Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Napier grass

    Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses.

    Author: Karakkat BB, Hockemeyer K, Franchett M, Olson M, Mullenberg C, Koch PL.
    The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples.
    Read more
  • 294_Koo

    Arch-shaped multiple-target sensing for rapid diagnosis and identification of emerging infectious pathogens.

    Author: Koo B, Hong KH, Jin CE, Kim JY, Kin SH, Shin Y.
    In this study, an arch-shaped amplification containing primer sequences was designed to rapidly amplify multiple targets. Further, the sensing platform allowed for sensitive and specific detection of human coronavirus, Middle East respiratory syndrome, Zika virus, and Ebola virus down to ...
    Read more
  • 295_Higgins

    PrimedRPA: Primer design for Recombinase polymerase amplification assays.

    Author: Higgins M, Ravenhall M, Ward D, Phelan J, Ibrahim A, Forrest MS, Clark TG, Campino S.
    PrimedRPA is a python-based package that automates the creation and filtering of RPA primers and probe sets. It aligns several sequences to identify conserved targets, and filters regions that cross react with possible background organisms.
    Read more
  • 292_Ahn

    Single-Step Recombinase Polymerase Amplification Assay Based on a Paper Chip for Simultaneous Detection of Multiple Foodborne Pathogens.

    Author: Ahn H, Batule BS, Seok Y, Kim MG.
    A paper chip device-based recombinase polymerase amplification (RPA) method was developed for highly sensitive and selective single-step detection of foodborne pathogens. The diagnostic utility of the device was demonstrated by the reliable detection of E. coli and S.aureus present in ...
    Read more
  • 293_Huang

    BioBits™ Explorer: A modular synthetic biology education kit.

    Author: Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ.
    BioBits™ Explorer kit enables hands-on demonstrations of cutting-edge science that are inexpensive and easy to use, circumventing many current barriers for implementing exploratory biology experiments in classrooms.
    Read more
  • 290_Toldra

    Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay.

    Author: Toldra A, Jauset-Rubio M, Andree KB, Fernandez-Tejedor M, Diogene J, Katakis I, O'Sullivan CK, Campas M.
    An assay for the detection of two Karlodinium species has been developed. The assay is based on isothermal DNA amplification and a sandwich hybridisation. The assay discriminates between Karlodinium veneficum and Karlodinium armiger. Seawater samples spiked with Karlodinium cells have ...
    Read more
  • 291_Wang

    Rapid detection of foot-and-mouth disease virus using reverse transcription recombinase polymerase amplification combined with a lateral flow dipstick.

    Author: Wang H, Zhao G, Hou P, Yu L, He C, He H.
    The RPA-LFD assay was highly sensitive, detecting down to 10 copies of plasmid DNA. There was no cross-reactivity with other pathogens causing vesicular lesions. In addition, 143 clinical samples were used to compare RPA-LFD with real-time PCR, with 98.6% concordance ...
    Read more
  • 289_Hu2

    Rapid and visual detection of Group B streptococcus using recombinase polymerase amplification combined with lateral flow strips.

    Author: Hu S, Zhong H, Huang W, Zhan W, Yang X, Tang B, Chen K, Wang J, Hu T, Zhang C, Zhou Z, Luo M.
    A rapid molecular method for GBS detection was developed and evaluated. This assay has high specificity and sensitivity for diagnosing GBS, which is suitable for clinical application. The assay can be completed within about 30 minutes without the need of ...
    Read more
  • 288_Koo

    Design and Clinical Verification of Surface Enhanced Raman Spectroscopy Diagnostic Technology for Individual Cancer Risk Prediction.

    Author: Koo KM, Wang J, Richards RS, Farrell A, Yaxley JW, Samaratunga H, Teloken PE, Roberts MJ, Coughlin GD, Lavin MF, Mainwaring PN, Wang Y, Gardiner RA, Trau M.
    Describes a comprehensive clinical verification of a prospective label-free surface-enhanced Raman scattering (SERS) nanodiagnostic assay for prostate cancer (PCa) risk stratification.
    Read more
  • Canine parovirus

    Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus.

    Author: Wang J, Wang J, Li R, Shi R, Liu L, Yuan W.
    Visual, rapid molecular assay for detection of CDV was developed. Assay is based on RT-RPA and use of a lateral flow strip to visualize product. Assay was performed in closed fists using body heat for 15 min. Analytical sensitivity and specificity ...
    Read more