Publications

Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Ebola 2

    Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future

    Author: James AS, Todd S, Pollak NM, Marsh GA, Macdonald J.
    The assay had a detection limit of 134 copies per μL of plasmid containing the NP gene of Ebolavirus Mayinga, and cultured Ebolavirus and was highly specific for the Zaire ebolavirus species, including the Guinea strain responsible for the 2014/2015 ...
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  • Application of recombinase polymerase amplification in the detection of Pseudomonas aeruginosa

    Application of recombinase polymerase amplification in the detection of Pseudomonas aeruginosa

    Author: Jin XJ, Gong YL, Yang L, Mo BH, Peng YZ, He P, Zhao JN, Li XL.
    The detection limit of Pseudomonas aeruginosa in real-time PCR and PCR was 1×10 7 CFU/mL, and the detection limit of Pseudomonas aeruginosa in RPA was 1×10 2 CFU/mL.RPA. In fluorescence quantitative PCR, the higher the concentration of Pseudomonas aeruginosa, the ...
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  • A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia

    A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia

    Author: Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M.
    The analytical sensitivity in the multiplex assay amplifying specific regions of S. pneumoniae and L. pneumophila simultaneously was 10 CFUs of genomic DNA per reaction. In cross detection studies with closely related strains and other bacterial agents the specificity of the ...
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  • Isothermal amplification and lateral flow detection of Plasmodium

    Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus

    Author: Tan KK, Azizan NS, Yaacob CN, Che Mat Seri NAA, Samsudin NI, Teoh BT, Sam SS, AbuBakar S.
    The dengue RT-RPA assay can be successfully performed by simply following the provided written instructions. Deviations from the written protocols did not adversely affect the outcome of the assay. These suggest that the RT-RPA assay is indeed a simple, robust ...
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  • Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids

    Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids

    Author: Soliman H, Kumar G, El-Matbouli M.
    A novel PKD-RPA assay for the detection of T. bryosalmonae was developed. The assay offers considerable advantages including speed, sensitivity, specificity and visual detection. Applying the PKD-RPA assay combined with an LFD enhances the surveillance and early detection of T. ...
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  • 258_Jin

    Simple and label-free pathogen enrichment via homobifunctional imidoesters using a microfluidic (SLIM) system for ultrasensitive pathogen detection in various clinical specimens

    Author: Jin CE, Koo B, Lee EY, Kim JY, Kim SH, Shin Y.
    We demonstrated its clinical utility in large sample volumes from 46 clinical specimens including environmental swabs, saliva, and blood plasma. The SLIM system showed higher sensitivity with these samples and could detect pathogens that were below the threshold of detection ...
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  • Bacillus anthracis (Anthrax)

    Sensitive and specific recombinase polymerase amplification set of assays for fast screening, detection and identification of Bacillus anthracis in a field setting.

    Author: Bentahir M, Ambroise J, Delcorps C, Pilo P, Gala JL.
    RPA tests displayed 100% specificity and sensitivity. The hands-off turnaround time at 42°C ranged from 5 to 6 min for 102 genomic copies. The analytical sensitivity of each RPA was ∼10 molecules per reaction. Besides, BA_5345 and adk RPA assays ...
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  • Tick on skin_183149369

    Simultaneous detection of Theileria annulata and Theileria orientalis infections using recombinase polymerase amplification

    Author: Hassan MA, Liu J, Sajid MS, Rashid M, Mahmood A, Abbas Q, Guan G, Yin H, Luo J.
    The multiplex RPA specifically amplified 282-bp and 229-bp fragments of the enolase gene from T. annulata and T. orientalis and had no cross-reaction with other piroplasm species. It was determined that 45 (23.9%) and 5 (2.6%) out of 188 blood ...
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  • detection of isothermally amplified HOTAIR long non-coding RNA

    Naked-eye and electrochemical detection of isothermally amplified HOTAIR long non-coding RNA

    Author: Islam MN, Moriam S, Umer M, Phan HP, Salomon C, Kline R, Nguyen NT, Shiddiky MJA.
    Herein, we report on the development of a new colorimetric and electrochemical assay platform for long non-coding HOX transcript antisense intergenic RNA (HOTAIR) detection. Isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) was performed to amplify HOTAIR sequences from a RNA pool ...
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  • Washing-Free Electrochemical Detection of Amplified Double-Stranded DNAs Using a Zinc Finger Protein

    Washing-Free Electrochemical Detection of Amplified Double-Stranded DNAs Using a Zinc Finger Protein.

    Author: Fang CS, Kim KS, Ha DT, Kim MS, Yang H.
    The whole detection is performed within 17 min (15 min for the RPA reaction and <2 >
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