Publications
*TwistDx takes no responsibility for the content of the publications or their author/s.
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Rapid detection and differentiation of carp oedema virus and cyprinid herpes virus 3 in koi and common carp
Author: Soliman H, El-Matbouli M.The lower detection limits of the assays were similar to those of established diagnostic PCR tests for the viruses. A sample preparation method was optimized to eliminate the need for total DNA extraction from fish tissues. The estimated time to […]
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Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene
Author: Martorell S, Palanca S, Maquieira A, Tortajada-Genaro LA.Herein, the detection of mutations in PIK3CA gene, such as p.E545K, and p.H1047L, is presented. The main element was an oligonucleotide (dideoxycytidine functionalized at 3′-end) which matched with wild-type sequence in the target locus.
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Real-time RPA assay for rapid detection and differentiation of wild-type pseudorabies and gE-deleted vaccine viruses
Author: Wang J, Liu L, Wang J, Pang X, Yuan W.The analytical sensitivity of the assay was 102 copies for gB and gE genes. The dual real-time RPA demonstrated a 100% diagnostic agreement with the real-time PCR on 4 PRV strains and 37 clinical samples.
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Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
Author: Lai MY, Ooi CH, Lau YL.With incubation at 37°C, the 18S rRNA gene of P. knowlesi was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip.
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Development of a pan-rickettsial molecular diagnostic test based on recombinase polymerase amplification assay
Author: Kissenkötter J, Hansen S, Böhlken-Fascher S, Ademowo OG, Oyinloye OE, Bakarey AS, Dobler G, Tappe D, Patel P, Czerny CP, Abd El Wahed A.All results were compared with real-time PCR assays directed against the same rickettsial genes. The RPA assays are easy to handle and produced quicker results in comparison to real-time PCR.
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A microfluidic enrichment platform with a recombinase polymerase amplification sensor for pathogen diagnosis
Author: Nguyen DTT, Lee EY, Koo B, Jin CE, Lee TY, Shin Y.Using this enrichment platform, the detection limit was found to be 20 times more sensitive in 10 ml urine with Salmonella and 10 times more sensitive in 10 ml urine with Brucella than that of real-time PCR without the enrichment process.
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Rapid and visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick.
Author: Guimin Z, Hongmei W, Peili H, Chengqiang H, Hongbin H.The assay was specific, as it did not amplify genomes from five other Mycobacterium and five pathogenic enteric bacteria. Then, 612 clinical samples (320 fecal and 292 serum) were assessed by RPA-LFD, qPCR and ELISA assays respectively, also the established […]
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Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus
Author: Hou P, Zhao G, Wang H, He C, Huan Y, He H.The result showed that the coincidence rate of BEFV LFD-RPA and real-time qPCR was 96.09% (123/128), which was higher than conventional RT-PCR. The RPA combined with LFD assay probably provides a rapid and sensitive alternative for diagnosis of BEFV infections […]
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Automated real-time detection of drug-resistant Mycobacterium tuberculosis on a lab-on-a-disc by Recombinase Polymerase Amplification
Author: Law ILG, Loo JFC, Kwok HC, Yeung HY, Leung CCH, Hui M, Wu SY, Chan HS, Kwan YW, Ho HP, Kong SK.In our platform, real-time RPA (RT-RPA) was integrated on a lab-on-a-disc (LOAD) with on-board power to maintain temperature for DNA amplification. Sputa collected from healthy volunteers were spiked with respective target M. tb samples for testing. A limit of detection […]
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Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays
Author: Hou P, Wang H, Zhao G, He C, He H.The assay performance on acute-phase high fever clinical samples collected from cattle with no vaccine against IBRV, which were suspected to be infected with IBRV, was validated by detecting 24 fecal, 36 blood, 38 nasal swab and 8 tissue specimens, […]