrated a low-cost colorimetric assay that detects norovirus from clinical samples by combining paper-based cell-free transcription-translation systems, isothermal amplification, and virus enrichment by synbodies. Using isothermal amplification and cell-free RNA sensing with toehold switches, it was demonstrated that ...
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