Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Tomatoes_3881845

    Recombinase Polymerase Amplification Assay for Field Detection of Tomato Bacterial Spot Pathogens.

    Author: Strayer-Scherer AL, Jones LB, Paret ML.
    Recombinase polymerase amplification (RPA) is ideal for in-field detection assays because it is an isothermal technique that is rapid and more tolerant to inhibitors compared to PCR. Hence, novel RPA probes and primers were designed to amplify regions of the ...
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  • E coli

    High-specificity Double-stranded DNA Detection with “Humanoid” Molecular Beacon and TALEs.

    Author: Wang Y, Bai LJ, Qu X, Gao Y, Wang J, Li S, Fan L, Wei H, Liu S, Peng Y, Gao Z, Zhu Y, Gao Z, Ning B.
    Owing to the limitation of DNA-specific recognition, improving the sensitivity of bacteria detection without additional culture poses a considerable challenge. Herein, a sensitive, novel, and high-specificity fluorescence detection method was developed using transcription activator-like effectors (TALEs) as the specifically recognized ...
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  • Salmonella

    Recombinase Polymerase Amplification Combined with Unmodified Gold Nanoparticles for Salmonella Detection in Milk.

    Author: Chen ZG, Zhong HX, Luo H, Zhang RY, Huang JR.
    In this study, isothermal recombinase polymerase amplification (RPA) was combined with unmodified gold nanoparticles (AuNPs) to detect Salmonella in milk. A rapid, effective, visualized, and low-cost RPA-AuNP assay was developed, with a detection limit of 50 CFU for milk samples ...
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  • Yersenia Pestis Plague

    Isothermal DNA amplification combined with lateral flow dipsticks for detection of biothreat agents.

    Author: Zasada AA, Zacharczuk K, Forminska K, Wiatrzyk A, Ziolkowski R, Malinowska E.
    In the presented study three methods of isothermal DNA amplification loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA) and thermophilic helicase-dependent isothermal DNA amplification (tHDA), were compared for detection of highly dangerous pathogens, such as Bacillus anthracis, Francisella tularensis and ...
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  • Screen Shot 2017-02-22 at 3.42.06 pm

    Visual and equipment-free reverse transcription recombinase polymerase amplification method for rapid detection of foot-and-mouth disease virus.

    Author: Liu L, Wang J, ZhangR, Lin M, Shi R, Han Q, Wang J, Yuan W.
    A visible and equipment-free reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip (LFS RT-RPA) was developed to detect the FMDV using primers and LF probe specific for the 3D gene.
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  • 300_Yang

    A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids.

    Author: Yang H, Chen Z, Cao X, Li Z, Stavrakis S, Choo J, deMello AJ, Howes PD, He N.
    An integrated sample-in-digital-answer-out (SIDAO) diagnostic system incorporating DNA extraction and digital recombinase polymerase amplification, which enables rapid and quantitative nucleic acid analysis from bodily fluids within a disposable cartridge.
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  • Streptococcus_493890658

    Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.

    Author: Chen J, Wang Y, Liu X, Chen G, Chen X, Chen J, Liu Z, Gong J, Yang G, Lan Q.
    To determine whether propidium monoazide (PMA) can eliminate the influence of DNA extracted from dead cells, a bacterial suspension was treated with PMA before DNA extraction. Findings of RPA assay showed that DNA extracted from dead cells had no fluorescence ...
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  • 299_Ma

    Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus.

    Author: Ma L, Cong F, Zhu Y, Wu M, Xu F, Huang R, Moore RJ, Guo P.
    A reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. The sensitivity of the RT-RPA assay approached 8 copies per reaction, which is equivalent to the sensitivity of RT-qPCR reactions.
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  • 297_Singpanomchai2

    Naked eye detection of the Mycobacterium tuberculosis complex by recombinase polymerase amplification-SYBR green I assays.

    Author: Singpanomchai N, Akeda Y, Tomono K, Tamaru A, Santanirand P, Ratthawongjirakul P.
    Rapid diagnosis of Mycobacterium tuberculosis (Mtb) is key to controlling the spread of tuberculosis, which is a global health concern. In this study, isothermal recombinase polymerase amplification (RPA) was developed to detect specific targets of Mtb, IS6110 and IS1081. Additionally, ...
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  • Cows1_small

    A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.

    Author: Zhao G, Wang H, Hou P, Xia X, He H.
    In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA).
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