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Read latest blog post...
Bringing you the latest ideas, opinions and news from the RPA community.
Detection: a multitude of options
Many RPA users will want to be able to detect that DNA amplification has occurred and monitor this over the course of a reaction. When we first developed the technology, we needed to create RPA-specific methods for achieving this. Resolving the product of your reaction on an agarose or polyacrylamide gel is the most common method for post-PCR detection, yet this is out of the question for high throughput activities or point-of-care applications, and undermines our aim of simplifying the equipment and protocols of molecular diagnostic techniques...
Lyophilised or liquid?
One of our key aims at TwistDx is to make the RPA workflow as simple as possible, eliminating the pipetting of multiple reagents and freeing the user to focus on their research. As a result, we now offer RPA kits in two formats – lyophilised pellets and a liquid – each with unique strengths, suitable for different applications and requirements...
TwistDx: A history of science, serendipity and success
Not everyone knows that TwistDx was set up to explore an idea for single molecule sequencing, rather than DNA amplification and detection. One of the tasks was finding a mechanism to create defined ends in small fragments of target DNA, which could then serve as sites for the assembly of the enzymatic sequencing complex...
Who are the innovators pioneering the future of RPA?
We had high hopes when we started developing RPA in 2002, but we could not have anticipated the widespread and innovative applications we see today. RPA is fast establishing itself as being ahead of the field, thanks to the hard work and pioneering spirits of our customers, and we are excited to see the future applications yet to be discovered...
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