Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR.

*TwistDx takes no responsibility for the content of the publications or their author/s.

  • Bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings

    Author: E. J. H. Wee, H. Y. Lau, J. R. Botella and M. Trau

    RPA (Recombinase Polymerase Amplification) combined with a novel bridging flocculation assay for qualitative evaluation of isothermally amplified plant and other pathogen DNA and RNA by naked eye.

  • An RPA assay for analysis of Mycobacterium tuberculosis using a silicon bio-photonic sensor complex.

    Author: Shin Y, Perera AP, Tang WY, Fu DL, Liu Q, Sheng JK, Gu Z, Lee TY, Barkham T, Park MK

    We showed the ability of the MTB-ISAD assay to detect MTB in 42 clinical specimens, confirming that the MTB-ISAD assay is fast (90%, 38/42), and cost-effective because it is a label-free method and does not involve thermal cycling.

  • Event-specific Real-time RPA Detection of Transgenic Rice Kefeng 6

    Author: Chao Xu, Liang Li, Wujun Jin, Yusong Wan

    In this study, event-specific detection method for transgenic insect resistance rice Kefeng 6 and its derivates based on real-time fluorescent recombinase polymerase amplification (RPA) was established.

  • Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time RPA.

    Author: Xia X, Yu Y, Hu L, Weidmann M, Pan Y, Yan S, Wang Y.

    Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is responsible for significant economic losses in the shrimp aquaculture industry. Probit analysis of eight independent experimental replicates showed sufficient sensitivity to detect as few as 4 copies of the IHHNV genome within […]

  • On-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis

    Author: Kunze A, Dilcher M, Abd El Wahed A, Hufert F, Niessner R, Seidel M

    On-chip recombinase polymerase amplification (RPA) on defined spots of a DNA microarray was used to spatially separate the amplification reaction of DNA from two viruses (Human adenovirus 41, Phi X 174) and the bacterium Enterococcus faecalis, which are relevant for […]

  • Development of a fluorescent probe-based RPA assay for rapid detection of Orf virus

    Author: Yang Yang, Xiaodong Qin, Guangxiang Wang, Yuen Zhang, Youjun Shang and Zhidong Zhang

    Orf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. A TwistAmp exo assay was capable of as low as 100 copies […]

  • Bridging flocculation assay for rapid detection of gene specific DNA methylation

    Author: Eugene J. H. Wee, Thu Ha Ngo and Matt Trau

    The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) […]

  • Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Gold Nanoparticles

    Author: Benjamin Y. C. Ng, Wei Xiao, Nicholas P. West, Eugene J. H. Wee, Yuling Wang and Matt Trau

    The assay is capable of detecting a positive differential pulse voltammetry (DPV) response from as low as 1 CFU of Mtb bacilli DNA input material, having shown its exquisite sensitivity over a conventional gel based readout. The translation of our assay onto […]

  • Colorimetric detection of total genomic and loci-specific DNA methylation from limited DNA inputs

    Author: Eugene J. H. Wee, Thu Ha Ngo and Matt Trau

    Aberrant DNA methylation marks are potential disease biomarkers, and detecting both total genomic and gene-specific DNA methylation can aid in clinical decisions. While a plethora of methods exist in research, simpler, more convenient alternatives are needed to enhance both routine […]

  • Application of Isothermal Amplification Techniques for the Identification of Madurella mycetomatis

    Author: Sarah A. Ahmed, Wendy W. J. van de Sande, Marie Desnos-Ollivier, Ahmed H. Fahal, Najwa A. Mhmoud and G. S. de Hoog

    Both isothermal amplification techniques show high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques is assessed in comparison to conventional PCR using biopsies from eumycetoma patients. RPA […]

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