Publications

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Paper & plastic device for combined isothermal amplification & lateral flow detection of Plasmodium

Novel RPA primers were developed that bind to sequences present in the four species of Plasmodium which infect humans. The paper and plastic devices were found to be capable of detecting as few as 5 copies/µL of synthetic Plasmodium DNA (50 copies total), comparable to the same reaction run on the bench top. The devices produce visual results in an […]

Paper & plastic device for combined isothermal amplification & lateral flow detection of Plasmodium Read More

Bridging flocculation assay for rapid detection of gene specific DNA methylation

The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples.

Bridging flocculation assay for rapid detection of gene specific DNA methylation Read More

Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Gold Nanoparticles

The assay is capable of detecting a positive differential pulse voltammetry (DPV) response from as low as 1 CFU of Mtb bacilli DNA input material, having shown its exquisite sensitivity over a conventional gel based readout. The translation of our assay onto a portable potentiostat was also demonstrated, with promising results. We believe that our assay has significant potential for translation into

Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Gold Nanoparticles Read More

Colorimetric detection of total genomic and loci-specific DNA methylation from limited DNA inputs

Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine diagnostics and research.

Colorimetric detection of total genomic and loci-specific DNA methylation from limited DNA inputs Read More

Application of Isothermal Amplification Techniques for the Identification of Madurella mycetomatis

Both isothermal amplification techniques show high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques is assessed in comparison to conventional PCR using biopsies from eumycetoma patients. RPA is reliable and easy to operate and has a potential to be implemented in mycetoma endemic areas.

Application of Isothermal Amplification Techniques for the Identification of Madurella mycetomatis Read More

Detection of Entamoeba histolytica by RPA

Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic

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Development of RPA Assays for Detection of Orientia tsutsugamushi or Rickettsia typhi

Sensitive, specific and rapid diagnostic tests for the detection of Orientia tsutsugamushi (O.tsutsugamushi)and Rickettsia typhi (R. typhi), the causative agents of scrub typhus and murine typhus, respectively, are necessary to accurately and promptly diagnose patients and ensure that they receive proper treatment. Recombinase polymerase amplification (RPA) assays using a lateral flow test (RPA-nfo) and real-time fluorescent detection (RPA-exo) were developed targeting the 47-kDa gene of O. tsutsugamushi or

Development of RPA Assays for Detection of Orientia tsutsugamushi or Rickettsia typhi Read More

A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care

We developed a diagnostic test that uses isothermal recombinase polymerase amplification (RPA) to detect Leishmania infantum. This method was coupled with lateral flow (LF) reading with the naked eye to be adapted as a point-of-care test. The L. infantum RPA-LF had an analytical sensitivity similar to real time-PCR, detecting DNA of 0.1 parasites spiked in dog blood, which was equivalent to 40 parasites/mL.

A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care Read More

Development of portable and rapid assay for detection of emerging avian influenza A (H7N9) virus

Highlights – The mobile suitcase laboratory is easy to use in low-resource settings. The Diagnostics-in-a-Suitcase depends on using cold-chain-independent reagents. The electricity was supplied from a motor vehicle battery or solar panel battery. The H7N9 RT-RPA assays provided results within 10 min.

Development of portable and rapid assay for detection of emerging avian influenza A (H7N9) virus Read More

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