Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay
This study successfully utilizes RPA for the detection of M. ulcerans in Buruli ulcer disease
Publications
PublicationsPost Type
An integrated multi-molecular sensor for simultaneous BRAFV600E protein and DNA single point mutation detection in circulating tumour cells.
This study presents an integrated multi-molecular sensor (IMMS) for an entire sample-to-answer workflow from melanoma cell capture to simultaneous quantification of both intracellular BRAFV600E DNA and protein levels on a single platform.
Real-time recombinase polymerase amplification assay for the rapid and sensitive detection of Campylobacter jejuni in food samples.
In this study, a real-time RPA assay was developed so as to achieve the rapid and efficient detection of C. jejuni by targeting the hipO gene. The specificity and sensitivity of real-time RPA was validated and the practical applicability of the method for the detection of C. jejuni in artificially contaminated milk and chicken breast samples was proved by comparing
Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.
Current detection of PPRV in clinical samples mainly relies on real-time RT-PCR. Particularly, samples collected from rural area require highly equipped laboratories for screening. A rapid, real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA), employing primers and exo probe, was thus developed.
Reverse Transcription–Recombinase Polymerase Amplification Assay for the Detection of Sugarcane Streak Mosaic Virus in Sugarcane.
In this study, a novel isothermal assay was established on the basis of reverse transcription–recombinase polymerase amplification (RT-RPA) and evaluated in terms of its ability to detect SCSMV. Overall, the RT-RPA assay was specific, sensitive, reliable, and rapid for SCSMV detection, with a flexible and easily reachable reaction temperature. Thus, it can be an ideal method for SCSMV detection in
Duplex lateral flow assay for the simultaneous detection of Yersinia pestis and Francisella tularensis.
The multiplexed amplification detection of genomic DNA from Francisella tularensis and Yersinia pestis is reported here. Amplification was achieved using isothermal recombinase polymerase amplification (RPA), exploiting tailed primers, followed by detection using a nucleic-acid lateral flow assay.
Detection of Fusarium oxysporum f. sp. fragariae from infected strawberry plants.
In this study, next-generation sequencing and comparative genomics were used to identify a unique genetic locus that can detect all the somatic compatibility groups of F. oxysporum f. sp. fragariae identified in California. This locus was used to develop a TaqMan qPCR assay and an isothermal recombinase polymerase amplification (RPA) assay that have very high sensitivity and specificity for over
Detection of Fusarium oxysporum f. sp. fragariae from infected strawberry plants. Read More
Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk.
The aim of this study was to develop a novel isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), for rapid detection of BVDV. The detection limit of this assay was 20 copies per reaction, and there was no cross-reactivity with other bovine infectious viruses.
Rapid detection of cyanobacteria by recombinase polymerase amplification combined with lateral flow strips.
Cyanobacteria are one of the major groups of algae causing algal blooms. In this study a rapid method for detecting Cyanobacteria was developed using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. The method developed in this study is simple, rapid, and effective for on-site testing of Cyanobacteria, which may become a routine measurement in efforts
Detection of Babesia gibsoni in dogs by combining recombinase polymerase amplification (RPA) with lateral flow (LF) dipstick.
The novel LF-RPA assay is effective for the detection of B. gobsini and has considerable advantages over the conventional PCR in sensitivity, specificity, simplicity in operation, less time consumption, and visual detection. The LF-RPA method may facilitate the surveillance and early detection of B. gibsoni infection in dogs.