PublicationsPost Type
RPA (Recombinase Polymerase Amplification) combined with a novel bridging flocculation assay for qualitative evaluation of isothermally amplified plant and other pathogen DNA and RNA by naked eye.
We showed the ability of the MTB-ISAD assay to detect MTB in 42 clinical specimens, confirming that the MTB-ISAD assay is fast (90%, 38/42), and cost-effective because it is a label-free method and does not involve thermal cycling.
In this study, event-specific detection method for transgenic insect resistance rice Kefeng 6 and its derivates based on real-time fluorescent recombinase polymerase amplification (RPA) was established.
Event-specific Real-time RPA Detection of Transgenic Rice Kefeng 6 Read More
Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is responsible for significant economic losses in the shrimp aquaculture industry. Probit analysis of eight independent experimental replicates showed sufficient sensitivity to detect as few as 4 copies of the IHHNV genome within 7 min at 39 °C with 95 % reliability.
On-chip recombinase polymerase amplification (RPA) on defined spots of a DNA microarray was used to spatially separate the amplification reaction of DNA from two viruses (Human adenovirus 41, Phi X 174) and the bacterium Enterococcus faecalis, which are relevant for water hygiene. Within 48 min the microbes could be identified by the spot position on the microarray while the generated chemiluminescence
Orf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. A TwistAmp exo assay was capable of as low as 100 copies of ORFV DNA /reaction and was highly specific, with no cross-reaction with closely related viruses.
Development of a fluorescent probe-based RPA assay for rapid detection of Orf virus Read More
The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples.
Bridging flocculation assay for rapid detection of gene specific DNA methylation Read More
The assay is capable of detecting a positive differential pulse voltammetry (DPV) response from as low as 1 CFU of Mtb bacilli DNA input material, having shown its exquisite sensitivity over a conventional gel based readout. The translation of our assay onto a portable potentiostat was also demonstrated, with promising results. We believe that our assay has significant potential for translation into
Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine diagnostics and research.
Both isothermal amplification techniques show high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques is assessed in comparison to conventional PCR using biopsies from eumycetoma patients. RPA is reliable and easy to operate and has a potential to be implemented in mycetoma endemic areas.
