Publications

Every year more and more scientists are finding out that RPA really works. Over 200 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.
*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Rickettsia bacteria

    Development of a pan-rickettsial molecular diagnostic test based on recombinase polymerase amplification assay

    Author: Kissenkötter J, Hansen S, Böhlken-Fascher S, Ademowo OG, Oyinloye OE, Bakarey AS, Dobler G, Tappe D, Patel P, Czerny CP, Abd El Wahed A.
    All results were compared with real-time PCR assays directed against the same rickettsial genes. The RPA assays are easy to handle and produced quicker results in comparison to real-time PCR.
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  • Rapid and Sensitive Detection of Salmonella based on Microfluidic Enrichment with a Label-free Nanobiosensing Platform

    Rapid and Sensitive Detection of Salmonella based on Microfluidic Enrichment with a Label-free Nanobiosensing Platform

    Author: Nguyen DTT, Yoon J, Jin CE, Koo B, Han K, Shin Y, Lee TY .
    Highlights: Salmonella Typhimurium was detected in urine samples in real-time using a label-free method. •Sensitivity was improved by microfluidic enrichment. •The whole experiment was completed within 100 min.
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  • A microfluidic enrichment platform with a recombinase polymerase amplification sensor for pathogen diagnosis

    A microfluidic enrichment platform with a recombinase polymerase amplification sensor for pathogen diagnosis

    Author: Nguyen DTT, Lee EY, Koo B, Jin CE, Lee TY, Shin Y.
    Using this enrichment platform, the detection limit was found to be 20 times more sensitive in 10 ml urine with Salmonella and 10 times more sensitive in 10 ml urine with Brucella than that of real-time PCR without the enrichment process.
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  • Rapid and visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick

    Rapid and visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick.

    Author: Guimin Z, Hongmei W, Peili H, Chengqiang H, Hongbin H.
    The assay was specific, as it did not amplify genomes from five other Mycobacterium and five pathogenic enteric bacteria. Then, 612 clinical samples (320 fecal and 292 serum) were assessed by RPA-LFD, qPCR and ELISA assays respectively, also the established ...
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  • Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus

    Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus

    Author: Hou P, Zhao G, Wang H, He C, Huan Y, He H.
    The result showed that the coincidence rate of BEFV LFD-RPA and real-time qPCR was 96.09% (123/128), which was higher than conventional RT-PCR. The RPA combined with LFD assay probably provides a rapid and sensitive alternative for diagnosis of BEFV infections ...
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  • A DNA Barcode-Based RPA Assay (BAR-RPA) for Rapid Identification of the Dry Root of Ficus hirta (Wuzhimaotao)

    A DNA Barcode-Based RPA Assay (BAR-RPA) for Rapid Identification of the Dry Root of Ficus hirta (Wuzhimaotao)

    Author: Tian E, Liu Q, Ye H, Li F, Chao Z.
    This technique showed a high specificity and sensitivity to amplify the genomic DNA of F. hirta and allowed for rapid amplification (within 15 min) of the ITS region under a constant and mild temperature range of 37–42 °C without using ...
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  • Long-tailed macaque

    Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi

    Author: Lai MY, Ooi CH, Lau YL.
    With incubation at 37°C, the 18S rRNA gene of P. knowlesi was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip.
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  • Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene

    Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene

    Author: Martorell S, Palanca S, Maquieira A, Tortajada-Genaro LA.
    Herein, the detection of mutations in PIK3CA gene, such as p.E545K, and p.H1047L, is presented. The main element was an oligonucleotide (dideoxycytidine functionalized at 3′-end) which matched with wild-type sequence in the target locus.
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  • Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays

    Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays

    Author: Hou P, Wang H, Zhao G, He C, He H.
    The assay performance on acute-phase high fever clinical samples collected from cattle with no vaccine against IBRV, which were suspected to be infected with IBRV, was validated by detecting 24 fecal, 36 blood, 38 nasal swab and 8 tissue specimens, ...
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  • Rabies

    Real-time RPA assay for rapid detection and differentiation of wild-type pseudorabies and gE-deleted vaccine viruses

    Author: Wang J, Liu L, Wang J, Pang X, Yuan W.
    The analytical sensitivity of the assay was 102 copies for gB and gE genes. The dual real-time RPA demonstrated a 100% diagnostic agreement with the real-time PCR on 4 PRV strains and 37 clinical samples.
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