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Detection of Entamoeba histolytica by RPA

Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic …

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Application of Isothermal Amplification Techniques for the Identification of Madurella mycetomatis

Both isothermal amplification techniques show high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques is assessed in comparison to conventional PCR using biopsies from eumycetoma patients. RPA is reliable and easy to operate and has a potential to be implemented in mycetoma endemic areas.

Bridging flocculation assay for rapid detection of gene specific DNA methylation

The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples.

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